메뉴 건너뛰기




Volumn 58, Issue 4, 2011, Pages 380-388

Antibodies anti-Shiga toxin 2 B subunit from chicken egg yolk: Isolation, purification and neutralization efficacy

Author keywords

Antitoxin; Chicken; IgY technology; Neutralization; Shiga toxin; Stx2B

Indexed keywords

IMMUNOGLOBULIN G; IMMUNOGLOBULIN Y; POLYCLONAL ANTIBODY; TOXIN ANTIBODY; VEROTOXIN 2;

EID: 80052358549     PISSN: 00410101     EISSN: 18793150     Source Type: Journal    
DOI: 10.1016/j.toxicon.2011.07.009     Document Type: Article
Times cited : (29)

References (52)
  • 2
    • 84987350951 scopus 로고
    • Immunoglobulins from egg yolk: Isolation and purification
    • Akita E.M., Nakai S. Immunoglobulins from egg yolk: Isolation and purification. Food Sci. 1992, 57:629-634.
    • (1992) Food Sci. , vol.57 , pp. 629-634
    • Akita, E.M.1    Nakai, S.2
  • 3
    • 13144292220 scopus 로고
    • Studies on organisms concerned as causative factors in botulism
    • Bengtson I.A. Studies on organisms concerned as causative factors in botulism. Hyg. Lab. Bull. 1924, 136:1-97.
    • (1924) Hyg. Lab. Bull. , vol.136 , pp. 1-97
    • Bengtson, I.A.1
  • 4
    • 0034145668 scopus 로고    scopus 로고
    • A comparison of three methods for extracting IgY from the egg yolk of hens immunized with Sendai virus
    • Bizhanov G., Vyshniauskis G. A comparison of three methods for extracting IgY from the egg yolk of hens immunized with Sendai virus. Vet. Res. Commun. 2000, 24:103-113.
    • (2000) Vet. Res. Commun. , vol.24 , pp. 103-113
    • Bizhanov, G.1    Vyshniauskis, G.2
  • 5
    • 70350041489 scopus 로고    scopus 로고
    • Recommendations for diagnosis of Shiga Toxin-Producing Escherichia coli infections by clinical Laboratories
    • CDC
    • Recommendations for diagnosis of Shiga Toxin-Producing Escherichia coli infections by clinical Laboratories. MMWR 2009, 58:1-14. CDC.
    • (2009) MMWR , vol.58 , pp. 1-14
  • 6
    • 0033007234 scopus 로고    scopus 로고
    • Antibody based protection of gnotobiotic piglets infected with Escherichia coli O157:H7 against systemic complications associated with Shiga toxin 2
    • Donohue-Rolfe A., Kondova I., Mukherjee J., Chios K., Hutto D., Tzipori S. Antibody based protection of gnotobiotic piglets infected with Escherichia coli O157:H7 against systemic complications associated with Shiga toxin 2. Infect. Immun. 1999, 67:3645-3648.
    • (1999) Infect. Immun. , vol.67 , pp. 3645-3648
    • Donohue-Rolfe, A.1    Kondova, I.2    Mukherjee, J.3    Chios, K.4    Hutto, D.5    Tzipori, S.6
  • 7
    • 0025279437 scopus 로고
    • Efficient production of chicken egg yolk antibodies against a conserved mammalian protein
    • Gassmann M., Thömmes P., Weiser T., Hübscher U. Efficient production of chicken egg yolk antibodies against a conserved mammalian protein. FASEB J. 1990, 4:2528-2532.
    • (1990) FASEB J. , vol.4 , pp. 2528-2532
    • Gassmann, M.1    Thömmes, P.2    Weiser, T.3    Hübscher, U.4
  • 8
    • 0018826096 scopus 로고
    • Quantitative microtiter cytotoxicity assay for Shigella toxin
    • Gentry M.K., Dalrymple J.M. Quantitative microtiter cytotoxicity assay for Shigella toxin. J. Clin. Microbiol. 1980, 12:361-366.
