Type IV B pili are required for invasion but not for adhesion of Salmonella enterica serovar Typhi into BHK epithelial cells in a cystic fibrosis transmembrane conductance regulator-independent manner

Microbial Pathogenesis

Volumn 51, Issue 5, 2011, Pages 373-377

  Bravo, Denisse ^a   Blondel, Carlos J ^a   Hoare, Anilei ^a   Leyton, Lisette ^b   Valvano, Miguel A ^c   Contreras, Inés ^a  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSITY OF CHILE   (Chile)

^c UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

Cystic fibrosis transmembrane conductance regulator; Epithelial cell invasion; Lipopolysaccharide; Salmonella; Type IV pili

Indexed keywords

MUTANT PROTEIN; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM PILUS; CELL INVASION; CELL SURFACE; CONTROLLED STUDY; EPITHELIUM CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; SALMONELLA TYPHI; SALMONELLA TYPHIMURIUM; SEROTYPE; STRAIN DIFFERENCE; WILD TYPE;

ANIMALS; BACTERIAL ADHESION; CELL LINE; CRICETINAE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; FIMBRIAE, BACTERIAL; HUMANS; SALMONELLA INFECTIONS; SALMONELLA TYPHI; SALMONELLA TYPHIMURIUM; VIRULENCE;

BACTERIA (MICROORGANISMS); SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHI; SALMONELLA TYPHI; SALMONELLA TYPHIMURIUM;

EID: 80052492301     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2011.07.005     Document Type: Article

References (29)

1. Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Shapiro C., et al. Food-related illness and death in the United States. Emerg Infect Dis 1999, 5:607-625.
2. Wain J., House D., Parkhill J., Parry C., Dougan G. Unlocking the genome of the human typhoid bacillus. Lancet Infect Dis 2002, 2:163-170.
3. Levine M.M., Sztein B. Shigella, Salmonella Typhi, and Escherichia coli. MWCaRSF, book 2000, 171-194. Williams & Wilkins, Philadelphia, Pa.29 Lippincott.
4. Finlay B.B., Heffron F., Falkow S. Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion. Science 1989, 243:940-943.
5. Gewirtz A.T., Navas T.A., Lyons S., Godowski P.J., Madara J.L. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol 2001, 167:1882-1885.
6. Vishnyakova T.G., Kurlander R., Bocharov A.V., Baranova I.N., Chen Z., Abu-Asab M.S., et al. CLA-1 and its splicing variant CLA-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells. Proc Natl Acad Sci U S A 2006, 103:16888-16893.
7. Pier G.B., Grout M., Zaidi T., Meluleni G., Mueschenborn S.S., Banting G., et al. Salmonella Typhi uses CFTR to enter intestinal epithelial cells. Nature 1998, 393:79-82.
8. Lyczak J.B., Zaidi T.S., Grout M., Bittner M., Contreras I., Pier G.B. Epithelial cell contact-induced alterations in Salmonella enterica serovar Typhi lipopolysaccharide are critical for bacterial internalization. Cell Microbiol 2001, 3:763-772.
9. Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., et al. Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18. Nature 2001, 413:848-852.
10. McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., et al. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 2001, 413:852-856.
11. Bravo D., Hoare A., Silipo A., Valenzuela C., Salinas C., Alvarez S.A., et al. Different sugar residues of the lipopolysaccharide outer core are required for early interactions of Salmonella enterica serovars Typhi and Typhimurium with epithelial cells. Microb Pathog 2011, 50:70-80.
12. Tsui I.S., Yip C.M., Hackett J., Morris C. The type IVB pili of Salmonella enterica serovar Typhi bind to the cystic fibrosis transmembrane conductance regulator. Infect Immun 2003, 71:6049-6050.
13. Cheng S.H., Gregory R.J., Marshall J., Paul S., Souza D.W., White G.A., et al. Defective intracellular transport and processing of CFTR is the molecular basis of most cystic fibrosis. Cell 1990, 63:827-834.
14. Kalin N., Claass A., Sommer M., Puchelle E., Tummler B. DeltaF508 CFTR protein expression in tissues from patients with cystic fibrosis. J Clin Invest 1999, 103:1379-1389.
15. Kartner N., Augustinas O., Jensen T.J., Naismith A.L., Riordan J.R. Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland. Nat Genet 1992, 1:321-327.
16. Lukacs G.L., Mohamed A., Kartner N., Chang X.B., Riordan J.R., Grinstein S. Conformational maturation of CFTR but not its mutant counterpart (delta F508) occurs in the endoplasmic reticulum and requires ATP. EMBO J 1994, 13:6076-6086.
17. Mendes F., Wakefield J., Bachhuber T., Barroso M., Bebok Z., Penque D., et al. Establishment and characterization of a novel polarized MDCK epithelial cellular model for CFTR studies. Cell Physiol Biochem 2005, 16:281-290.
18. Denning G.M., Ostedgaard L.S., Welsh M.J. Abnormal localization of cystic fibrosis transmembrane conductance regulator in primary cultures of cystic fibrosis airway epithelia. J Cell Biol 1992, 118:551-559.
19. Sharma M., Benharouga M., Hu W., Lukacs G.L. Conformational and temperature- sensitive stability defects of the delta F508 cystic fibrosis transmembrane conductance regulator in post-endoplasmic reticulum compartments. J Biol Chem 2001, 276:8942-8950.
20. Du K., Sharma M., Lukacs G.L. The DeltaF508 cystic fibrosis mutation impairs domain-domain interactions and arrests post-translational folding of CFTR. Nat Struct Mol Biol 2005, 12:17-25.
21. Zhang F., Kartner N., Lukacs G.L. Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR. Nat Struct Biol 1998, 5:180-183.
22. Ward C.L., Kopito R.R. Intracellular turnover of cystic fibrosis transmembrane conductance regulator. Inefficient processing and rapid degradation of wild-type and mutant proteins. J Biol Chem 1994, 269:25710-25718.
23. Ward C.L., Omura S., Kopito R.R. Degradation of CFTR by the ubiquitin-proteasome pathway. Cell 1995, 83:121-127.
24. Jensen T.J., Loo M.A., Pind S., Williams D.B., Goldberg A.L., Riordan J.R. Multiple proteolytic systems, including the proteasome, contribute to CFTR processing. Cell 1995, 83:129-135.
25. Lukacs G.L., Chang X.B., Bear C., Kartner N., Mohamed A., Riordan J.R., et al. The delta F508 mutation decreases the stability of cystic fibrosis transmembrane conductance regulator in the plasma membrane. Determination of functional half- lives on transfected cells. J Biol Chem 1993, 268:21592-21598.
26. Zhang X.L., Tsui I.S., Yip C.M., Fung A.W., Wong D.K., Dai X., et al. Salmonella enterica serovar Typhi uses type IVB pili to enter human intestinal epithelial cells. Infect Immun 2000, 68:3067-3073.
27. Bueno S.M., Santiviago C.A., Murillo A.A., Fuentes J.A., Trombert A.N., Rodas P.I., et al. Precise excision of the large pathogenicity island, SPI7, in Salmonella enterica serovar Typhi. J Bacteriol 2004, 186:3202-3213.
28. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 2000, 97:6640-6645.
29. Deng W., Liou S.R., Plunkett G., Mayhew G.F., Rose D.J., Burland V., et al. Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18. J Bacteriol 2003, 185:2330-2337.