The use of multiple typing methods allows a more accurate molecular characterization of Vibrio parahaemolyticus strains isolated from the Italian Adriatic Sea

FEMS Microbiology Ecology

Volumn 77, Issue 3, 2011, Pages 611-622

  Caburlotto, Greta ^a   Lleo, Maria M ^a   Gennari, Micol ^a   Balboa, Sabela ^b   Romalde, Jesus L ^b  

^a UNIVERSITY OF VERONA   (Italy)

^b UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Bacterial clones' persistence in the marine environment; Marine Vibrio parahaemolyticus; Molecular typing of environmental bacterial strains; Vibrio molecular typing

Indexed keywords

BACTERIUM; CLONE; ELECTROKINESIS; EPIDEMIOLOGY; GASTROENTERITIS; GENETIC VARIATION; HEALTH IMPACT; INFECTIOUS DISEASE; INGESTION RATE; MARINE ENVIRONMENT; MICROBIAL COMMUNITY; MOLECULAR ANALYSIS; PLANKTON; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; SEAFOOD; TEMPORAL DISTRIBUTION; WOUNDING;

ARTICLE; BACTERIUM IDENTIFICATION; CLASSIFICATION; DNA FINGERPRINTING; EVALUATION; GASTROENTERITIS; GENETICS; HUMAN; ISOLATION AND PURIFICATION; ITALY; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PLANKTON; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SEA FOOD; SEDIMENT; VIBRIO PARAHAEMOLYTICUS; VIBRIOSIS;

BACTERIAL TYPING TECHNIQUES; DNA FINGERPRINTING; ELECTROPHORESIS, GEL, PULSED-FIELD; GASTROENTERITIS; GEOLOGIC SEDIMENTS; HUMANS; ITALY; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANKTON; POLYMERASE CHAIN REACTION; SEAFOOD; VIBRIO INFECTIONS; VIBRIO PARAHAEMOLYTICUS;

ADRIATIC SEA; ITALY; MEDITERRANEAN SEA;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; VIBRIO; VIBRIO PARAHAEMOLYTICUS;

EID: 79961173501     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2011.01142.x     Document Type: Article

References (37)

