Establishment of an immortalized human endometrial stromal cell line with functional responses to ovarian stimuli

Reproductive Biology and Endocrinology

Volumn 9, Issue , 2011, Pages

  Yuhki, Munehiro ^a   Kajitani, Takashi ^b   Mizuno, Takakazu ^a   Aoki, Yuko ^a   Maruyama, Tetsuo ^b  

^a CHUGAI PHARMACEUTICAL CO LTD   (Japan)

^b KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; BUCLADESINE; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN; CYCLIC AMP; CYCLOOXYGENASE 2; CYTOKERATIN; ESTRADIOL; ESTROGEN RECEPTOR; INTERLEUKIN 1BETA; MEDROXYPROGESTERONE ACETATE; MICROSOMAL AMINOPEPTIDASE; PROGESTERONE RECEPTOR A; PROGESTERONE RECEPTOR B; PROSTAGLANDIN E2; SEX HORMONE; SOMATOMEDIN BINDING PROTEIN 1; TELOMERASE REVERSE TRANSCRIPTASE; VIMENTIN; MEMBRANE METALLOENDOPEPTIDASE; PROGESTERONE RECEPTOR;

ARTICLE; CELL IMMORTALIZATION; CELL LINE; CELL STIMULATION; CELL STRAIN KC02 44D; CONTROLLED STUDY; CYTOSKELETON; DECIDUALIZATION; ENDOMETRIOSIS; ENDOMETRIUM; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; MARKER GENE; MEDICAL RESEARCH; OVARY; PHENOTYPE; STROMA; BIOSYNTHESIS; CYTOLOGY; DECIDUA; DOWN REGULATION; DRUG EFFECT; METABOLISM; MIDDLE AGED; PHYSIOLOGY; STROMA CELL;

ANTIGENS, CD13; BUCLADESINE; CELL LINE; DECIDUA; DOWN-REGULATION; ENDOMETRIUM; ESTRADIOL; FEMALE; HUMANS; INTERLEUKIN-1BETA; MEDROXYPROGESTERONE ACETATE; MIDDLE AGED; NEPRILYSIN; RECEPTORS, ESTROGEN; RECEPTORS, PROGESTERONE; STROMAL CELLS; VIMENTIN;

EID: 79960853676     PISSN: None     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/1477-7827-9-104     Document Type: Article

References (32)

