Polymerase chain reaction-based detection of Pneumocystis jirovecii in bronchoalveolar lavage fluid for the diagnosis of pneumocystis pneumonia

American Journal of the Medical Sciences

Volumn 342, Issue 3, 2011, Pages 182-185

  Oren, Ilana ^a,c   Hardak, Emilia ^b   Finkelstein, Renato ^a,c   Yigla, Mordechai ^b,c   Sprecher, Hannah ^b  

^a Infectious Diseases Unit   (Israel)

^b Rambam Health Care Center   (Israel)

^c TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

Immunocompromised host; Pneumocystis jirovecii; Pneumocystis pneumonia; Pneumonia

Indexed keywords

IMMUNOSUPPRESSIVE AGENT;

ACUTE GRANULOCYTIC LEUKEMIA; ACUTE LYMPHOBLASTIC LEUKEMIA; ADOLESCENT; ADULT; AGED; ALLOGENIC BONE MARROW TRANSPLANTATION; ARTICLE; AUTOLOGOUS BONE MARROW TRANSPLANTATION; BONE MARROW TRANSPLANTATION; CHILD; CHRONIC LYMPHATIC LEUKEMIA; CHRONIC MYELOID LEUKEMIA; COHORT ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; DIAGNOSTIC TEST ACCURACY STUDY; DIFFERENTIAL DIAGNOSIS; FEMALE; FIBEROPTIC BRONCHOSCOPY; FUNGAL DETECTION; GENE TARGETING; HISTOPATHOLOGY; HUMAN; IMMUNOCOMPROMISED PATIENT; IMMUNOSUPPRESSIVE TREATMENT; INFANT; LUNG INFILTRATE; LUNG LAVAGE; LYMPHOMA; MAJOR CLINICAL STUDY; MALE; MICROORGANISM; MULTIPLE MYELOMA; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRESCHOOL CHILD; RISK FACTOR; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; STAINING;

EID: 80052259395     PISSN: 00029629     EISSN: 15382990     Source Type: Journal    
DOI: 10.1097/MAJ.0b013e318210ff42     Document Type: Article

References (20)

1. Thomas CF Jr, Limper AH. Pneumocystis pneumonia. N Engl J Med 2004;350:2487-98.
2. Bedrossian CW, Mason MR, Gupta PK. Rapid cytologic diagnosis of Pneumocystis: A comparison of effective techniques. Semin Diagn Pathol 1989;6:245-61. (Pubitemid 19207008)
3. Wakefield AE, Pixley FJ, Banerji S, et al. Detection of Pneumocystis carinii with DNA amplification. Lancet 1990;336:451-3. (Pubitemid 20264212)
4. Cregan P, Yamamoto A, Lum A, et al. Comparison of four methods for rapid detection of Pneumocystis carinii in respiratory specimens. J Clin Microbiol 1990;28:2432-6. (Pubitemid 20353516)
5. Zaman MK, Wooten OJ, Suprahmanya B, et al. Rapid noninvasive diagnosis of Pneumocystis carinii from induced liquefied sputum. Ann Intern Med 1988;109:7-10.
6. Azoulay E, Schlemmer B. Diagnostic strategy in cancer patients with acute respiratory failure. Intensive Care Med 2006;32:808-22. (Pubitemid 43781361)
7. Durand-Joly I, Chabé M, Soula F, et al. Molecular diagnosis of Pneumocystis pneumonia. FEMS Immunol Med Microbiol 2005;45: 405-10. (Pubitemid 41247776)
8. Graves DC, Chary-Reddy S, Becker-Hapak M. Detection of Pneumocystis carinii in induced sputa from immunocompromised patients using a repetitive DNA probe. Mol Cell Probes 1997;11:1-9. (Pubitemid 27117071)
9. Travis J, Hart E, Helm J, et al. Retrospective review of Pneumocystis jirovecii pneumonia over two decades. Int J STD AIDS 2009;20:200-1.
10. Sing A, Trebesius K, Roggenkamp A, et al. Evaluation of diagnostic value and epidemiological implications of PCR for Pneumocystis carinii in different immunosuppressed and immunocompetent patient groups. J Clin Microbiol 2000;38:1461-7. (Pubitemid 30216672)
11. Huang SN, Fischer SH, O'Shaughnessy E, et al. Development of a PCR assay for diagnosis of Pneumocystis carinii pneumonia based on amplification of the multicopy major surface glycoprotein gene family. Diagn Microbiol Infect Dis 1999;35:27-32. (Pubitemid 29435938)
12. Chen YC, Eisner JD, Kattar MM, et al. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J Clin Microbiol 2001;39:4042-51. (Pubitemid 33029781)
13. Loebstein R, Mahagna R, Maor Y, et al. Hepatitis C, B, and human immunodeficiency virus infections in illicit drug users in Israel: Prevalence and risk factors. Isr Med Assoc J 2008;10:775-8.
14. Lipschik GY, Gill VJ, Lundgren JD, et al. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 1992;340:203-6.
15. Leibovitz E, Pollack H, Moore T, et al. Comparison of PCR and standard cytological staining for detection of Pneumocystis carinii from respiratory specimens from patients with or at high risk for infection by human immunodeficiency virus. J Clin Microbiol 1995;33:3004-7.
16. Morris A, Wei K, Afshar K, et al. Epidemiology and clinical significance of pneumocystis colonization. J Infect Dis 2008;197:10-7. (Pubitemid 351263460)
17. Kasolo F, Lishimpi K, Chintu C, et al. Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses. J Pediatr 2002;140: 367-9. (Pubitemid 34289716)
18. Azoulay E, Bergeron A, Chevret S, et al. Polymerase chain reaction for diagnosing Pneumocystis pneumonia in non-HIV immunocompromised patients with pulmonary infiltrates. Chest 2009;135:655-61.
19. Flori P, Bellete B, Durand F, et al. Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jirovecii pneumonia from bronchoalveolar lavage specimens. J Med Microbiol 2004;53:603-7. (Pubitemid 38932356)
20. White DA, Wong PW, Downey R. The utility of open lung biopsy in patients with hematologic malignancies. Am J Respir Crit Care Med 2000;161:723-9. (Pubitemid 30171723)