1. Bhanumathi R, Sabeena F, Isac SR, Shukla BN & Singh DV (2003) Molecular characterization of Vibrio cholerae O139 Bengal isolated from water and the aquatic plant Eichhornia crassipes in the River Ganga, Varanasi, India. Appl Environ Microb 69: 2389-2394.
2. Bourassa L & Camilli A (2009) Glycogen contributes to the environmental persistence and transmission of Vibrio cholerae. Mol Microbiol 72: 124-138.
3. Broza M, Gancz H & Kashi Y (2008) The association between non-biting midges and Vibrio cholerae. Environ Microbiol 10: 3193-3200.
4. Caburlotto G, Ghidini V, Gennari M, Tafi MC & Lleo MM (2008) Isolation of a Vibrio parahaemolyticus pandemic strain from a marine water sample obtained in the northern Adriatic. Euro Surveill 13.
5. Caburlotto G, Gennari M, Ghidini V, Tafi MC & Lleo MM (2009) Presence of virulence-related genes in tdh-negative Vibrio parahaemolyticus environmental strains isolated from marine samples in the area of the Venetian Lagoon, Italy. FEMS Microbiol Ecol 70: 506-514.
6. Caburlotto G, Gennari M, Ghidini V, Tafi M & Lleo MM (2010a) Serological and molecular characterization of Vibrio parahaemolyticus marine strains carrying pandemic genetic markers. ISME J 4: 1071-1074.
7. Caburlotto G, Lleo MM, Hilton T, Huq A, Colwell RR & Kaper JB (2010b) Effect on human cells of environmental Vibrio parahaemolyticus strains carrying type III secretion system 2. Infect Immun 78: 3280-3287.
8. Chakraborty S, Nair GB & Shinoda S (1997) Pathogenic vibrios in the natural aquatic environment. Rev Environ Health 12: 63-80.
9. Colombo MM, Mastrandrea S, Leite F, Santona A, Uzzau S, Rappelli P, Pisano M, Rubino S & Cappuccinelli P (1997) Tracking of clinical and environmental Vibrio cholerae O1 strains by combined analysis of the presence of toxin cassette, plasmid content and ERIC PCR. FEMS Immunol Med Mic 19: 33-45.
10. Colwell RR (1996) Global climate and infectious disease: the cholera paradigm. Science 274: 2025-2031.
11. De Paola A, Ulaszek J, Kaysner CA, Tenge BJ, Nordstrom JL, Well J, Puhr N & Gendel SM (2003) Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in North America and Asia. Appl Environ Microb 69: 3999-4005.
12. Dice R (1945) Measures of the amount of ecological association between species. Ecology 26: 297-302.
13. Eiler A, Gonzalez-Rey C, Allen S & Bertilsson S (2007) Growth response of Vibrio cholerae and other Vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water. FEMS Microbiol Ecol 60: 411-418.
14. Huq A, West PA, Small EB, Huq MI & Colwell RR (1984) Influence of water temperature, salinity and pH on survival and growth of toxigenic Vibrio cholerae serovar O1 associated with live copepods in laboratory microcosms. Appl Environ Microb 48: 420-424.
15. Hulton CSJ, Higgins CF & Sharp PM (1991) ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium, and other enterobacteria. Mol Microbiol 5: 825-834.
16. Kam KM, Luey CK, Vibrio parahaemolyticus PulseNet PFGE Protocol Working Group et al. (2008) Evaluation and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping Vibrio parahaemolyticus: an international multicenter collaborative study. J Clin Microbiol 46: 2766-2773.
17. Kingston JJ, Zachariah K, Tuteja U, Kumar S & Batra HV (2009) Molecular characterization of Vibrio cholerae isolates from cholera outbreaks in North India. J Microbiol 47: 110-115.
18. Labella A, Manchado M, Alonso MC, Castro D, Romalde JL & Borrego JJ (2010) Molecular intraspecific characterization of Photobacterium damselae ssp. damselae strains affecting cultured marine fish. J Appl Microbiol 108: 2122-2132.
19. Lhafi SK & Kuhne M (2007) Occurrence of Vibrio spp. in blue mussels (Mytilus edulis) from the German Wadden Sea. Int J Food Microbiol 116: 297-300.
20. Lipp EK, Huq A & Colwell RR (2002) Effects of global climate on infectious disease: the cholera model. Clin Microbiol Rev 15: 757-770.
21. Lizárraga-Partida ML & Quilici ML (2009) Molecular analyses of Vibrio cholerae O1 clinical strains, including new nontoxigenic variants isolated in Mexico during the Cholera epidemic years between 1991 and 2000. J Clin Microbiol 47: 1364-1371.
22. Maluping RP, Ravelo C, Lavilla-Pitogo CR, Krovacek K & Romalde JL (2005) Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR-based methods. J Appl Microbiol 99: 383-391.
23. Olive DM & Bean P (1999) Principles and applications of methods for DNA-based typing of microbial organisms. J Clin Microbiol 37: 1661-1669.
24. Pruzzo C, Vezzulli L & Colwell RR (2008) Global impact of Vibrio cholerae interactions with chitin. Environ Microbiol 10: 1400-1410.
25. Rao BM & Surendran PK (2010) Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches. Lett Appl Microbiol 51: 65-74.
26. Rawlings TK, Ruiz GM & Colwell RR (2007) Association of Vibrio cholerae O1 El Tor and O139 Bengal with the Copepods Acartia tonsa and Eurytemora affinis. Appl Environ Microb 73: 7926-7933.
27. Rivera IG, Chowdhury MA, Huq A, Jacobs D, Martins MT & Colwell RR (1995) Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains. Appl Environ Microb 61: 2898-2904.
28. Rodríguez JM, López-Romalde S, Beaz R, Alonso MC, Castro D & Romalde JL (2006) Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD. Dis Aquat Organ 69: 175-183.
29. Silva-Rubio A, Acevedo C, Magariños B, Jaureguiberry B, Toranzo AE & Avendaño-Herrera R (2008) Antigenic and molecular characterization of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chile. Dis Aquat Organ 79: 27-35.
30. Sneath PHA & Sokal RR (1973) Numerical Taxonomy. The Principles and Practice of Numerical Classification. W.H. Freeman, San Francisco, CA.
31. Stern MJ, Ferro-Luzzi Ames G, Smith NH, Robinson EC & Higgins F (1984) Repetitive extragenic palindromic sequences: a major component of the bacterial genome. Cell 37: 1015-1026.
32. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH & Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulse-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33: 2233-2239.
33. Van Belkum A, Struelens M, de Visser A, Verbrugh H & Tibayrenc M (2001) Role of genomic typing in taxonomy, evolutionary genetics, and microbial epidemiology. Clin Microbiol Rev 14: 547-560.
34. Versalovic J, Koeuth T & Lupsky JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19: 6823-6831.
35. Versalovic J, Woods CR, Koeuth T & Lupsky JR (1993) Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. J Clin Microbiol 31: 1927-1931.
36. Vezzulli L, Pezzati E, Moreno M, Fabiano M, Pane L & Pruzzo C and the VibrioSea Consortium (2009) Benthic ecology of Vibrio spp. and pathogenic Vibrio species in a coastal Mediterranean environment (La Spezia Gulf, Italy). Microb Ecol 58: 808-818.
37. Wong H & Lin C (2001) Evaluation of typing of Vibrio parahaemolyticus by three PCR methods using specific primers. J Clin Microbiol 39: 4233-4240.