A fluorescent assay to quantitatively measure in vitro acyl CoA:diacylglycerol acyltransferase activity

Journal of Lipid Research

Volumn 52, Issue 9, 2011, Pages 1760-1764

  McFie, Pamela J ^a   Stone, Scot J ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Lipid synthesis; Radioactivity; Triacylglycerol

Indexed keywords

[N [(7 NITRO 2 1,3 BENZOXADIAZOL 4 YL)METHYL]AMINO]PALMITOYL COENZYME A; [N [(7 NITRO 2 1,3 BENZOXADIAZOL 4 YL)METHYL]AMINO]TRIACYLGLYCEROL; ACYL COENZYME A; DIACYLGLYCEROL ACYLTRANSFERASE; PALMITOYL COENZYME A; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; IMAGING SYSTEM; IN VITRO STUDY; INTERMETHOD COMPARISON; ISOTOPE LABELING; MOLECULAR IMAGING; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; QUANTITATIVE STUDY; RADIOACTIVITY; SYSTEM ANALYSIS;

ACYL COENZYME A; ANIMALS; BIOLOGICAL ASSAY; DIACYLGLYCEROL O-ACYLTRANSFERASE; FLUORESCENT DYES; HEK293 CELLS; HUMANS; MOLECULAR STRUCTURE; REPRODUCIBILITY OF RESULTS; TRIGLYCERIDES;

EID: 80051818920     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D016626     Document Type: Article

References (17)

1. Unger, R H, and Y T Zhou. 2001. Lipotoxicity of beta-cells in obesity and in other causes of fatty acid spillover. Diabetes. 50 (Suppl. 1): S118-S121. (Pubitemid 32148232)
2. Unger, R H, and L. Orci. 2002. Lipoapoptosis: Its mechanism and its diseases. Biochim. Biophys. Acta. 1585: 202-212. (Pubitemid 36092084)
3. Rosenbaum, M., R L Leibel, and J. Hirsch. 1997. Obesity. N. Engl. J. Med. 337: 396-406.
4. Kennedy, E P 1957. Metabolism of lipides. Annu. Rev. Biochem. 26: 119-148.
5. Cases, S., S J Smith, Y W Zheng, H M Myers, S R Lear, E. Sande, S. Novak, C. Collins, C B Welch, A J Lusis, et al. 1998. Identification of a gene encoding an acyl CoA: Diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad. Sci. USA. 95: 13018-13023. (Pubitemid 28509516)
6. Cases, S., S J Stone, P. Zhou, E. Yen, B. Tow, K D Lardizabal, T. Voelker, and R V Farese, Jr. 2001. Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members. J. Biol. Chem. 276: 38870-38876.
7. Stone, S J, H M Myers, S M Watkins, B E Brown, K R Feingold, P M Elias, and R V Farese, Jr. 2004. Lipopenia and skin barrier abnormalities in DGAT2-deficient mice. J. Biol. Chem. 279: 11767-11776. (Pubitemid 38401682)
8. Smith, S J, S. Cases, D R Jensen, H C Chen, E. Sande, B. Tow, D A Sanan, J. Raber, R H Eckel, and R V Farese, Jr. 2000. Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT. Nat. Genet. 25: 87-90. (Pubitemid 30257043)
9. Yen, C L, M. Monetti, B J Burri, and R V Farese, Jr. 2005. The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters. J. Lipid Res. 46: 1502-1511. (Pubitemid 43109815)
10. Coleman, R A 1992. Diacylglycerol acyltransferase and monoacylglycerol acyltransferase from liver and intestine. Methods Enzymol. 209: 98-104.
11. Coleman, R, and R M Bell. 1976. Triacylglycerol synthesis in isolated fat cells. Studies on the microsomal diacylglycerol acyltransferase activity using ethanol-dispersed diacylglycerols. J. Biol. Chem. 251: 4537-4543.
12. McFie, P J, S L Stone, S L Banman, and S J Stone. 2010. Topological orientation of acyl-CoA: Diacylglycerol acyltransferase-1 (DGAT1) and identification of a putative active site histidine and the role of the N terminus in dimer/tetramer formation. J. Biol. Chem. 285: 37377-37387.
13. Brasaemle, D L, T. Barber, N E Wolins, G. Serrero, E J Blanchette-Mackie, and C. Londos. 1997. Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein. J. Lipid Res. 38: 2249-2263. (Pubitemid 27495384)
14. Lehner, R, and A. Kuksis. 1993. Purification of an acyl-CoA hydrolase from rat intestinal microsomes. A candidate acyl-enzyme intermediate in glycerolipid acylation. J. Biol. Chem. 268: 24726-24733. (Pubitemid 23335483)
15. Harris, C A, J T Haas, R S Streeper, S J Stone, M. Kumari, K. Yang, X. Han, N. Brownell, R W Gross, R. Zechner, et al. 2011. DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes. J. Lipid Res. 52: 657-667.
16. Meegalla, R L, J T Billheimer, and D. Cheng. 2002. Concerted elevation of acyl-coenzyme A: Diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin. Biochem. Biophys. Res. Commun. 298: 317-323. (Pubitemid 35333707)
17. Shih, M Y, M A Kane, P. Zhou, C L Yen, R S Streeper, J L Napoli, and R V Farese, Jr. 2009. Retinol esterification by DGAT1 is essential for retinoid homeostasis in murine skin. J. Biol. Chem. 284: 4292-4299.