Directing human embryonic stem cell differentiation by non-viral delivery of siRNA in 3D culture

Biomaterials

Volumn 32, Issue 31, 2011, Pages 7793-7800

  Zoldan, Janet ^a   Lytton Jean, Abigail K R ^a   Karagiannis, Emmanouil D ^a   Deiorio Haggar, Kaila ^a   Bellan, Leon M ^a   Langer, Robert ^a,b   Anderson, Daniel G ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b USA   (United States)

Author keywords

3D culture; Differentiation; Gene therapy; Human embryonic stem cells; KDR; SiRNA delivery

Indexed keywords

3D CULTURE; 3D SCAFFOLDS; CELL TYPES; DOWN-REGULATION; EFFICIENT SYSTEMS; GFP EXPRESSION; HUMAN EMBRYONIC STEM CELLS; KDR; MATRIX; NONVIRAL; RECEPTOR GENES; REGENERATIVE MEDICINE; SIRNA DELIVERY; SIRNA SILENCING; STEM CELL DIFFERENTIATION; UP-REGULATION;

CELL GROWTH; GENES; GENETIC ENGINEERING; RNA; STEM CELLS; THREE DIMENSIONAL;

CELL CULTURE;

LIPID; LIPIDOID; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; VASCULOTROPIN RECEPTOR 2;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; DOWN REGULATION; EMBRYONIC STEM CELL; ENDODERM; GENE EXPRESSION REGULATION; GENE SILENCING; GROWTH INHIBITION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MESODERM; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; UPREGULATION;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELLS, CULTURED; EMBRYOID BODIES; EMBRYONIC STEM CELLS; GENE KNOCKDOWN TECHNIQUES; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HUMANS; MICE; PROTEIN INTERACTION MAPS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; VIRUSES;

EID: 80051815253     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2011.06.057     Document Type: Article

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