Molecular analysis, tissue profiles, and seasonal patterns of cytosolic and mitochondrial GPDH in freeze-resistant rainbow smelt (Osmerus mordax)

Physiological and Biochemical Zoology

Volumn 84, Issue 4, 2011, Pages 363-376

  Robinson, Jason L ^a   Hall, Jennifer R ^a   Charman, Mark ^b   Vanya Ewart, K ^b   Driedzic, William R ^a  

^a MEMORIAL UNIVERSITY OF NEWFOUNDLAND   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; GLYCEROL; GLYCEROL 3 PHOSPHATE DEHYDROGENASE; ISOPROTEIN; MESSENGER RNA;

ADAPTATION; ENZYME ACTIVITY; FREEZE TOLERANCE; IMMUNOASSAY; MITOCHONDRIAL DNA; MOLECULAR ANALYSIS; PROTEIN; SALMONID; SEASONAL VARIATION;

ACCLIMATIZATION; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BLOOD; CANADA; COMPARATIVE STUDY; CYTOSOL; ELECTROPHORESIS; ENZYMOLOGY; FREEZING; GENE DOSAGE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; LIVER; METABOLISM; MITOCHONDRION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OSMERIFORMES; PHYLOGENY; POLYMERASE CHAIN REACTION; SALMON; SEASON; SPECIES DIFFERENCE;

ACCLIMATIZATION; AMINO ACID SEQUENCE; ANIMALS; CYTOSOL; DNA, COMPLEMENTARY; ELECTROPHORESIS; FREEZING; GENE DOSAGE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLYCEROL; GLYCEROLPHOSPHATE DEHYDROGENASE; LIVER; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; NEWFOUNDLAND AND LABRADOR; OSMERIFORMES; PHYLOGENY; POLYMERASE CHAIN REACTION; PROTEIN ISOFORMS; RNA, MESSENGER; SALMON; SEASONS; SPECIES SPECIFICITY;

ANURA; DANIO RERIO; MALLOTUS (FISH); OSMERUS; OSMERUS MORDAX; SALMO SALAR; TELEOSTEI;

EID: 80051483758     PISSN: 15222152     EISSN: None     Source Type: Journal    
DOI: 10.1086/660162     Document Type: Article

References (42)