    • (1980) J. Clin. Microbiol. , vol.12 , pp. 361-366
    • Gentry, M.K.1    Dalrymple, J.M.2
  • 10
    • 0000047520 scopus 로고
    • Escherichia coli O157:H7 and other enterohaemorrhagic Escherichia coli
    • Raven Press, New York, M.J. Blaser, P.D. Smith, J.I. Ravdin, H.B. Greenberg, R.L. Guerrant (Eds.)
    • Griffin P.M. Escherichia coli O157:H7 and other enterohaemorrhagic Escherichia coli. Infections of the Gastrointestinal Tract N 1995, 739-761. Raven Press, New York. M.J. Blaser, P.D. Smith, J.I. Ravdin, H.B. Greenberg, R.L. Guerrant (Eds.).
    • (1995) Infections of the Gastrointestinal Tract N , pp. 739-761
    • Griffin, P.M.1
  • 12
    • 78149336370 scopus 로고    scopus 로고
    • Shiga toxin B subunits induce VWF secretion by human endothelial cells and thrombotic microangiopathy in ADAMTS13-deficient mice
    • Huang J., Motto D., Bundle D., Sadler E. Shiga toxin B subunits induce VWF secretion by human endothelial cells and thrombotic microangiopathy in ADAMTS13-deficient mice. Blood 2010, 116:3653-3659.
    • (2010) Blood , vol.116 , pp. 3653-3659
    • Huang, J.1    Motto, D.2    Bundle, D.3    Sadler, E.4
  • 13
    • 0842326291 scopus 로고    scopus 로고
    • Production of secretory immunoglobulin A against Shiga toxin-binding subunits in mice by mucosal immunization
    • Imai Y., Nagai R., Ono Y., Ishikawa T., Nakagami H., Tanikawa T., Kurohane K. Production of secretory immunoglobulin A against Shiga toxin-binding subunits in mice by mucosal immunization. Infect. Immun. 2004, 72:889-895.
    • (2004) Infect. Immun. , vol.72 , pp. 889-895
    • Imai, Y.1    Nagai, R.2    Ono, Y.3    Ishikawa, T.4    Nakagami, H.5    Tanikawa, T.6    Kurohane, K.7
  • 14
    • 0030959865 scopus 로고    scopus 로고
    • Chicken egg yolk antibodies against F18 ab fimbriae of Escherichia coli inhibit shedding of F18 positive E. coli by experimentally infected pigs
    • Imberechts H., Deprez P., Van Driessche E., Pohl P. Chicken egg yolk antibodies against F18 ab fimbriae of Escherichia coli inhibit shedding of F18 positive E. coli by experimentally infected pigs. Vet. Microbiol. 1997, 54:329-341.
    • (1997) Vet. Microbiol. , vol.54 , pp. 329-341
    • Imberechts, H.1    Deprez, P.2    Van Driessche, E.3    Pohl, P.4
  • 15
    • 77952272576 scopus 로고    scopus 로고
    • In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2
    • Jeong K.I., Chapman-Bonofiglio S., Singh P., Lee J., Tzipori S., Sheoran A.S. In vitro and in vivo protective efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga toxin 2. BMC Immunol. 2010, 11:16.
    • (2010) BMC Immunol. , vol.11 , pp. 16
    • Jeong, K.I.1    Chapman-Bonofiglio, S.2    Singh, P.3    Lee, J.4    Tzipori, S.5    Sheoran, A.S.6
  • 16
    • 0036087654 scopus 로고    scopus 로고
    • The epithelial cell cytoskeleton and intracellular trafficking. I. Shiga toxin B-subunit system: retrograde transport, intracellular vectorization, and more
    • Johannes L. The epithelial cell cytoskeleton and intracellular trafficking. I. Shiga toxin B-subunit system: retrograde transport, intracellular vectorization, and more. Am. J. Physiol. Gastrointest. Liver Physiol. 2002, 283:G1-G7.
