Development of a homogeneous fluorescence anisotropy assay to monitor and measure FtsZ assembly in solution

Analytical Biochemistry

Volumn 418, Issue 1, 2011, Pages 89-96

  Reija, Belén ^a   Monterroso, Begoña ^b   Jiménez, Mercedes ^b   Vicente, Miguel ^c   Rivas, Germán ^b   Zorrilla, Silvia ^a  

^a INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^c CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Bacterial cell division; Cooperative protein polymerization; Critical concentration; Fluorescence correlation spectroscopy; Time resolved fluorescence spectroscopy

Indexed keywords

ANISOTROPY; CELL PROLIFERATION; FLUORESCENCE SPECTROSCOPY; POLYMERIZATION; PROTEINS; SPECTROSCOPIC ANALYSIS;

BACTERIAL CELL DIVISION; CRITICAL CONCENTRATION; FLUORESCENCE ANISOTROPY; FLUORESCENCE ANISOTROPY METHODS; FLUORESCENCE CORRELATION SPECTROSCOPY; STEADY STATE FLUORESCENCES; TIME-RESOLVED FLUORESCENCE; TIME-RESOLVED FLUORESCENCE SPECTROSCOPY;

FLUORESCENCE;

FICOLL; FTSZ PROTEIN; MAGNESIUM ION; TRACER;

ANISOTROPY; ARTICLE; CONTROLLED STUDY; EMISSION ANISOTROPY; FLUORESCENCE ANALYSIS; FLUORESCENCE CORRELATION SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN POLYMERIZATION; PROTEIN PURIFICATION; RELIABILITY; SENSITIVITY AND SPECIFICITY; STEADY STATE;

ALEXA; BACTERIA (MICROORGANISMS); PROKARYOTA;

EID: 80052265964     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.07.001     Document Type: Article

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