Overexpression of hyoscyamine 6β-hydroxylase(h6h) gene and enhanced production of tropane alkaloids in Scopolia parviflora hairy root lines

Journal of Microbiology and Biotechnology

Volumn 15, Issue 1, 2005, Pages 91-98

  Kang, Young Min ^a   Lee, Ok Sun ^a   Jung, Hee Young ^a   Kang, Seung Mi ^a   Lee, Byung Hyun ^a   Karigar, Chandrakant ^b   Prasad, Theertha ^c   Bahk, Jung Dong ^a   Choi, Myung Suk ^a  

^a Gyeongsang National University   (South Korea)

^b BANGALORE UNIVERSITY   (India)

^c UNIVERSITY OF AGRICULTURAL SCIENCES   (India)

Author keywords

Hairy root; Hyoscyamine 6 hydroxylase; Metabolic engineering; Scopolia parviflora; Tropane alkaloids

Indexed keywords

HYOSCYAMINE; HYOSCYAMINE 6BETA HYDROXYLASE; OXYGENASE; SCOPOLAMINE; TROPANE ALKALOID; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRY WEIGHT; EXPRESSION VECTOR; GENE OVEREXPRESSION; GENE SEQUENCE; GENETIC ENGINEERING; GENETIC TRANSFORMATION; NONHUMAN; NORTHERN BLOTTING; PLANT GROWTH; PLANT ROOT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; RHIZOBIUM RHIZOGENES; SCOPOLIA; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; WESTERN BLOTTING;

AGROBACTERIUM RHIZOGENES; SCOPOLIA; SCOPOLIA PARVIFLORA;

EID: 15044353690     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Bonhomme, V., D. Laurain-Matter, and M. A. Fliniaux. 2000. Effects of the rol C gene on hairy root: Induction development and tropane alkaloid production by Atropa belladonna. J. Natural Prod. 63: 1249-1252.
2. Canel, C., M. I. Lopes-Cardoso, S. Whitmer, L. van-der Fits, G. Pasquali, R. van-der Heijden, J. H. C. Hoge, and R. Verpoorte. 1998. Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus. Planta 205: 414-419.
3. Gamberg, O. L., R. A. Miller, and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 148-151.
4. Giri, A. and M. L. Narasu. 2000. Transgenic hairy roots: Recent trends and applications. Biotechnol. Adv. 18: 1-22.
5. Hashimoto, T. and Y. Yamada. 1986. Hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures. Plant Physiol. 81: 619-625.
6. Hashimoto, T., Y. Yukimune, and Y. Yamada. 1986. Tropane alkaloid production in Hyoscyamus roots cultures. Plant Physiol. 124: 61-75.
7. Hamill, J. D., A. J. Parr, M. J. C. Rhodes, R. J. Robins, and N. J. Walton. 1987. New routes to plant secondary products. Biotechnology 5: 800-804.
8. Hilton, M. G. and M. J. C. Rhodes. 1990. Growth and hyoscyamine production of hairy root cultures of Datura stramonium in a modified stirred tank reactor. Appl. Microbiol. Biotechnol. 33: 132-138.
9. Hashimoto, T., D. J. Yun, and Y. Yamada. 1993. Production of tropane alkaloids in genetically engineered root cultures. Phytochemistry 32: 713-718.
10. Jouhikainen, K., L. Lindgren, T. Jokelainen, R. Hiltunen, T. H. Teeri, and K. M. Oksman-Caldentey. 1999. Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208: 545-551.
11. Jeon, Y. H., S. P. Chang, I. Hwang, and Y. H. Kim. 2003. Involvement of gowth-promoting rhizobacterium Paenibacillus polymyxa in root rot of stroded Korean ginseng. J. Microbiol. Biotechnol. 13: 881-891.
12. Katiyar, V. and R. Goel. 2004. Improved plant growth from seed bacterization using siderophore overproducing cold resistant mutant of Pseudomonas fluorescens. J. Microbiol. Biotechnol. 14: 653-657.
13. Kim., B. S., S. I. Jeong, S. W. Cho, J. Nikolovski, D. J. Mooney, S. H. Lee, O. Jeon, T. W. Kim, S. H. Lim, Y. S. Hong, C. Y. Choi, Y. M. Lee, S. H. Kim, and Y. H. Kim. 2003. Tissue engineering of smooth muscle under a mechanically dynamic condition. J. Microbiol. Biotechnol. 13: 881-891.
14. Kang, Y. M., J. Y. Min, H. S. Moon, C. S. Karigar, D. T. Prasad, C. H. Lee, M. S. Choi. 2004. Rapid in vitro adventitious shoot propagation of Scopolia parviflora through rhizome cultures for enhanced production of tropane alkaloids. Plant Cell Reports 23: 128-133.
15. Selvaraj, T., C. Padmanabhan, Y. J. Jeong, and H. Kim. 2004. Occurrence of vesicular-arbuscular mycorrhizal (VAM) fungi and their effect on plant growth in endangered vegetations. J. Microbiol. Biotechnol. 14: 885-890.
16. Matsuda, J., S. Okabe, T. Hashimoto, and Y. Yamada. 1998. Molecular cloning of hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger. J. Biol. Chem. 266: 9460-9464.
17. Tsugawa, H., Y. Otsuki, and Y. Ohashi. 1996. Efficient promoter cassettes for enhanced expression of foreign genes in cotyledonous and monocotyledonous plants. Plant Cell Physiol. 37: 49-59.
18. Rocha, P., O. Stenzel, A. Parr, N. Walton, P. Christon, B. Drager, and M. J. Leech. 2002. Functional expression of tropinone reductase I (tr 1) and hyoscyamine-6β-hydroxylase (h6h) from Hyoscyamus niger in Nicotiana tabacum. Plant Sci. 162: 905-913.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Lab. Press, Cold Spring Harbor, New York, U.S.A.
20. Sato, F., T. Hashimoto, A. Hachiya, K. Tamura, K. B. Choi, T. Morishige, H. Fujimoto, and Y. Yamada. 2001. Metabolic engineering of plant alkaloid biosynthesis. Proc. Natl. Acad. Sci. USA 98: 367-372.
21. Verpoorte, R. and A. W. Alfermann. 2000. Metabolic Engineering of Plant. Secondary Metabolism. Dordrecht, Kluwer Academic Publishers, Netherlands.