Detection of predation using qPCR: Effect of prey quantity, elapsed time, chaser diet, and sample preservation on detectable quantity of prey DNA

Journal of Insect Science

Volumn 9, Issue , 2009, Pages

  Weber, Donald C ^a   Lundgren, Jonathan G ^b  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b NORTH CENTRAL AGRICULTURAL RESEARCH LABORATORY   (United States)

Author keywords

Biological control; Chrysomelidae; Coccinellidae; Coleomegilla; Colorado potato beetle; Generalist predators; Gut analysis; Ladybeetle; Leaf beetle; Leptinotarsa; Molecular ecology

Indexed keywords

APHIDIDAE; CHRYSOMELIDAE; COCCINELLIDAE; COLEOMEGILLA; COLEOMEGILLA MACULATA; COLEOPTERA; LEPTINOTARSA; LEPTINOTARSA DECEMLINEATA; SOLANUM TUBEROSUM;

DNA;

ANIMAL; ARTICLE; BEETLE; DIET; GENETICS; METHODOLOGY; OOCYTE; PHYSIOLOGY; POLYMERASE CHAIN REACTION; PREDATION; TIME;

ANIMALS; BEETLES; DIET; DNA; OVUM; POLYMERASE CHAIN REACTION; PREDATORY BEHAVIOR; TIME FACTORS;

EID: 68449093407     PISSN: None     EISSN: 15362442     Source Type: Journal    
DOI: 10.1673/031.009.4101     Document Type: Article

References (34)