    • (2002) Am. J. Physiol. Gastrointest. Liver Physiol. , vol.283
    • Johannes, L.1
  • 18
    • 0021946215 scopus 로고
    • The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli
    • Karmali M.A., Petric M., Lim C., Fleming P.C., Arbus G.S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli. J. Infect. Dis. 1985, 151:775-782.
    • (1985) J. Infect. Dis. , vol.151 , pp. 775-782
    • Karmali, M.A.1    Petric, M.2    Lim, C.3    Fleming, P.C.4    Arbus, G.S.5    Lior, H.6
  • 19
    • 77954448751 scopus 로고    scopus 로고
    • Performance of an enzyme-linked immunosorbent assay for detection of Clonorchis sinensis infestation in high- and low-risk groups
    • Kim Y.J., Lee S.M., Choi G.E., Hwang S.H., Kim H.H., Lee E.Y., Chang C.L. Performance of an enzyme-linked immunosorbent assay for detection of Clonorchis sinensis infestation in high- and low-risk groups. J. Clin. Microbiol. 2010, 48:2365-2367.
    • (2010) J. Clin. Microbiol. , vol.48 , pp. 2365-2367
    • Kim, Y.J.1    Lee, S.M.2    Choi, G.E.3    Hwang, S.H.4    Kim, H.H.5    Lee, E.Y.6    Chang, C.L.7
  • 20
    • 0023687040 scopus 로고
    • The primary structure of the operons coding for Shigella dysenteriae toxin and temperature phage.H30 shiga-like toxin
    • Kozlov Y.V., Kabishev A.A., Lukyanov E.V., Bayev A.A. The primary structure of the operons coding for Shigella dysenteriae toxin and temperature phage.H30 shiga-like toxin. Gene 1988, 67:213-221.
    • (1988) Gene , vol.67 , pp. 213-221
    • Kozlov, Y.V.1    Kabishev, A.A.2    Lukyanov, E.V.3    Bayev, A.A.4
  • 21
    • 79251610299 scopus 로고    scopus 로고
    • Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: type, number of variants, and relationship to cytotoxicity
    • Krüger A., Lucchesi P.M., Parma A.E. Verotoxins in bovine and meat verotoxin-producing Escherichia coli isolates: type, number of variants, and relationship to cytotoxicity. Appl. Environ. Microbiol. 2011, 77:73-79.
    • (2011) Appl. Environ. Microbiol. , vol.77 , pp. 73-79
    • Krüger, A.1    Lucchesi, P.M.2    Parma, A.E.3
  • 22
    • 77954216766 scopus 로고    scopus 로고
    • Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7
    • Krystle L.M., Melton-Celsa A.R., Robinson C.M., O'Brien A.D. Neutralizing antibodies to Shiga toxin type 2 (Stx2) reduce colonization of mice by Stx2-expressing Escherichia coli O157:H7. Vaccine 2010, 28:4777-4785.
    • (2010) Vaccine , vol.28 , pp. 4777-4785
    • Krystle, L.M.1    Melton-Celsa, A.R.2    Robinson, C.M.3    O'Brien, A.D.4
  • 23
    • 0034279899 scopus 로고    scopus 로고
    • Immunoprophylactic effect of chicken egg yolk immunoglobulin (IgY) against porcine epidemic diarrhea virus (PEDV) in piglets
    • Kweon C.H., Kwon B.J., Woo S.R., Kim J.M., Woo G.H., Son D.H., Hur W., Lee Y.S. Immunoprophylactic effect of chicken egg yolk immunoglobulin (IgY) against porcine epidemic diarrhea virus (PEDV) in piglets. J. Vet. Med. Sci. 2000, 62:961-964.
    • (2000) J. Vet. Med. Sci. , vol.62 , pp. 961-964
    • Kweon, C.H.1    Kwon, B.J.2    Woo, S.R.3    Kim, J.M.4    Woo, G.H.5    Son, D.H.6    Hur, W.7    Lee, Y.S.8
  • 24
    • 0036117817 scopus 로고    scopus 로고
    • Protection against bacterial staphylococcal enterotoxin B by passive vaccination
    • LeClaire R.D., Hunt R.E., Bavari S. Protection against bacterial staphylococcal enterotoxin B by passive vaccination. Infect. Immun. 2002, 70:2278-2281.
