Rapid mRNA-display selection of an IL-6 inhibitor using continuous-flow magnetic separation

Angewandte Chemie - International Edition

Volumn 50, Issue 36, 2011, Pages 8295-8298

  Olson, C Anders ^b   Adams, Jonathan D ^c   Takahashi, Terry T ^d   Qi, Hangfei ^b   Howell, Shannon M ^d   Wu, Ting Ting ^b   Roberts, Richard W ^d   Sun, Ren ^b   Soh, H Tom ^a  

^a University of California   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

antibodies; directed evolution; ligand design; mRNA; selection methods

Indexed keywords

AFFINITY REAGENTS; CONTINUOUS-FLOW; CONVENTIONAL METHODS; DIRECTED EVOLUTION; INTERLEUKIN-6; LIGAND DESIGN; MICROFLUIDICS TECHNOLOGY; MRNA; MRNA-DISPLAY; PROTEIN TARGETS; SELECTION METHODS;

MAGNETIC SEPARATION;

IMMUNOLOGY;

ANTIBODY; INTERLEUKIN 6; LIGAND; MESSENGER RNA;

AMINO ACID SEQUENCE; ARTICLE; DIRECTED MOLECULAR EVOLUTION; DRUG ANTAGONISM; GENETICS; HUMAN; IMMUNOLOGY; IMMUNOMAGNETIC SEPARATION; METABOLISM; MICROFLUIDICS; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; TUMOR CELL LINE;

AMINO ACID SEQUENCE; ANTIBODIES; CELL LINE, TUMOR; DIRECTED MOLECULAR EVOLUTION; HUMANS; IMMUNOMAGNETIC SEPARATION; INTERLEUKIN-6; LIGANDS; MICROFLUIDICS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN BINDING; RNA, MESSENGER;

EID: 80052079213     PISSN: 14337851     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.201101149     Document Type: Article

References (21)

1. D. H. Margulies, J. Immunol. 2005, 174, 2451.
2. O. Stoevesandt, M. J. Taussig, Proteomics 2007, 7, 2738.
3. H. K. Binz, P. Amstutz, A. Pluckthun, Nat. Biotechnol. 2005, 23, 1257.
4. A. Koide, C. W. Bailey, X. Huang, S. Koide, J. Mol. Biol. 1998, 284, 1141.
5. H. I. Liao, C. A. Olson, S. Hwang, H. Deng, E. Wong, R. S. Baric, R. W. Roberts, R. Sun, J. Biol. Chem. 2009, 284, 17512.
6. C. A. Olson, H. I. Liao, R. Sun, R. W. Roberts, ACS Chem. Biol. 2008, 3, 480.
7. L. Bloom, V. Calabro, Drug Discovery Today 2009, 14, 949.
8. F. N. Ishikawa, H. K. Chang, M. Curreli, H. I. Liao, C. A. Olson, P. C. Chen, R. Zhang, R. W. Roberts, R. Sun, R. J. Cote, M. E. Thompson, C. Zhou, ACS Nano 2009, 3, 1219.
9. A. M. Levin, G. A. Weiss, Mol. Biosyst. 2006, 2, 49.
10. R. W. Roberts, J. W. Szostak, Proc. Natl. Acad. Sci. USA 1997, 94, 12297.
11. T. T. Takahashi, R. W. Roberts, Methods Mol. Biol. 2009, 535, 293.
12. D. S. Wilson, A. D. Keefe, J. W. Szostak, Proc. Natl. Acad. Sci. USA 2001, 98, 3750.
13. B. Seelig, J. W. Szostak, Nature 2007, 448, 828.
14. Y. Liu, J. D. Adams, K. Turner, F. V. Cochran, S. S. Gambhir, H. T. Soh, Lab Chip 2009, 9, 1033.
15. G. S. Waldo, B. M. Standish, J. Berendzen, T. C. Terwilliger, Nat. Biotechnol. 1999, 17, 691.
16. D. L. Minor, Jr. , P. S. Kim, Nature 1994, 367, 660.
17. C. K. Smith, J. M. Withka, L. Regan, Biochemistry 1994, 33, 5510.
18. E. Cota, A. Steward, S. B. Fowler, J. Clarke, J. Mol. Biol. 2001, 305, 1185.
19. T. Taga, T. Kishimoto, Annu. Rev. Immunol. 1997, 15, 797.
20. S. Rose-John, J. Scheller, G. Elson, S. A. Jones, J. Leukocyte Biol. 2006, 80, 227.
21. F. L. Rock, X. Li, P. Chong, N. Ida, M. Klein, Biochemistry 1994, 33, 5146.