Small-molecule inhibitors of the Wnt pathway potently promote cardiomyocytes from human embryonic stem cell-derived mesoderm

Circulation Research

Volumn 109, Issue 4, 2011, Pages 360-364

  Willems, Erik ^a,b   Spiering, Sean ^a   Davidovics, Herman ^a   Lanier, Marion ^b,c   Xia, Zebin ^a   Dawson, Marcia ^a   Cashman, John ^b,c   Mercola, Mark ^a,b  

^a BURNHAM INSTITUTE   (United States)

^b ChemRegen Inc   (United States)

^c HUMAN BIOMOLECULAR RESEARCH INSTITUTE   (United States)

Author keywords

cardiogenesis; human embryonic stem cells; small molecules; Wnt inhibitors

Indexed keywords

CELL PROTEIN; RED FLUORESCENT PROTEIN; WNT PROTEIN;

ARTICLE; CELL DIFFERENTIATION; CELL LINEAGE; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE; GENE EXPRESSION PROFILING; GENETIC TRANSCRIPTION; HEART DEVELOPMENT; HEART MUSCLE CELL; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MESODERM; MOLECULAR LIBRARY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; SIGNAL TRANSDUCTION;

CARDIAC MYOSINS; CELL DIFFERENTIATION; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DISCOVERY; EMBRYONIC STEM CELLS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUMINESCENT PROTEINS; MESODERM; MICROSCOPY, FLUORESCENCE; MYOCYTES, CARDIAC; MYOSIN HEAVY CHAINS; PROMOTER REGIONS, GENETIC; SIGNAL TRANSDUCTION; SMALL MOLECULE LIBRARIES; TIME FACTORS; TRANSFECTION; WNT PROTEINS;

EID: 80051796581     PISSN: 00097330     EISSN: 15244571     Source Type: Journal    
DOI: 10.1161/CIRCRESAHA.111.249540     Document Type: Article

References (8)

1. Segers VF, Lee RT. Stem-cell therapy for cardiac disease. Nature. 2008;451:937-942. (Pubitemid 351301749)
2. Willems E, Lanier M, Forte E, Lo F, Cashman J, Mercola M. A chemical biology approach to myocardial regeneration. J Cardiovasc Transl Res. 2011;4:340-350.
3. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS One. 2009;4:e5046.
4. ten Berge D, Koole W, Fuerer C, Fish M, Eroglu E, Nusse R. Wnt signaling mediates self-organization and axis formation in embryoid bodies. Cell Stem Cell. 2008;3:508-518.
5. Kattman SJ, Witty AD, Gagliardi M, Dubois NC, Niapour M, Hotta A, Ellis J, Keller G. Stage-specific optimization of activin/nodal and bmp signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. Cell Stem Cell. 2011;8:228-240.
6. Chen B, Dodge ME, Tang W, Lu J, Ma Z, Fan CW, Wei S, Hao W, Kilgore J, Williams NS, Roth MG, Amatruda JF, Chen C, Lum L. Small moleculemediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat Chem Biol. 2009;5:100-107.
7. Wang H, Hao J, Hong CC. Cardiac induction of embryonic stem cells by a small molecule inhibitor of Wnt/beta-catenin signaling. ACS Chem Biol. 2011;6:192-197.
8. Foley AC, Mercola M. Heart induction by Wnt antagonists depends on the homeodomain transcription factor Hex. Genes Dev. 2005;19:387-396. (Pubitemid 40189301)