    • (2002) Infect. Immun. , vol.70 , pp. 2278-2281
    • LeClaire, R.D.1    Hunt, R.E.2    Bavari, S.3
  • 25
    • 0036562243 scopus 로고    scopus 로고
    • Vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium
    • Lee E.N., Sunwoo H., Menninen K., Sim J.S. Vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poult. Sci. 2002, 81:632-641.
    • (2002) Poult. Sci. , vol.81 , pp. 632-641
    • Lee, E.N.1    Sunwoo, H.2    Menninen, K.3    Sim, J.S.4
  • 28
    • 25444471025 scopus 로고    scopus 로고
    • Recombinant shiga toxin B-Subunit-Keyhole Limpet Hemocyanin Conjugate Vaccine protects mice from Shigatoxemia
    • Marcato P., Griener T., Mulvey G., Armstrong G. Recombinant shiga toxin B-Subunit-Keyhole Limpet Hemocyanin Conjugate Vaccine protects mice from Shigatoxemia. Infect. Immunity 2005, 73:6523-6529.
    • (2005) Infect. Immunity , vol.73 , pp. 6523-6529
    • Marcato, P.1    Griener, T.2    Mulvey, G.3    Armstrong, G.4
  • 29
    • 0029035219 scopus 로고
    • An alternative approach to the optimal design of an LD50 bioassay
    • Markus R.A., Frank J., Groshen S., Azen S.P. An alternative approach to the optimal design of an LD50 bioassay. Stat. Med. 1995, 14:841-852.
    • (1995) Stat. Med. , vol.14 , pp. 841-852
    • Markus, R.A.1    Frank, J.2    Groshen, S.3    Azen, S.P.4
  • 30
    • 0032940289 scopus 로고    scopus 로고
    • Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets
    • Marquardt R., Jin L.Z., Kim J.W., Fang L., Frohlich A., Baidoo S. Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets. FEMS Immunol. Med. Microbiol. 1999, 23:283-288.
    • (1999) FEMS Immunol. Med. Microbiol. , vol.23 , pp. 283-288
    • Marquardt, R.1    Jin, L.Z.2    Kim, J.W.3    Fang, L.4    Frohlich, A.5    Baidoo, S.6
  • 31
    • 0036754686 scopus 로고    scopus 로고
    • Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review
    • Mine Y., Kovacs-Nolan J. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review. J. Med. Food 2002, 5:159-169.
    • (2002) J. Med. Food , vol.5 , pp. 159-169
    • Mine, Y.1    Kovacs-Nolan, J.2
  • 32
    • 16244382346 scopus 로고    scopus 로고
    • Production of rabies neutralizing antibody in hen's eggs using a part of the G protein expressed in Escherichia coli
    • Motoi Y., Sato K., Hatta H., Morimoto K., Inoue S., Yamada A. Production of rabies neutralizing antibody in hen's eggs using a part of the G protein expressed in Escherichia coli. Vaccine 2005, 23:3026-3032.
    • (2005) Vaccine , vol.23 , pp. 3026-3032
    • Motoi, Y.1    Sato, K.2    Hatta, H.3    Morimoto, K.4    Inoue, S.5    Yamada, A.6
  • 34
    • 40749130330 scopus 로고    scopus 로고
    • Prevention and treatment of enterohemorrhagic Escherichia coli infections in humans
    • Orth D., Grif K., Zimmerhackl L.B., Würzner R. Prevention and treatment of enterohemorrhagic Escherichia coli infections in humans. Expert Rev. Anti-Infect. Ther. 2008, 6:101-108.
