Antihypertensive activity of transgenic rice seed containing an 18-repeat novokinin peptide localized in the nucleolus of endosperm cells

Plant Biotechnology Journal

Volumn 9, Issue 7, 2011, Pages 729-735

  Wakasa, Yuhya ^a   Zhao, Hui ^b,c   Hirose, Sakiko ^a,d   Yamauchi, Daiki ^b   Yamada, Yuko ^b   Yang, Lijun ^a   Ohinata, Kousaku ^b   Yoshikawa, Masaaki ^b,c   Takaiwa, Fumio ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c RESEARCH INSTITUTE FOR PRODUCTION DEVELOPMENT   (Japan)

^d NATIONAL INSTITUTE OF CROP SCIENCE   (Japan)

Author keywords

Bioactive peptide; Hypertension; Oryza sativa L.; Transgenic rice

Indexed keywords

ANTIHYPERTENSIVE AGENT; ARG PRO LEU LYS PRO TRP; ARG-PRO-LEU-LYS-PRO-TRP; GLUTEN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; OLIGOPEPTIDE; PANCREATIN; PEPSIN A; PEPTIDE FRAGMENT;

ANIMAL; ARTICLE; BIOSYNTHESIS; BLOOD PRESSURE; CHEMISTRY; DRUG EFFECT; ENDOSPERM; GENETICS; HYPERTENSION; MALE; METABOLISM; NUCLEOLUS; ORAL DRUG ADMINISTRATION; PLANT SEED; RAT; RICE; SPONTANEOUSLY HYPERTENSIVE RAT; TANDEM REPEAT; TIME; TRANSGENIC PLANT; ULTRASTRUCTURE;

ADMINISTRATION, ORAL; ANIMALS; ANTIHYPERTENSIVE AGENTS; BLOOD PRESSURE; CELL NUCLEOLUS; ENDOSPERM; GLUTENS; GREEN FLUORESCENT PROTEINS; HYPERTENSION; MALE; OLIGOPEPTIDES; ORYZA SATIVA; PANCREATIN; PEPSIN A; PEPTIDE FRAGMENTS; PLANTS, GENETICALLY MODIFIED; RATS; RATS, INBRED SHR; RECOMBINANT FUSION PROTEINS; SEEDS; TANDEM REPEAT SEQUENCES; TIME FACTORS;

ALLIUM CEPA; ORYZA SATIVA; RATTUS;

EID: 79961154061     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2010.00576.x     Document Type: Article

References (14)

1. st century: where is the gold? J. Hypertens. 22, 2243-2251.
2. Goto, F., Yoshihara, T., Shigemoto, N., Toki, S. and Takaiwa, F. (1999) Iron fortification of rice seed by the soybean ferritin gene. Nat. Biotechnol. 17, 282-286.
3. Kearney, P.M., Whelton, M., Reynolds, K., Whelton, P.K. and He, J. (2004) Worldwide prevalence of hypertensions: a systematic review. J. Hypertens. 22, 21-24.
4. Matoba, N., Yamada, Y., Usui, H., Nakagiri, R. and Yoshikawa, M. (2001) Designing potent derivatives of ovokinin (2-7), an anti-hypertensive peptide derived from ovalbumin. Biosci. Biotechnol. Biochem. 65, 736-739.
5. Murray, M.G. and Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8, 4321-4325.
6. Nicolson, D.J., Dickinson, H.O., Campbell, F. and Mason, J.M. (2004) Lifestyle interventions or drugs for patients with essential hypertension: a systematic review. J. Hypertens. 22, 2243-2251.
7. Onishi, K., Matoba, N., Yamada, Y., Doyama, N., Utsumi, S. and Yoshikawa, M. (2004) Optimal designing of β-conglycinin to genetically incorporate RPLKPW, a potent anti-hypertensive peptide. Peptides 25, 37-43.
8. Oono, Y., Wakasa, Y., Hirose, S., Yang, L., Sakuta, C. and Takaiwa, F. (2010) Analysis of ER stress in developing rice endosperm accumulating β-amyloid peptide. Plant Biotechnol. J. 8, 1-28.
9. Takaiwa, F., Kikuchi, S. and Oono, K. (1987) Major type of glutelin mRNAs can be divided into two classes. Mol. Gen. Genet. 208, 15-22.
10. Wakasa, Y., Yasuda, H. and Takaiwa, F. (2006) High accumulation of bioactive peptide in transgenic rice seeds by expression of introduced multiple genes. Plant Biotechnol. J. 4, 499-510.
11. Wakasa, Y., Yang, L., Hirose, S. and Takaiwa, F. (2009) Expression of unprocessed glutelin precursor alters polymerization without affecting trafficking and accumulation. J. Exp. Bot. 12, 3503-3511.
12. 3 receptor in mice. Peptides, 30, 735-739.
13. Yang, L., Tada, Y., Yamamoto, P.M., Zhao, H., Yoshikawa, M. and Takaiwa, F. (2006) A transgenic-rice seed accumulating an anti-hypertensive peptide reduces the blood pressure of spontaneously hypertensive rats. FEBS Lett. 580, 3315-3320.
14. Yasuda, Y., Hayashi, Y., Jomori, T. and Takaiwa, F. (2006) The correlation between expression and localization of a foreign gene product in rice endosperm. Plant Cell Physiol. 47, 756-763.