1. Ballantyne J.S. 1994. Fish mitochondria. Pp. 487-501 in P.W. Hochachka and T.P. Mommsen, eds. Biochemistry and Molecular Biology of Fishes. Vol. 3. Techniques. Elsevier Science, Amsterdam.
2. Basaglia F. and C. Cucchi. 1993. Effects of benzidine on the electrophoretic behavior of some enzymes in Ictalurus species (Ictaluridae, Teleostei). Comp Biochem Physiol C 104:225-232.
3. Basaglia F. 1995. Variations induced by phenylhydrazine on glycerol-3-phosphate dehydrogenase isozymes in black bullhead, Ictalurus melas (Silurifmes, Ictaluridae). Cytobios 82:139-150.
4. Brown L.J., M.J. MacDonald, D.A. Lehn, and S.M. Moran. 1994. Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA: evidence for EF-hand calcium-binding domains. J Biol Chem 269:14363-14366.
5. Clow K.A., K.V. Ewart, and W.R. Driedzic. 2008. Low temperature directly activates the initial glycerol antifreeze response in isolated rainbow smelt (Osmerus mordax) liver cells. Am J Physiol 295:R961-R970.
6. Ditlecadet D., C.E. Short, and W.R. Driedzic. 2011. Glycerol loss to water exceeds glycerol catabolism via glycerol kinase in freeze-resistant rainbow smelt (Osmerus mordax). Am J Physiol 300:R674-R684.
7. Driedzic W.R., K.A. Clow, C.E. Short, and K.V. Ewart. 2006. Glycerol production in the rainbow smelt (Osmerus mordax) may be triggered by low temperature alone and is associated with the activation of the glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase. J Exp Biol 209:1016-1023.
8. Driedzic W.R. and K.V. Ewart. 2004. Control of glycerol production by rainbow smelt (Osmerus mordax) to provide freeze resistance and allow foraging at low winter temperatures. Comp Biochem Physiol B 139:347-357.
9. Driedzic W.R. and C.E. Short. 2007. Relationship between food availability, glycerol, and glycogen levels in low-temperature challenged rainbow smelt Osmerus mordax. J Exp Biol 210: 2866-2872.
10. Driedzic W.R., J.L. West, D.H. Sephton, and J.A. Raymond. 1998. Enzyme activity levels associated with the production of glycerol as an antifreeze in liver of rainbow smelt (Osmerus mordax). Fish Physiol Biochem 18:125-134.
11. Ewart K.V., R.C. Richards, and W.R. Driedzic. 2001. Cloning of glycerol-3-phosphate dehydrogenase cDNA from two fish species and effect of temperature on enzyme expression in rainbow smelt (Osmerus mordax). Comp Biochem Physiol B 281:401-412.
12. Fletcher G.L., M.H. Kao, and J.B. Dempson. 1988. Lethal freezing temperatures of arctic char and other salmonids in the presence of ice. Aquaculture 71:369-378.
13. Gardner R.S. 1974. A sensitive colorimetric assay for mitochondrial alpha-glycerophosphate dehydrogenase. Anal Biochem 59:272-276.
14. Joanisse D.R. and K.B. Storey. 1994. Enzyme activity profiles in an overwintering population of freeze-tolerant larvai of the gall fly, Eurosta solidaginis. J Comp Physiol B 164:247-255.
15. Klingenberg M. 1970. Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247-252.
16. Koza R.A., U.C. Kozak, L.J. Brown, E.H. Leiter, M.J. MacDonald, and L.P. Kozak. 1996. Sequence- and tissue-dependent RNA expression of mouse FAD-linked glycerol-3-phosphate dehydrogenase. Arch Biochem Biophys 336:97-104.
17. Lewis J.M., K.V. Ewart, and W.R. Driedzic. 2004. Freeze resistance in rainbow smelt (Osmerus mordax): seasonal pattern of glycerol and antifreeze protein levels and liver enzyme activity associated with glycerol production. Physiol Biochem Zool 77:415-422.
18. Liebscher R.S., R.C. Richards, J.M. Lewis, C.E. Short, D.M. Muise, W.R. Driedzic, and K.V. Ewart. 2006. Seasonal freeze resistance of rainbow smelt (Osmerus mordax) is generated by differential expression of glycerol-3-phosphate dehydrogenase, phosphoenolpyruvate carboxykinase, and antifreeze protein genes. Physiol Biochem Zool 79:411-423.
19. -ΔΔCT method. Methods 25:402-408.
20. Magnoni L., E. Vaillancourt, and J.M. Weber. 2008. In vivo regulation of rainbow trout lipolysis by catecholamines. J Exp Biol 211:2460-2466.
21. Matsutani A., Y. Takeuchi, H. Ishihara, S. Kuwano, and Y. Oka. 1996. Molecular cloning of human mitochondrial glycerophosphate dehydrogenase gene: genomic structure, chromosomal localization, and existence of a pseudogene. Biochem Biophys Res Commun 223:481-486.
22. Müller S. and H.J. Seitz. 1994. Cloning of a cDNA for the FADlinked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones. Proc Natl Acad Sci USA 91:10581-10585.
23. Nolan T., R.E. Hands, and S.A. Bustin. 2006. Quantification of mRNA using real-time RT-PCR. Nat Protoc 1:1559-1582.
24. Pfaffl M.W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29:e45.
25. Phillip D.P., W.F. Childers, and G.S. Whitt. 1979. Evolution of patterns of differential gene expression: a comparison of the temporal and spatial patterns of isozyme locus expression in two closely related fish species northern largemouth bass, Micropterus salmoides salmoides, and smallmouth bass, Micropterus dolomieui. J Exp Zool 210:473-488.
26. Piquet M.A., E. Fontaine, B. Sibille, C. Filippi, C. Keriel, and X.M. Leverve. 1996. Uncoupling effect of polyunsaturated fatty acid deficiency in isolated rat hepatocytes: effect on glycerol metabolism. Biochem J 317:667-674.
27. Rauchova H., G. Zacharova, and T. Soukup. 2004. Influence of chronically altered thyroid status on the activity of liver mitochondrial glycerol-3-phosphate dehydrogenase in female inbred Lewis rats. Horm Metab Res 36:286-290.
28. Raymond J.A. 1992. Glycerol is a colligative antifreeze in some northern fishes. J Exp Zool 262:347-352.
29. Raymond J.A. 1993. Glycerol and water balance in a near-ososmotic teleost, winter-acclimatized rainbow smelt. Can J Zool 71: 1849-1854.
30. Raymond J.A. 1995. Glycerol synthesis in the rainbow smelt Osmerus mordax. J Exp Biol 198:2569-2573.
31. Raymond J.A. and W.R. Driedzic. 1997. Amino acids are a source of glycerol in cold-acclimatized rainbow smelt. Comp Biochem Physiol B 118:387-393.
32. Raymond J.A. and A. Hassel. 2000. Some characteristics of freezing avoidance in two osmerids, rainbow smelt and capelin. J Fish Biol 57:1-7.
33. Rothe G.M. 1994. Electrophoresis of Enzymes: Laboratory Methods. Springer, Berlin.
34. Saitou N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425.
35. Smith C.D. and J.G. Eales. 1971. Influence of acclimation temperature on rate constants for thyroid uptake of iodide by brook trout, Salvelinus fontinalis (Mitchill). Can J Zool 49: 783-786.
36. Taleux N., B. Guigas, H. Dubouchaud, M. Moreno, J.M. Weitzel, F. Goglia, R. Favier, and X.M. Leverve. 2009. High expression of thyroid hormone receptors and mitochondrial glycerol-3-phosphate dehydrogenase in the liver is linked to enhanced fatty acid oxidation in Lou/C, a rat strain resistant to obesity. J Biol Chem 284:4308-4316.
37. Tamura K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599.
38. Thompson J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.
39. Treberg J.R., J.M. Lewis, and W.R. Driedzic. 2002a. Comparison of liver enzymes in osmerid fishes: key differences between a glycerol accumulating species, rainbow smelt (Osmerus mordax) and a species that does not accumulate glycerol, capelin (Mallotus villosus). Comp Biochem Physiol A 132: 433-438.
40. Treberg J.R., C.E. Wilson, R.C. Richards, K.V. Ewart, and W.R. Driedzic. 2002b. The freeze-avoidance response of smelt Osmerus mordax: initiation and subsequent suppression of glycerol, trimethylamine oxide and urea accumulation. J Exp Biol 205:1419-1427.
41. Van der Bank F.H., W.S. Grant, and J.T. Ferreira. 1989. Electrophoretically detectable genetic data for fifteen southern African cichlids. J Fish Biol 34:465-483.
42. 13C nuclear magnetic resonance study. J Exp Zool 305A:480-488.