Transwell® Invasion Assays

Methods in Molecular Biology

Volumn 769, Issue , 2011, Pages 97-110

  Marshall, John ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Fluoroblok; Invasion; Matrigel; Transwell

Indexed keywords

COLLAGEN; COLLAGEN TYPE 1; LAMININ; MATRIGEL; PROTEOGLYCAN;

ARTICLE; CANCER INVASION; CELL MIGRATION; CHEMISTRY; CULTURE TECHNIQUE; DRUG COMBINATION; GEL; HUMAN; INSTRUMENTATION; METHODOLOGY; STAINING; TUMOR CELL LINE; ULTRASTRUCTURE;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL MIGRATION ASSAYS; COLLAGEN; COLLAGEN TYPE I; DRUG COMBINATIONS; GELS; HUMANS; LAMININ; NEOPLASM INVASIVENESS; PROTEOGLYCANS; STAINING AND LABELING;

EID: 79960918651     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-207-6_8     Document Type: Chapter

References (19)

1. Dorudi S and Hart IR (1993) Mechanisms underlying invasion and metastasis Curr Opin Oncol 5:130–135
2. Hanahan D and Weinberg RA (2000) The Hallmarks of Cancer. J Cell 100:57–70
3. Gupta GP and Massague J (2006) Cancer Metastasis: Building a framework. Cell 127: 679–695
4. Orimo A and Weinberg RA (2006) Stromal fibroblasts in cancer: a novel tumour-promot-ing cell type cell Cycle 5:1597–1601
5. Petras K and Ostman A (2010) Hallmarks of cancer: interactions with the tumour stroma Exp Cell Res 316:1324–1331
6. Mantovani A, Allavena P, Sica A and Balkwill F (2008) Cancer-related inflammation Nature 454:436–444
7. Colotta F, Allavena P, Sica A, Garlanda C and Mantovani A. (2009) Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability. Carcinogenesis 30:1073–1081
8. Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes J Exp Med 115: 453–466
9. Kleinmann HK and Martin GR (2005) Matrigel: Basement membrane matrix with biological activity Seminars Cancer Biol. 15:378–386
10. Kalluri R (2003) Basement membranes: structure, assembly and role in tumour angiogenesis Nat Rev Cancer 3:422–433
11. Timpl R, Rohde H, Robey PG, Rennard SI, Foidart JM, Martin GR (1979) Laminin-a glycoprotein from basement membranes J Biol Chem 254:9933–9937
12. Virtanen I, Gullberg D, Rissanen J, Kivilaakso E, Kiviluoto T, Laitinen LA, Lehto V-P and Ekblom P (2000) Laminin a1-chain shows a restricted distribution in epithelial basement membranes of fetal and adult tissues Exp Cell res 257:298–309
13. Sodek, KL, Brown TJ and Ringuette MJ. (2008) Collagen I but not Matrigel matrices provide MMP-dependent barrier to ovarian cancer cell penetration. BMC Cancer 8:223
14. Reiser K, McCormick RJ, Rucker RB (1992) Enzymatic and n on-enzymatic cross-linking of collagen and elastin FASEB J 6:2439–2449
15. Sabeh F, Shimizu-Hirota R and Weiss SJ (2010) Protease-dependent versus independent cancer cell invasion programs: three-dimensional amoeboid movement revisited. J Cell Biol 185:11–19
16. Ota I, Li X-Y, Hu Y and Weiss SJ (2009) Induction of a MT1-MMP and MT2-MMP-dependent basement membrane transmigration program in cancer cells by Snail1 PNAS 106:20318–20323
17. Thomas GJ, Poomswat S, Lewis MP, Hart IR, Speight PM and Marshall JF (2001) avb6 integrin upregulates matrix metalloproteinase 9 and promotes migration of normal oral kera-tinocytes J Invest Dermatol 116:898–904
18. Hooper S, Marshall JF, Sahai E. (2006) Tumor cell migration in three dimensions. Methods Enzymol.;406:625–43
19. Lamb RF, Hennigan RF, Turnbull K, Katsankis KD, MacKenzie ED, Birnie GD and Ozanne BW (1997) AP-1 mediated invasion requires increased expression of the hyaluronan receptor CD44 Mol Cell Biol 17:963–976