    • (2008) Expert Rev. Anti-Infect. Ther. , vol.6 , pp. 101-108
    • Orth, D.1    Grif, K.2    Zimmerhackl, L.B.3    Würzner, R.4
  • 35
    • 0034529570 scopus 로고    scopus 로고
    • Virulence genotypes and serotypes of verotoxigenic Escherichia coli isolated from cattle and foods in Argentina. Importance in public health
    • Parma A.E., Sanz M.E., Blanco J.E., Blanco J., Viñas M.R., Blanco M., Padola N.L., Etcheverría A.I. Virulence genotypes and serotypes of verotoxigenic Escherichia coli isolated from cattle and foods in Argentina. Importance in public health. Europ. J. Epidemiol. E. 2000, 16:757-762.
    • (2000) Europ. J. Epidemiol. E. , vol.16 , pp. 757-762
    • Parma, A.E.1    Sanz, M.E.2    Blanco, J.E.3    Blanco, J.4    Viñas, M.R.5    Blanco, M.6    Padola, N.L.7    Etcheverría, A.I.8
  • 36
    • 63249105438 scopus 로고    scopus 로고
    • Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period
    • Pauly D., Dorner M., Zhang X., Hlinak A., Dorner B., Schade R. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period. Poult. Sci. 2009, 88:281-290.
    • (2009) Poult. Sci. , vol.88 , pp. 281-290
    • Pauly, D.1    Dorner, M.2    Zhang, X.3    Hlinak, A.4    Dorner, B.5    Schade, R.6
  • 37
    • 33745158157 scopus 로고
    • A simple method of estimating fifty per cent endpoints
    • Reed L., Muench H. A simple method of estimating fifty per cent endpoints. Am. J. Hyg. 1938, 27:493-497.
    • (1938) Am. J. Hyg. , vol.27 , pp. 493-497
    • Reed, L.1    Muench, H.2
  • 38
    • 80052343857 scopus 로고    scopus 로고
    • Chapter 10: diarrheagenic Escherichia coli in Argentina
    • Bentham Science Publishers Ltd, Oak Park (IL), Estados Unidos, A.G. Torres (Ed.)
    • Rivas M., Padola N.L., Lucchesi P.M.A., Massana M. Chapter 10: diarrheagenic Escherichia coli in Argentina. Pathogenic Escherichia coli in Latin America 2010, pp. 142-161, Bentham Science Publishers Ltd, Oak Park (IL), Estados Unidos. A.G. Torres (Ed.).
    • (2010) Pathogenic Escherichia coli in Latin America , pp. 142-161
    • Rivas, M.1    Padola, N.L.2    Lucchesi, P.M.A.3    Massana, M.4
  • 39
    • 0002531354 scopus 로고    scopus 로고
    • Specificity of chicken (IgY) versus rabbit (IgG) antibodies raised against cholecystokinin octapeptide (CCK-8)
    • Schade R., Henklein P., Hlinak A., de Vente J., Steinbusch H. Specificity of chicken (IgY) versus rabbit (IgG) antibodies raised against cholecystokinin octapeptide (CCK-8). ALTEX 1996, 13(Suppl.):80-85.
    • (1996) ALTEX , vol.13 , Issue.SUPPL. , pp. 80-85
    • Schade, R.1    Henklein, P.2    Hlinak, A.3    de Vente, J.4    Steinbusch, H.5
  • 40
    • 18844458814 scopus 로고    scopus 로고
    • Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine
    • Schade R., Calzado E.G., Sarmiento R., Chacana P.A., Porankiewicz-Asplund J., Terzolo H.R. Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine. Altern. Lab. Anim. 2005, 33:129-154.
    • (2005) Altern. Lab. Anim. , vol.33 , pp. 129-154
    • Schade, R.1    Calzado, E.G.2    Sarmiento, R.3    Chacana, P.A.4    Porankiewicz-Asplund, J.5    Terzolo, H.R.6
  • 41
    • 84900945039 scopus 로고    scopus 로고
    • Egg yolk compounds - Livetin fractions
    • Springer, Germany, R. Huopalahti, R. Lopez- Fandiño, M. Antón, R. Schade (Eds.)
    • Schade R., Chacana P.A. Egg yolk compounds - Livetin fractions. En: Bioactive Egg Compounds 2007, 25-32. Springer, Germany. R. Huopalahti, R. Lopez- Fandiño, M. Antón, R. Schade (Eds.).
