Metabolic engineering in strawberry fruit uncovers a dormant biosynthetic pathway

Metabolic Engineering

Volumn 13, Issue 5, 2011, Pages 527-531

  Hoffmann, Thomas ^a   Kurtzer, Robert ^a   Skowranek, Kilian ^a   Kießling, Peter ^a   Fridman, Eyal ^b   Pichersky, Eran ^c   Schwab, Wilfried ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Chalcone synthase; Eugenol synthase; Flavonoids; Fragaria ananassa; Isoeugenol synthase; Phenylpropanoids

Indexed keywords

CHALCONE SYNTHASE; FRAGARIA; ISOEUGENOL; PHENYLPROPANOIDS; SYNTHASES;

BIOCHEMISTRY; BIOSYNTHESIS; GENES; GENETIC ENGINEERING; ISOMERS; METABOLISM; ODORS;

FRUITS;

CHALCONE SYNTHASE; EUGENOL; FLAVONOID; ISOEUGENOL; ISOEUGENOL SYNTHASE; PHENYLPROPENE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; DOWN REGULATION; GENE SILENCING; METABOLIC ENGINEERING; NUCLEOTIDE SEQUENCE; ODOR; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; STRAWBERRY;

ACYLTRANSFERASES; DOWN-REGULATION; FLAVONOIDS; FRAGARIA; FRUIT; GENE SILENCING; PLANTS, GENETICALLY MODIFIED;

FRAGARIA VESCA; FRAGARIA X ANANASSA;

EID: 80052031070     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2011.06.002     Document Type: Article

References (22)