1. Calder CR, Harwood JD, Symondson WOC. 2005. Detection of scavenged material in the guts of predators using monoclonal antibodies: A significant source of error in measurement of predation? Bulletin of Entomological Research 95: 57-62.
2. Chen Y, Giles KL, Payton ME, Greenstone MH. 2000. Identifying key cereal aphid predators by molecular gut analysis. Molecular Ecology 9: 1887-1898.
3. Deagle BE, Eveson JP, Jarman SN. 2006. Quantification of damage in DNA recovered from highly degraded samples - A case study on DNA in faeces. Frontiers in Zoology 3: 11
4. Fournier V, Hagler JR, Daane KM, de León JH, Groves RL, Costa HS, Henneberry TJ. 2006. Development and application of a glassy-winged sharpshooter and smoke-tree sharpshooter egg-specific predator gut content ELISA. Biological Control 37: 108-118.
5. Greenstone MH, Hunt JH. 1993. Determination of prey antigen half-life in Polistes metricus using a monoclonal antibody-based immun-odot assay. Entomologia experimentalis et applicata 68: 1-7.
6. Greenstone MH, Rowley DL, Weber DC, Payton ME, Hawthorne DJ. 2007. Feeding mode and prey detectability half-lives in molecular gut-content analysis: An example with two predators of the Colorado potato beetle. Bulletin of Entomological Research 97: 201-209.
7. Hagler JR. 1998. Variation in the efficacy of several predator gut content immunoassays. Biological Control 12: 25-32.
8. Hagler JR, Naranjo SE. 1997. Measuring the sensitivity of an indirect predator gut content ELISA: Detectability of prey remains in relation to predator species, temperature, time, and meal size. Biological Control 9: 112-119.
9. Harper GL, Sheppard SK, Harwood JD, Read DS, Glen DM, Bruford MW, Symondson WOC. 2006. Evaluation of temperature gradient gel electrophoresis for the analysis of prey DNA within the guts of invertebrate predators. Bulletin of Entomological Research 96: 295-304.
10. Harwood JD, Bostrom MR, Hladilek EE, Wise DH, Obrycki JJ. 2007. An order-specific monoclonal antibody to Diptera reveals the impact of alternative prey on spider feeding behavior in a complex food web. Biological Control 41: 397-407.
11. Harwood JD, Obrycki JJ. 2005. Quantifying aphid predation rates of generalist predators in the field. European Journal of Entomology 102: 335-350.
12. Harwood JD, Phillips SW, Sunderland KD, Symondson WOC. 2001. Secondary predation: Quantification of food-chain errors in an aphid-spider-carabid system using monoclonal antibodies. Molecular Ecology 10: 2049-2057.
13. Hazzard RV, Ferro DN, van Driesche RG, Tuttle AF. 1991. Mortality of eggs of the Colorado potato beetle (Coleoptera: Chrysomelidae) from predation by Coleomegilla maculata (Coleoptera: Coccinellidae). Environmental Entomology 20: 841-848.
14. Hed BE, Windham MT, Grant JF. 1999. Survival of conidia of Discula destructiva in frass of the convergent lady beetle. Plant Disease 83: 806-809.
15. Hilbeck A, Eckel C, Kennedy GG. 1997. Predation on Colorado potato beetle eggs by generalist predators in research and commercial potato plantings. Biological Control 8: 191-196.
16. Hoogendoorn M, Heimpel GE. 2001. PCR-based gut content analysis of insect predators: Using ribosomal ITS-1 fragments from prey to estimate predation frequency. Molecular Ecology 10: 2059-2067.
17. Juen A, Traugott M. 2005. Detecting predation and scavenging by DNA gut-content analysis: A case study using a soil insect predator-prey system. Oecologia 142: 344-352.
18. Juen A, Traugott M. 2006. Amplification facilitators and multiplex PCR: Tools to overcome PCR-inhibition in DNA-gut-content analysis of soil-living invertebrates. Soil Biology and Biochemistry 38: 1872-1879.
19. Lövei GL, Monostori É, István A. 1985. Digestion rate in relation to starvation in the larva of a carabid predator, Poecilus cupreus. Ento-mologia experimentalis et applicata 37: 123-127.
20. Mager SR, Oomen MHA, Morente MM, Ratcliffe C, Knoxa K, Kerr DJ, Pezzella F, Riegman PHJ. 2007. Standard operating procedure for the collection of fresh frozen tissue samples. European Journal Of Cancer 43: 828-834.
21. Milliken GA, Johnson DE. 2002. Analysis of Messy Data, Volume III: Analysis of Covariance. Chapman and Hall.
22. Nejstgaard JC, Frischer ME, Simonelli P, Troedsson C, Brakel M, Adiyaman F, Sazhin AF, Artigas F. 2008. Quantitative PCR to estimate copepod feeding. Marine Biology 153: 565-577 .
23. SAS Institute 1998. StatView Version 5.0. SAS Institute, Inc, Cary, NC.
24. SAS Institute 2003. SAS/STAT version 9.1. SAS Institute, Inc, Cary, NC.
25. Sheppard SK, Bell JR, Sunderland KD, Fenlon J, Skirvin D, Symond-son WOC. 2005. Detection of secondary predation by PCR analyses of the gut contents of invertebrate generalist predators. Molecular Ecology 14: 4461-4468.
26. Sheppard SK, Harwood JD. 2005. Advances in molecular ecology: tracking trophic links through predator-prey food webs. Functional Ecology 19: 751-762.
27. Sopp PI, Sunderland KD. 1989. Some factors affecting the detection period of aphid remains in predators using ELISA. Entomologia ex-perimentalis et applicata 51: 11-20.
28. Sunderland KD. 1996. Progress in quantifying predation using antibody techniques. In: Symondson WOC, Liddell JE, editors. The Ecology of Agricultural Pests: Biochemical Approaches, pp. 419-455. Chapman and Hall.
29. Symondson WOC. 2002. Molecular identification of prey in predator diets. Molecular Ecology 11: 627-641.
30. Symondson WOC, Erickson ML, Liddell JE. 1999. Development of a monoclonal antibody for the detection and quantification of pred-ation on slugs within the Arion hortensis agg (Mollusca: Pulmonata). Biological Control 16: 274-282.
31. Symondson WOC, Liddell JE. 1995. Decay rates for slug antigens within the carabid predator Pterostichus melanarius monitored with a monoclonal antibody. Entomologia experimentalis et applicata 75: 245-250.
32. Symondson WOC, Sunderland KD, Greenstone MH. 2002. Can gen-eralist predators be effective biocontrol agents? Annual Review of Entomology 47: 561-594.
33. Troedsson C, Frischer ME, Nejstgaard JC, Thompson EM. 2007. Molecular quantification of differential ingestion and particle trapping rates by the appendicularian Oikopleura dioica as a function of prey size and shape. Limnology and Oceanography 52: 416-427.
34. Zhang GF, Lü ZC, Wan FH, Lövei GL. 2007. Real-time PCR quantification of Bemisia tabaci (Homoptera: Aleyrodidae) B-biotype remains in predator guts. Molecular Ecology Notes 7: 947-954.