Molecular Cloning, Modeling, and Site-Directed Mutagenesis of Type III Polyketide Synthase from Sargassum binderi (Phaeophyta)

Marine Biotechnology

Volumn 13, Issue 5, 2011, Pages 845-856

  Baharum, Hariyanti ^a   Morita, Hiroyuki ^b   Tomitsuka, Akifumi ^b   Lee, Fong Chin ^a   Ng, Kim Yong ^c   Rahim, Raha Abdul ^a   Abe, Ikuro ^b   Ho, Chai Ling ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b UNIVERSITY OF TOKYO   (Japan)

^c T BioVenture Sdn Bhd   (Malaysia)

Author keywords

Brown seaweed; Kinetics; Sargassum binderi; Site mutagenesis; Type III polyketide synthase

Indexed keywords

ACTIVE SITE; AMINO ACID SEQUENCE; BROWN SEAWEED; CATALYTIC RESIDUE; CATALYTIC TRIAD; CONFORMATIONAL CHANGE; MOLECULAR CLONING; POLYKETIDE SYNTHASES; POLYKETIDES; PROTEIN STRUCTURES; RECOMBINANT PROTEIN; SARGASSUM BINDERI; SEQUENCE SIMILARITY; SITE DIRECTED MUTAGENESIS; SITE MUTAGENESIS; SUBSTRATE SPECIFICITY; TYPE III POLYKETIDE SYNTHASE; WILD TYPES;

AMINO ACIDS; BINDING SITES; CLONING; MUTAGENESIS; PLANTS (BOTANY); SEAWEED;

KETONES;

POLYKETIDE SYNTHASE;

AMINO ACID; ARRAY; CATALYSIS; CLONAL GROWTH; ENZYME ACTIVITY; MACROALGA; METABOLITE; MOLECULAR ANALYSIS; MUTATION; NUMERICAL MODEL; OBSERVATIONAL METHOD; PROTEIN; RECOMBINATION; SEAWEED; WILD POPULATION;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; CLASSIFICATION; ENZYME ACTIVE SITE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PHYSIOLOGY; SARGASSUM; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, PLANT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; POLYKETIDE SYNTHASES; SARGASSUM;

BACTERIA (MICROORGANISMS); PHAEOPHYCEAE; SARGASSUM; SARGASSUM BINDERI;

EID: 80051672117     PISSN: 14362228     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10126-010-9344-5     Document Type: Article

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