Multiresistance, beta-lactamase-encoding genes and bacterial diversity in hospital wastewater in Rio de Janeiro, Brazil

Journal of Applied Microbiology

Volumn 111, Issue 3, 2011, Pages 572-581

  Chagas, T P G ^a   Seki, L M ^a   Cury, J C ^a   Oliveira, J A L ^a   Davila A M R ^a   Silva, D M ^a   Asensi, Marise D ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Bacterial diversity; Brazil; ESBL; Hospital wastewater; Multiresistance

Indexed keywords

ANTIBIOTICS; EFFLUENT TREATMENT; ESCHERICHIA COLI; GENES; HOSPITALS; SEWAGE PUMPING PLANTS; SEWAGE TREATMENT PLANTS; TANKS (CONTAINERS); WASTEWATER TREATMENT; WATER TREATMENT PLANTS;

BACTERIAL DIVERSITY; BRAZIL; EXTENDED-SPECTRUM BETA-LACTAMASE; HOSPITAL WASTEWATER; LACTAMASES; MICRO-ORGANISMS; MULTIRESISTANCE; SEWAGE-TREATMENT PLANTS; SPECTRA'S; WASTE WATER TREATMENT PLANTS;

EFFLUENTS;

CHLORINE; EXTENDED SPECTRUM BETA LACTAMASE;

ANTIBIOTIC RESISTANCE; BACTERIUM; CHLORINE; ENZYME ACTIVITY; GENE EXPRESSION; HOSPITAL SECTOR; PATHOGEN; POLLUTANT REMOVAL; SEWAGE; SPECIES DIVERSITY; WASTE FACILITY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM ISOLATION; BRAZIL; CONTROLLED STUDY; EFFLUENT; ENTEROBACTER CLOACAE; ESCHERICHIA COLI; GENETIC CODE; HOSPITAL; KLEBSIELLA PNEUMONIAE; MICROBIAL DIVERSITY; NONHUMAN; SEWAGE TREATMENT PLANT; TANK; WASTE WATER MANAGEMENT;

BRAZIL; RIO DE JANEIRO [BRAZIL];

BACTERIA (MICROORGANISMS); ENTEROBACTER CLOACAE; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; NEGIBACTERIA;

EID: 80051678328     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05072.x     Document Type: Article

References (53)

