Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris

Protein Expression and Purification

Volumn 79, Issue 2, 2011, Pages 217-222

  Jensen, Johanne Mørch ^a   Vester Christensen, Malene Bech ^a   Møller, Marie Sofie ^a   Bønsager, Birgit C ^a   Christensen, Hans Erik Mølager ^a   Hachem, Maher Abou ^a   Svensson, Birte ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Electrospray ionization mass spectrometry; Fed batch bioreactor fermentation; High stability; Limit dextrinase inhibitor; Pullulan

Indexed keywords

ENZYME INHIBITOR; GLUCAN; GLYCOSIDASE; PULLULAN; PULLULANASE; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOREACTOR; CELL COUNT; DRUG ANTAGONISM; DRUG EFFECT; ELECTROSPRAY MASS SPECTROMETRY; ENZYME STABILITY; ENZYMOLOGY; FERMENTATION; GEL CHROMATOGRAPHY; GENETIC TRANSFORMATION; GENETICS; HALF LIFE TIME; HEAT; HORDEUM; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PH; PICHIA; PLANT SEED; PLASMID; SECRETION;

BIOREACTORS; CELL COUNT; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ENZYME INHIBITORS; ENZYME STABILITY; FERMENTATION; GLUCANS; GLYCOSIDE HYDROLASES; HALF-LIFE; HORDEUM; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; PICHIA; PLANT PROTEINS; PLASMIDS; RECOMBINANT PROTEINS; SEEDS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRANSFORMATION, GENETIC;

HORDEUM; PICHIA PASTORIS;

EID: 80051582562     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.04.009     Document Type: Article

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