1. Bassil N.V., Gunn M., Folta K., Lewers K. Microsatellite markers for Fragaria from 'Strawberry Festival' expressed sequence tags. Molecular Ecology Notes 2006, 6:473-476.
2. Escudero A., Campo E., Fariña L., Cacho J., Ferreira V. Analytical characterization of five premium red wines. Insights into the role of odor families and the concept of fruitiness of wines. Journal of Agricultural and Food Chemistry 2007, 55:4501-4510.
3. Flamini R., Dalla Vedova A., Cancian D., Panighel A., De Rosso M. GC/MS-positive ion chemical ionization and MS/MS study of volatile benzene compounds in five different woods used in barrel making. Journal of Mass Spectrometry 2007, 42:641-646.
4. Hirvi T., Honkanen E. The volatiles of two new strawberry cultivars, "Annelie" and "Alaska Pioneer", obtained by backcrossing of cultivated strawberries with wild strawberries, Fragaria vesca, Rügen and Fragaria virginiana. Zeitschrift für Lebensmittel-Untersuchung und Forschung 1982, 175:113-116.
5. Hoffmann T., Kalinowski G., Schwab W. RNAi-induced silencing of gene expression in strawberry fruit (Fragaria×ananassa) by agroinfiltration: a rapid assay for gene function analysis. The Plant Journal 2006, 48:818-826.
6. Jefferson A.R. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter 1987, 5:387-405.
7. Koeduka T., Fridman E., Gang D.R., Vassão D.G., Jackson B.L., Kish C.M., Orlova I., Spassova S.M., Lewis N.G., Noel J., Baiga T.J., Dudareva N., Pichersky E. Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester. Proceedings of the National Academy of Sciences 2006, 103:10128-10133.
8. Koeduka T., Louie G.V., Orlova I., Kish C.H., Ibdah M., Wilkerson C.G., Bowman M.E., Baiga T.J., Noel J.P., Dudareva N., Pichersky E. The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages. The Plant Journal 2008, 54:362-374.
9. Lazo G.R., Pascal A.S., Ludwig R.A. A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology 1991, 9:963-967.
10. Lopez-Gomez R., Gomez-Lim M.A. A method for extraction intact RNA from fruits rich in polysaccharides using ripe mango mesocarp. HortScience 1992, 27:440-442.
11. Louie G.V., Baiga T.J., Bowman M.E., Koeduka T., Taylor J.H., Spassova S.M., Pichersky E., Noel J.P. Structure and reaction mechanism of basil eugenol synthase. PLoS ONE 2007, 10(e993):1-12.
12. Lunkenbein S., Coiner H., Ric de Vos C.H., Schaart J.G., Boone M.J., Krens F.A., Schwab W., Salentijn E.M.J. Molecular characterization of a stable antisense chalcone synthase phenotype in strawberry (Fragaria×ananassa). Journal of Agricultural and Food Chemistry 2006, 54:2145-2153.
13. Osorio S., Castillejo C., Quesada M.A., Medina-Escobar N, Brownsey G., Suau R., Heredia A., Botella M.A., Valpuesta V. Partial demethylation of oligogalacturonides by pectin methyl esterase 1 is required for eliciting defense responses in wild strawberry (Fragaria vesca). The Plant Journal 2008, 54:43-55.
14. Pyysalo T., Honkanen E., Hirvi T. Volatiles of wild strawberries, Fragaria vesca L., compared to those of cultivated berries, Fragaria×ananassa cv. Senga sengana. Journal of Agricultural and Food Chemistry 1979, 27:19-22.
15. Raab T., López-Ráez J.A., Klein D., Caballero J.L., Moyano E., Schwab W., Muñoz-Blanco J. FaQR, required for the biosynthesis of the strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone, encodes an enone oxidoreductase. Plant Cell 2006, 18:1023-1037.
16. Shulaev V., Sargent D.J., Crowhurst R.N., Mockler T.C., Folkerts O., Delcher A.L., Jaiswal P., Mockaitis K., Liston A., Mane S.P., Burns P., Davis T.M., Slovin J.P., Bassil N., Hellens R.P., Evans C., Harkins T., Kodira C., Desany B., Crasta O.R., Jensen R.V., Allan A.C., Michael T.P., Setubal J.C., Celton J.M., Rees D.J.G., Williams K.P., Holt S.H., Ruiz Rojas J.J., Chatterjee M., Liu B., Silva H., Meisel L., Adato A., Filichkin S.A., Troggio M., Viola R., Lynn Ashman T., Wang H., Dharmawardhana P., Elser J., Raja R., Priest H.D., Bryant D.W., Fox S.E., Givan S.A., Wilhelm L.J., Naithani S., Christoffels A., Salama D.Y., Carter J., Lopez Girona E., Zdepski A., Wang W., Kerstetter R.A., Schwab W., Korban S.S., Davik J., Monfort A., Denoyes-Rothan B., Arus P., Mittler R., Flinn B., Aharoni A., Bennetzen J.L., Salzberg S.L., Dickerman A.W., Velasco R., Borodovsky M., Veilleux R.E., Folta K.M. The genome of woodland strawberry (Fragaria vesca). Nature Genetics 2011, 43:109-118.
17. Ulrich D., Komes D., Olbricht K., Hoberg E. Diversity of aroma patterns in wild and cultivated Fragaria accessions. Genetic Resources and Crop Evolution 2007, 54:1185-1196.
18. Ulrich D., Rapp A., Hoberg E. Analysis of strawberry flavour-quantification of the volatile components of varieties of cultivated and wild strawberries. Zeitschrift Lebensmittel-Untersuchung und Forschung 1995, 200:217-220.
19. Vassão D.G., Gang D.R., Koeduka T., Jackson B., Pichersky E., Davin L.B., Lewis N.G. Chavicol formation in sweet basil (Ocimum basilicum): cleavage by an esterified C9 hydroxyl group with NAD(P)H-dependent reduction. Organic and Biomolecular Chemistry 2006, 4:2733-2744.
20. Vassão D.G., Kim S.J., Milhollan J.K., Eichinger D., Davin L.B., Lewis N.G. A pinoresinol-lariciresinol reductase homologue from the creosote bush (Larrea tridentate) catalyzes the efficient in vitro conversion of p-coumaryl/coniferyl alcohol esters into the allylphenyls chavicol/eugenol, but not the propenylphenols p-anol/isoeugenol. Archives of Biochemistry and Biophysics 2007, 465:209-218.
21. Zabetakis I., Holden M.A. Strawberry flavor: analysis and biosynthesis. Journal of the Science of Food and Agriculture 1997, 74:421-434.
22. Zuker A., Tzfira T., Ben-Meir H., Ovadis M., Shklarman E., Itzhaki H., Forkmann G., Martens S., Neta-Sharir I., Weiss D., Vainstein A. Modification of flower color and fragrance by antisense suppression of the flavanone 3-hydroxylase gene. Molecular Breeding 2002, 9:33-41.