    • (2007) En: Bioactive Egg Compounds , pp. 25-32
    • Schade, R.1    Chacana, P.A.2
  • 42
    • 0036731908 scopus 로고    scopus 로고
    • Use of egg yolk-derived immunoglobulin as an alternative to Antibiotic treatment for control of Helicobacter pylori infection
    • Shin J.H., Yang M., Nam S.W., Kim J.T., Myung N.H., Bang W.G., Roe I.H. Use of egg yolk-derived immunoglobulin as an alternative to Antibiotic treatment for control of Helicobacter pylori infection. Clin. Diagn. Lab. Immunol. 2002, 9:1061-1066.
    • (2002) Clin. Diagn. Lab. Immunol. , vol.9 , pp. 1061-1066
    • Shin, J.H.1    Yang, M.2    Nam, S.W.3    Kim, J.T.4    Myung, N.H.5    Bang, W.G.6    Roe, I.H.7
  • 43
    • 0035064161 scopus 로고    scopus 로고
    • Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries
    • Smith D.J., King W.F., Godiska R. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect. Immun. 2001, 69:3135-3142.
    • (2001) Infect. Immun. , vol.69 , pp. 3135-3142
    • Smith, D.J.1    King, W.F.2    Godiska, R.3
  • 45
    • 33747084410 scopus 로고    scopus 로고
    • Detection of Escherichia coli 0157:H7 using chicken immunoglobulin Y
    • Sunwoo H., Wang W., Sim J. Detection of Escherichia coli 0157:H7 using chicken immunoglobulin Y. Immunol. Lett. 2006, 106:191-193.
    • (2006) Immunol. Lett. , vol.106 , pp. 191-193
    • Sunwoo, H.1    Wang, W.2    Sim, J.3
  • 46
    • 0027178560 scopus 로고
    • Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice
    • Tesh V.L., Burris J.A., Owens J.W. Comparison of the relative toxicities of Shiga-like toxins type I and type II for mice. Infect. Immun. 1993, 61:3392-3402.
    • (1993) Infect. Immun. , vol.61 , pp. 3392-3402
    • Tesh, V.L.1    Burris, J.A.2    Owens, J.W.3
  • 50
    • 0035884459 scopus 로고    scopus 로고
    • Efficacy of postinfection treatment with anti-Shiga toxin (Stx) 2 humanized monoclonal antibody TMA-15 in mice lethally challenged with Stx-producing Escherichia coli
    • Yamagami S., Motoki M., Kimura T., Izumi H., Takeda T., Katsuura Y. Efficacy of postinfection treatment with anti-Shiga toxin (Stx) 2 humanized monoclonal antibody TMA-15 in mice lethally challenged with Stx-producing Escherichia coli. J. Infect. Dis. 2001, 184:738-742.
    • (2001) J. Infect. Dis. , vol.184 , pp. 738-742
    • Yamagami, S.1    Motoki, M.2    Kimura, T.3    Izumi, H.4    Takeda, T.5    Katsuura, Y.6
  • 51
    • 36649031219 scopus 로고    scopus 로고
    • Chicken-derived IgY recognizes developing and mature stages of Loma salmonae (Microsporidia) in Pacific salmon, Oncorhynchus spp
    • Young C.A., Silversides F.G., Jones R.M. Chicken-derived IgY recognizes developing and mature stages of Loma salmonae (Microsporidia) in Pacific salmon, Oncorhynchus spp. Aquaculture 2007, 273:398-404.
    • (2007) Aquaculture , vol.273 , pp. 398-404
    • Young, C.A.1    Silversides, F.G.2    Jones, R.M.3
  • 52
    • 0037446308 scopus 로고    scopus 로고
    • The use of gene specific-IgY antibodies for drug target discovery
    • Zhang W. The use of gene specific-IgY antibodies for drug target discovery. Drug Discov. 2003, 8:364-371.
    • (2003) Drug Discov. , vol.8 , pp. 364-371
    • Zhang, W.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.