Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H)

In Vitro Cellular and Developmental Biology - Plant

Volumn 47, Issue 4, 2011, Pages 516-524

  Kang, Y M ^a   Park, D J ^b   Min, J Y ^c   Song, H J ^b   Jeong, M J ^b   Kim, Y D ^d   Kang, S M ^e   Karigar, C S ^f   Choi, M S ^b  

^a United States Department of Agriculture ^*  (United States)

^b Gyeongsang National University   (South Korea)

^c Sancheong Oriental Medicinal Herb Institute   (South Korea)

^d Hadong gun   (South Korea)

^e Gyeongsangnam do Forest Environment Research Institute   (South Korea)

^f BANGALORE UNIVERSITY   (India)

Author keywords

Hairy roots; Hyosamine 6 hydroxylase (H6H); Phytohormones; Putrescine N methyl transferase (PMT); Scopolia parviflora; Tropane alkaloids

Indexed keywords

AGROBACTERIUM RHIZOGENES; SCOPOLIA; SCOPOLIA PARVIFLORA;

EID: 80051667392     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-011-9367-2     Document Type: Article

References (29)

1. Canel C.; Lopes M. I.; Whitmer S.; Fits L.; Pasquali G.; Heijden R. J.; Hoge H. C. Verpoorte R. Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus. Planta 20: 5414-5419; 1998. doi: 10. 1007/s004250050338.
2. Chilton M. D.; Tepfer D. A.; Petit A.; David C.; Casse D. F.; Tempe J. Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells. Nature 295: 432-434; 1982. doi: 10. 1038/295432a0.
3. Constabel F. Protoplast technology applied to metabolite production. In: Giles KL (ed) Plant Protoplasts. Int Rev CytolSuppl Academic Press; 1983: 209-217.
4. Cusido R.; Palazon J.; Pinol M. T.; Bonfill M.; Marales C. Datura metel: in vitro propagation of tropane alkaloids. Planta Med 65: 144-148; 1999.
5. Dixon R. A. Natural products and plant disease resistance. Nature 411: 843-847; 2001. doi: 10. 1038/35081178.
6. Gamborg O. L.; Miller R. A.; Ojimaki K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158; 1968.
7. Hashimoto T.; Yamada Y. Purification and characterization of hyoscyamine 6ß-hydroxylase from root cultures of Hyoscyamus niger. Eur J Biochem 164: 277-285; 1987.
8. Hu Z. B.; Du M. Hairy root and its application in plant genetic engineering. J Integ Plant Biol 48: 121-127; 2006.
9. Iranbakhsh A.; Oshaghi M. A.; Majd A. Distribution of atropine and scopolamine in different organs and stages of development in Datura stramonium L. (Solanaceae) structure and ultrastructure of biosynthesizing cells. Acta BiolosiaCracoviensia Series Botanica 48: 13-18; 2006.
10. Jung H. Y.; Kang M. J.; Kang Y. M.; Kim Y. D.; Yang J. K.; Jung Y. G.; Choi M. S. Selection of optimal biotic elicitor on tropane alkaloid production of hairy roots in Scopolia parviflora Nak. Kor J Medicinal CropSci 11: 358-63; 2003.
11. Jung H. Y.; Kang M. J.; Kang Y. M.; Yun D. J.; Bahk J. D.; Chung Y. G.; Choi M. S. Production of tropane alkaloids by optimum culture conditions and XAD resin on hairy root cultures from Scopolia parviflora Nak. Kor J BiotechnolBioeng 6: 525-530; 2002.
12. Kang S. M.; Jung H. Y.; Kang Y. M.; Yun D. J.; Bahk J. D.; Yang J. K.; Choi M. S. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora. Plant Sci 166: 745-751; 2004a.
13. Kang Y. M.; Lee O. S.; Jung H. Y.; Kang S. M.; Lee B. H.; Karigar C. S.; Prasad T.; Bahk J. D.; Choi M. S. Overexpression of hyoscyamine 6β-hydroxylase (h6h) gene and enhanced production of tropane alkaloids in Scopolia parviflora hairy root lines. J MicrobiolBiotechnol 15: 91-98; 2005.
