Development of an all-in-one lentiviral vector system based on the original TetR for the easy generation of Tet-on cell lines

PLoS ONE

Volumn 6, Issue 8, 2011, Pages

  Benabdellah, Karim ^a   Cobo, Marién ^a   Muñoz, Pilar ^a   Toscano, Miguel G ^a   Martin, Francisco ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

5' NUCLEOTIDASE; ACTIVATED LEUKOCYTE CELL ADHESION MOLECULE; CD19 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; DOXYCYCLINE; ENDOGLIN; ENHANCED GREEN FLUORESCENT PROTEIN; HLA DR ANTIGEN; LENTIVIRUS VECTOR; REPRESSOR PROTEIN; TETR PROTEIN; THY 1 ANTIGEN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN;

ADIPOCYTE; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL LINE; CELL NUCLEUS; CONTROLLED STUDY; CYTOMEGALOVIRUS; GENE CASSETTE; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENOME; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOCYTOPLASMIC TRANSPORT; OPERON; OSTEOCYTE; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; REPRESSOR GENE; SPLEEN FOCUS FORMING VIRUS; TET ON SYSTEM; TETR REPRESSOR GENE; TRANSGENE; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; FIBROBLAST; GENE VECTOR; GENETIC PROCEDURES; GENETICS; LENTIVIRINAE; METABOLISM; METHODOLOGY; PROTEIN TRANSPORT;

SPLEEN FOCUS-FORMING VIRUS;

CELL CULTURE TECHNIQUES; CELL LINE; CELL NUCLEUS; CYTOMEGALOVIRUS; DOXYCYCLINE; FIBROBLASTS; GENE EXPRESSION; GENETIC TECHNIQUES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; MESENCHYMAL STEM CELLS; PROMOTER REGIONS, GENETIC; PROTEIN TRANSPORT; REPRESSOR PROTEINS; TRANSGENES;

EID: 80051900035     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0023734     Document Type: Article

References (29)

1. Gossen M, Bujard H, (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A 89: 5547-5551.
2. Toniatti C, Bujard H, Cortese R, Ciliberto G, (2004) Gene therapy progress and prospects: transcription regulatory systems. Gene Ther 11: 649-657.
3. Wells KD, Foster JA, Moore K, Pursel VG, Wall RJ, (1999) Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch. Transgenic Res 8: 371-381.
4. Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, et al. (2000) Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci U S A 97: 7963-7968.
5. Vieyra DS, Goodell MA, (2007) Pluripotentiality and conditional transgene regulation in human embryonic stem cells expressing insulated tetracycline-ON transactivator. Stem Cells 25: 2559-2566.
6. Barde I, Zanta-Boussif MA, Paisant S, Leboeuf M, Rameau P, et al. (2005) Efficient control of gene expression in the hematopoietic system using a single Tet-on inducible lentiviral vector. Mol Ther.
7. Judelson HS, Narayan R, Fong AM, Tani S, Kim KS, (2007) Performance of a tetracycline-responsive transactivator system for regulating transgenes in the oomycete Phytophthora infestans. Curr Genet 51: 297-307.
8. Sisson TH, Hansen JM, Shah M, Hanson KE, Du M, et al. (2006) Expression of the reverse tetracycline-transactivator gene causes emphysema-like changes in mice. Am J Respir Cell Mol Biol 34: 552-560.
9. Perl AK, Zhang L, Whitsett JA, (2009) Conditional expression of genes in the respiratory epithelium in transgenic mice: cautionary notes and toward building a better mouse trap. Am J Respir Cell Mol Biol 40: 1-3.
10. Whitsett JA, Perl AK, (2006) Conditional control of gene expression in the respiratory epithelium: A cautionary note. Am J Respir Cell Mol Biol 34: 519-520.
11. Morimoto M, Kopan R, (2009) RtTA toxicity limits the usefulness of the SP-C-rtTA transgenic mouse. Dev Biol 325: 171-178.
12. Yao F, Svensjo T, Winkler T, Lu M, Eriksson C, et al. (1998) Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum Gene Ther 9: 1939-1950.
13. Nghiem P, Park PK, Kim Y, Vaziri C, Schreiber SL, (2001) ATR inhibition selectively sensitizes G1 checkpoint-deficient cells to lethal premature chromatin condensation. Proc Natl Acad Sci U S A 98: 9092-9097.
14. Trapani JG, Korn SJ, (2003) Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines. BMC Neurosci 4: 15.
15. Reeves PJ, Callewaert N, Contreras R, Khorana HG, (2002) Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc Natl Acad Sci U S A 99: 13419-13424.
16. van de Wetering M, Oving I, Muncan V, Pon Fong MT, Brantjes H, et al. (2003) Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector. EMBO Rep 4: 609-615.
17. Wiederschain D, Wee S, Chen L, Loo A, Yang G, et al. (2009) Single-vector inducible lentiviral RNAi system for oncology target validation. Cell Cycle 8: 498-504.
18. Abu-Hamad S, Sivan S, Shoshan-Barmatz V, (2006) The expression level of the voltage-dependent anion channel controls life and death of the cell. Proc Natl Acad Sci U S A 103: 5787-5792.
19. Yao F, Theopold C, Hoeller D, Bleiziffer O, Lu Z, (2006) Highly efficient regulation of gene expression by tetracycline in a replication-defective herpes simplex viral vector. Mol Ther 13: 1133-1141.
20. Ogueta SB, Yao F, Marasco WA, (2001) Design and in vitro characterization of a single regulatory module for efficient control of gene expression in both plasmid DNA and a self-inactivating lentiviral vector. Mol Med 7: 569-579.
21. Cockrell AS, Kafri T, (2007) Gene delivery by lentivirus vectors. Mol Biotechnol 36: 184-204.
22. Escors D, Breckpot K, (2010) Lentiviral vectors in gene therapy: their current status and future potential. Arch Immunol Ther Exp (Warsz) 58: 107-119.
23. Kafri T, van Praag H, Gage FH, Verma IM, (2000) Lentiviral vectors: regulated gene expression. Mol Ther 1: 516-521.
24. Licursi M, Christian SL, Pongnopparat T, Hirasawa K, (2011) In vitro and in vivo comparison of viral and cellular internal ribosome entry sites for bicistronic vector expression. Gene Ther 18: 631-636.
25. Demaison C, Parsley K, Brouns G, Scherr M, Battmer K, et al. (2002) High-level transduction and gene expression in hematopoietic repopulating cells using a human immunodeficiency [correction of imunodeficiency] virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter. Hum Gene Ther 13: 803-813.
26. Haack K, Cockrell AS, Ma H, Israeli D, Ho SN, et al. (2004) Transactivator and structurally optimized inducible lentiviral vectors. Mol Ther 10: 585-596.
27. Toscano MG, Benabdellah K, Munoz P, Frecha C, Cobo M, et al. (2009) Was cDNA sequences modulate transgene expression of was promoter-driven lentiviral vectors. Hum Gene Ther 20: 1279-1290.
28. Toscano MG, Frecha C, Ortega C, Santamaria M, Martin F, et al. (2004) Efficient lentiviral transduction of Herpesvirus saimiri immortalized T cells as a model for gene therapy in primary immunodeficiencies. Gene Ther 11: 956-961.
29. Rodriguez R, Meuth M, (2006) Chk1 and p21 cooperate to prevent apoptosis during DNA replication fork stress. Mol Biol Cell 17: 402-412.