1. APHA. (1998) Standard Methods for the Examination Water and Wastewater, 20th edn. Washington, DC: American Public Health Association.
2. Arikan, B. and Aygan, A. (2009) Resistance variations of third generation of Cephalosporins in some of the Enterobacteriaceae members in hospital sewage. Int J Agric Biol 11, 93-96.
3. Ashelford, K.E., Chuzhanova, N.A., Fry, J.C., Jones, A.J. and Weightman, A.J. (2006) New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras. Appl Environ Microb 72, 5734-5741.
4. Baquero, F., Martínez, J.L. and Cantón, R. (2008) Antibiotics and antibiotic resistance in water environments. Curr Opin Biotechnol 19, 260-265.
5. Chao, A. (1987) Estimating the population size for capture-recapture data with unequal catchability. Biometrics 43, 783-791.
6. Chao, A. and Lee, S.M. (1992) Estimating the number of classes via sample coverage. J Am Stat Assoc 87, 210-217.
7. Clinical and Laboratory Standards Institute. (2009) Performance Standards for Antimicrobial Susceptibility Testing. CLSI/NCCLS M100-S19.Wayne, PA: Clinical and Laboratory Standards Institute.
8. Cole, J.R., Wang, Q., Cardenas, E., Fish, J., Chai, B., Farris, R.J., Kulam-Syed-Mohideen, A.S., McGarrell, D.M. et al. (2008) The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37, D141-D145.
9. Costanzo, S.D., Murby, J. and Bates, J. (2005) Ecosystem response to antibiotics entering the aquatic environmental. Mar Pollut Bull 51, 218-223.
10. DeSantis, T.Z., Hugenholtz, P., Keller, K., Brodie, E.L., Larsen, N., Piceno, Y.M., Phan, R. and Andersen, G.L. (2006) NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. Nucleic Acids Res 34, W394-W399.
11. Eisner, A., Fagan, E.J., Feierl, G., Kessler, H.H., Marth, E., Livermore, D.M. and Woodford, N. (2006) Emergence of Enterobacteriaceae isolates producing CTX-M extended spectrum beta-lactamase in Austria. Antimicrob Agents Chemother 50, 785-787.
12. Emmanuel, E., Perrodin, Y., Keck, G., Blanchard, J.M. and Vermande, P. (2005) Ecotoxicological risk assessment of hospital wastewater: a proposed framework for raw effluents discharging into urban sewer network. J Hazard Mater 117, 1-11.
13. Ewing, B. and Green, P. (1988) Base-calling of automated sequencer traces using Phred. II error probabilities. Genome Res 8, 186-194.
14. Felsenstein, J.P. (1993) Phylogenetic Inference Package Version 3.5c. Distributed by the author. Seattle: Department of Genetics, University of Washington.
15. Freitas, A.L.P., Machado, D.P., Soares, F.S.C. and Barth, A.L. (2003) Extended-Spectrum β-Lactamases in Klebsiella spp and Escherichia coli obtained in a Brazilian teaching hospital: detection, prevalence and molecular typing. Braz J Microbiol 34, 344-348.
16. Gallert, C., Fund, K. and Winter, J. (2005) Antibiotic resistance of bacteria in raw and biologically treated sewage and in groundwater below leaking sewers. Appl Microbiol Biotechnol 69, 106-112.
17. Galvin, S., Boyle, F., Hickey, P., Vellinga, A., Morris, D. and Martin Cormican, M. (2010) Enumeration and characterization of antimicrobial-resistant Escherichia coli bacteria in effluent from municipal, hospital, and secondary treatment facility sources. Appl Environ Microbiol 76, 4772-4779.
18. Good, I.J. (1953) The population frequencies of species and the estimation of the population parameters. Biometrika 40, 237-264.
19. Good, I.J. and Toulmin, G. (1956) The number of new species and the increase of population coverage when a sample is increased. Biometrika 43, 45-63.
20. Halling-Sorensen, B., Nors Nielsen, S., Lanzky, P.F., Ingerslev, F., Holten Lützheft, H.C. and Jorgensen, S.E. (1998) Occurrence, fate and effects of pharmaceutical substances in the environment - a review. Chemosphere 36, 357-393.
21. Hasman, H., Mevius, D., Veldman, K., Olesen, I. and Aarestrup, F.M. (2005) β-lactamases among extended-spectrum β-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in the Netherlands. J Antimicrob Chemother 56, 115-121.
22. Heuer, H., Krögerrecklenfort, E., Wellington, E.M.H., Egan, S., van Elsas, J.D., van Overbeek, L., Collard, J.M., Guillaume, G. et al. (2002) Gentamicin resistance genes in environmental bacteria: prevalence and transfer. FEMS Microbiol Ecol 42, 289-302.
23. Jarlier, V., Nicolas, M.H., Fournier, G. and Philippon, A. (1988) Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis 10, 867-878.
24. Jukes, T.H. and Cantor, C.R. (1969) Evolution of protein molecules. In Mammalian Protein Metabolism ed. Munro, H.N. pp. 21-132. New York: Academic.
25. Kiratisin, P., Apisarnthanarak, A., Laesripa, C. and Saifon, P. (2008) Molecular characterization and epidemiology of extended spectrum-beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. Antimicrob Agents Chemother 52, 2818-2824.
26. Macauley, J.J., Quiang, Z., Adams, C.D., Surampalli, R. and Mormile, M.R. (2006) Disinfection of swine wastewater using chlorine, ultraviolet light and ozone. Water Res 10, 2017-2026.
27. Marchesi, J.R., Takuichi, S., Weightman, A.J., Martin, T.A., Fry, J.C., Hiom, S.J. and Wade, W.G. (1998) Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64, 795-799.