14. Kang Y. M.; Min J. Y.; Moon H. S.; Karigar C. S.; Prasad D. T.; Lee C. H.; Choi M. S. Rapid in vitro adventitious shoot propagation of Scopolia parviflora through rhizome cultures for enhanced production of tropane alkaloids. Plant Cell Rep 23: 128-133; 2004b.
15. Kim ES. Master's Thesis: Molecular Characterization of Two Scopolamine-Producing Genes, PMT and H6H, Derived from Scopolia parviflora. Graduate School of the Gyeongsang National University, Jinju, South Korea; 2003.
16. Lee O. S.; Kang Y. M.; Jung H. Y.; Min J. Y.; Kang S. M.; Karigar S. C.; Prasad D. T.; Bahk J. D.; Choi M. S. Enhanced production of tropane alkaloids in Scopolia parviflora by introducing the PMT (putrescine N-methyltansferase) gene. In vitro Cell DevBiol Plant 41: 167-172; 2005.
17. Mersey B.; Culter A. G. Differential distribution of specific indole alkaloids in leaves of Catharanthus roseus. Canadian J Bot 64: 1039-1045; 1986.
18. Min J. Y.; Jung H. Y.; Kang S. M.; Kim Y. D.; Kang Y. M.; Park D. J.; Prasad D. T.; Choi M. S. Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. BioresourceTechnol 98: 1748-1753; 2007a.
19. Min J. Y.; Park D. J.; Jeong M. J.; Song H. J.; Kang S. M.; Kang Y. M.; Choi M. S. Selection of tropane alkaloids high-produing lines by single cell cloning of Hyoscyamus niger L. root cultures. J Korean Forest Society 98: 142-147; 2007b.
20. Mitsuhara I.; Ugaki M.; Hirochika H.; Ohshima M.; Murakami T.; Gotoh Y.; Katayose Y.; Nakamura S.; Honkura R.; Nishimiya S.; Ueno K.; Mochizuki A.; Tanimoto H.; Tsugawa H.; Otsuki Y.; Ohashi Y. Efficient promoter cassettes for enhanced expression of foreign genes in cotyledonous and monocotyledonous plants. Plant Cell Physiol 37: 49-59; 1996.
21. Moyano E.; Fornale S.; Palazon J.; Cusido R. M.; Bonfill M.; Morales C.; Pinol M. T. Effect of Agrobacterium rhizogenes T-DNA on alkaloid production in Solanaceae plants. Phytochemistry 52: 1287-1292; 1999.
22. Palazon J.; Ocana A. N.; Vazquez L. H.; Mirjalili M. H. Application of metabolic engineering to the production of scopolamine. Molecules 13: 1722-1742; 2008.
23. Rhodes M. J. C.; Parr A. J.; Giulietti A.; Aird E. L. H. Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures. Plant Cell Tissue Organ Cult 38: 143-151; 1994.
24. Rothe G.; Hachiya A.; Yamada Y.; Hashimoto T.; DraÈger B. Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase. J Exp Bot 54: 2065-2070; 2003.
25. Sambrook J.; Fritch E. F.; Maniahtis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Lab. Press, Cold Spring Harbor, NY, USA; 1998.
26. Sato F.; Hashimoto T.; Hachiya A.; Tamura K.; Choi K. B.; Morishige T.; Fujimoto H.; Yamada Y. Metabolic engineering of plant alkaloid biosynthesis. ProcNatlAcadSci USA 98: 367-372; 2001.
27. Spena A.; Schmulling T.; Koncz C.; Schell J. Independent and synergistic activity of rolA, B, and C loci in stimulating abnormal growth in plants. EMBO J 6: 3891-3899; 1987.
28. Ursula S. S.; Karl H. K.; Carl S. Energy-filtering transmission electromicroscopy in the elemental analysis of psedoexfoliative material. Curr EyeRes 22: 154-162; 2001.
29. Washida D.; Shimomura K.; Takido M.; Kitanaka S. Auxins affected ginsenoside production and growth of hairy roots in Panax hybrid. Biol Pharmaca Bull 27: 657-160; 2004.