28. Meirelles-Pereira, F., Pereira, A.M.S., Silva, M.C.G., Gonçalves, V.D., Brum, P.R., Castro, E.A.R., Pereira, A.A. and Pereira, J.A.A. (2002) Ecological aspects of the antimicrobial resistance in bacteria of importance to human infections. Braz J Microbiol 33, 287-293.
29. Mesa, R.J., Blanc, V., Blanc, A.R., Cortés, P., González, J.J., Lavilla, S., Miró, L., Muniesa, M. et al. (2006) Extended-spectrum ß-lactamase-producing Enterobacteriaceae in different environments (humans, food, animal farms and sewage). J Antimicrob Chemother 58, 211-215.
30. Mulvey, M.R., Soule, G., Boyd, D., Demczuk, W., Ahmed, R., and the Multi-provincial Salmonella Typhimurium case control study group. (2003) Characterization of the first extended-spectrum beta-lactamase-producing Salmonella isolate identified in Canada. J Clin Microbiol 41, 460-462.
31. Murray, G.E., Tobin, R.S., Junkins, B. and Kushner, D.J. (1984) Effect of chlorination on antibiotic resistance profiles of sewage-related bacteria. Appl Environ Microbiol 48, 73-77.
32. Murray, P.R., Baron, E.J., Jorgensen, J.H., Pfaller, M.A. and Yolken, R.H. (2003) Manual of Clinical Microbiology, 8th edn. Washington, DC: ASM Press.
33. Oliveira, S.C. and von Sperling, M. (2007) Análise da confiabilidade de estações de tratamento de esgotos. Eng Sanit Ambient 12, 389-398.
34. Oliveira, C.F., dal Forno, N.L.F., Alves, I.A.A., Horta, J.A., Rieger, A. and Alves, S.H. (2009) Prevalência das famílias TEM, SHV e CTX-M de beta-lactamases de espectro estendido em Escherichia coli e Klebsiella spp no Hospital Universitário de Santa Maria, Estado do Rio Grande do Sul. Rev Soc Bras Med Trop 42, 556-560.
35. Øvreås, L., Forney, L., Daae, F.L. and Torsvik, V. (1997) Distribution of bacterioplankton in meromictic lake saelevannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Appl Environ Microbiol 63, 3367-3373.
36. Paterson, D.L. (2006) Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Med 119, S20-S28.
37. Paterson, D.L. and Bonomo, R.A. (2005) Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev 18, 657-686.
38. Prado, T., Pereira, W.C., Silva, D.M., Seki, L.M., Carvalho, A.P.D'.A. and Asensi, M.D. (2008) Detection of extended-spectrum β-lactamase-producing Klebsiella pneumoniae in effluents and sludge of a hospital sewage treatment plant. Lett Appl Microbiol 46, 136-141.
39. Pruden, A., Pei, R., Storteboom, H. and Carlson, K.H. (2006) Antibiotic resistance genes as emerging contaminants: studies in northern Colorado. Environ Sci Technol 40, 7445-7450.
40. Reinthaler, F.F., Posch, J., Feierl, G., Wust, G., Hass, D., Ruckenbauer, G., Mascher, F. and Marth, E. (2003) Antibiotic resistance of E. coli in sewage and sludge. Water Res 37, 1685-1690.
41. Reinthaler, F.F., Feierl, G., Galle, H., Haas, D., Leitner, E., Mascher, F., Melkes, A., Posch, J. et al. (2010) ESBL-producing E. coli in Austrian sewage sludge. Water Res 44, 1981-1985.
42. Rezaee, A., Ansari, M., Khavanin, A., Sabzali, A. and Aryan, M.M. (2005) Hospital wastewater treatment using an integrated anaerobic aerobic fixed film bioreactor. Am J Environ Sci 1, 259-263.
43. Sasaki, T., Hirai, I., Niki, M., Nakamura, T., Komalamisra, C., Maipanich, W., Kusolsuk, T., Sa-nguankiat, S. et al. (2010) High prevalence of CTX-M b-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand. J Antimicrob Chemother 65, 666-668.
44. Schloss, P.D. and Handerlsman, J. (2005) Introducting to DOTUR, a computer program for defining operacional taxonomic units and estimating species richness. Appl Environ Microbiol 71, 1501-1506.
45. Shannon, C.E. and Weaver, W. (1949) The mathematical Theory of Information. Chicago: University of Illinois Press.
46. Storteboom, H., Arabi, M., Davis, J.G., Crimi, B. and Pruden, A. (2010) Tracking antibiotic resistance genes in the South Platte River basin using molecular signatures of urban, agricultural, and pristine sources. Environ Sci Technol 44, 7397-7404.
47. Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 40. Mol Biol Evol 24, 1596-1599.
48. Tennstedt, T., Szczepanowski, R., Braun, S., Pühler, A. and Schlüter, A. (2003) Occurrence of integron-associated resistance gene cassetes located on antibiotic resistance plasmids isolated from a wastewater treatment plant. FEMS Microbiol Ecol 45, 239-252.
49. Thompson, J.D., Higgins, D.G. and Gbson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.
50. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882.
51. Tsakona, M., Anagnostopoulou, E. and Gidarakos, E. (2007) Hospital waste management and toxicity evaluation: a case study. Waste Manag 27, 912-920.
52. Vecchia, A.D., Thewes, M.R., Harb Naime, R. and Spilki, F.R. (2009) Diagnóstico sobre a situação do tratamento de esgoto hospitalar no Brasil. Rev Saúde Ambiente 10, 65-69.
53. Wang, Q., Garrity, G.M., Tiedje, J.M. and Cole, J.R. (2007) Naïve Bayesian classifier for rapd assignment of RNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73, 5261-5267.