1. Ing NH, Spencer TE, Bazer FW. Estrogen enhances endometrial estrogen receptor gene expression by a posttranscriptional mechanism in the ovariectomized ewe. Biol Reprod 1996, 54:591-599. 10.1095/biolreprod54.3.591, 8835380.
2. Kurita T, Lee KJ, Cooke PS, Lydon JP, Cunha GR. Paracrine regulation of epithelial progesterone receptor and lactoferrin by progesterone in the mouse uterus. Biol Reprod 2000, 62:831-838. 10.1095/biolreprod62.4.831, 10727250.
3. Nephew KP, Long X, Osborne E, Burke KA, Ahluwalia A, Bigsby RM. Effect of estradiol on estrogen receptor expression in rat uterine cell types. Biol Reprod 2000, 62:168-177. 10.1095/biolreprod62.1.168, 10611082.
4. Gellersen B, Brosens J. Cyclic AMP and progesterone receptor cross-talk in human endometrium: a decidualizing affair. J Endocrinol 2003, 178:357-372. 10.1677/joe.0.1780357, 12967329.
5. Bergqvist A, Bruse C, Carlberg M, Carlström K. Interleukin 1β, interleukin-6, and tumor necrosis factor-α in endometriotic tissue and in endometrium. Fertil Steril 2001, 75:489-495. 10.1016/S0015-0282(00)01752-0, 11239529.
6. Hudelist G, Lass H, Keckstein J, Walter I, Wieser F, Wenzl R, Mueller R, Czerwenka K, Kubista E, Singer CF. Interleukin 1α and tissue-lytic matrix metalloproteinase-1 are elevated in ectopic endometrium of patients with endometriosis. Hum Reprod 2005, 20:1695-1701. 10.1093/humrep/deh794, 15746198.
7. Chapdelaine P, Kang J, Boucher-Kovalik S, Caron N, Tremblay JP, Fortier MA. Decidualization and maintenance of a functional prostaglandin system in human endometrial cell lines following transformation with SV40 large T antigen. Mol Hum Reprod 2006, 12:309-319. 10.1093/molehr/gal034, 16556676.
8. Barbier CS, Becker KA, Troester MA, Kaufman DG. Expression of exogenous human telomerase in cultures of endometrial stromal cells does not alter their hormone responsiveness. Biol Reprod 2005, 73:106-114. 10.1095/biolreprod.104.035063, 15772261.
9. Krikun G, Mor G, Alvero A, Guller S, Schatz F, Sapi E, Rahman M, Caze R, Qumsiyeh M, Lockwood CJ. A novel immortalized human endometrial stromal cell line with normal progestational response. Endocrinology 2004, 145:2291-2296. 10.1210/en.2003-1606, 14726435.
10. Samalecos A, Reimann K, Wittmann S, Schulte HM, Brosens JJ, Bamberger AM, Gellersen B. Characterization of a novel telomerase-immortalized human endometrial stromal cell line, St-T1b. Reprod Biol Endocrinol 2009, 7:76. 10.1186/1477-7827-7-76, 2719639, 19619280.
11. Tamura K, Yoshie M, Hara T, Isaka K, Kogo H. Involvement of stathmin in proliferation and differentiation of immortalized human endometrial stromal cells. J Reprod Dev 2007, 53:525-533. 10.1262/jrd.18129, 17272923.
12. Nagashima T, Maruyama T, Uchida H, Kajitani T, Arase T, Ono M, Oda H, Kagami M, Masuda H, Nishikawa S, Asada H, Yoshimura Y. Activation of SRC kinase and phosphorylation of signal transducer and activator of transcription-5 are required for decidual transformation of human endometrial stromal cells. Endocrinology 2008, 149:1227-1234.
13. Brosens JJ, Hayashi N, White JO. Progesterone receptor regulates decidual prolactin expression in differentiating human endometrial stromal cells. Endocrinology 1999, 140:4809-4820. 10.1210/en.140.10.4809, 10499541.
14. Borthwick JM, Charnock-Jones DS, Tom BD, Hull ML, Teirney R, Phillips SC, Smith SK. Determination of the transcript profile of human endometrium. Mol Hum Reprod 2003, 9:19-33. 10.1093/molehr/gag004, 12529417.
15. Brandenberger AW, Lebovic DI, Tee MK, Ryan IP, Tseng JF, Jaffe RB, Taylor RN. Oestrogen receptor (ER)-α and ER-β isoforms in normal endometrial and endometriosis-derived stromal cells. Mol Hum Reprod 1999, 5:651-655. 10.1093/molehr/5.7.651, 10381820.
16. Classen-Linke I, Kusche M, Knauthe R, Beier HM. Establishment of a human endometrial cell culture system and characterization of its polarized hormone responsive epithelial cells. Cell Tissue Res 1997, 287:171-185.
17. Milgrom E, Thi L, Atger M, Baulieu EE. Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus. J Biol Chem 1973, 248:6366-6374.
18. Saegusa M, Okayasu I. Changes in expression of estrogen receptors α and β in relation to progesterone receptor and pS2 status in normal and malignant endometrium. Jpn J Cancer Res 2000, 91:510-518.
19. Shiozawa T, Li SF, Nakayama K, Nikaido T, Fujii S. Relationship between the expression of cyclins/cyclin-dependent kinases and sex-steroid receptors/Ki67 in normal human endometrial glands and stroma during the menstrual cycle. Mol Hum Reprod 1996, 2:745-752. 10.1093/molehr/2.10.745, 9239692.
20. Cheong YC, Shelton JB, Laird SM, Richmond M, Kudesia G, Li TC, Ledger WL. IL-1, IL-6 and TNF-α concentrations in the peritoneal fluid of women with pelvic adhesions. Hum Reprod 2002, 17:69-75.
21. Keenan JA, Chen TT, Chadwell NL, Torry DS, Caudle MR. IL-1β, TNF-α, and IL-2 in peritoneal fluid and macrophage-conditioned media of women with endometriosis. Am J Reprod Immunol 1995, 34:381-385.
22. Mori H, Sawairi M, Nakagawa M, Itoh N, Wada K, Tamaya T. Peritoneal fluid interleukin-1β and tumor necrosis factor in patients with benign gynecologic disease. Am J Reprod Immunol 1991, 26:62-67.
23. Klemmt PA, Carver JG, Kennedy SH, Koninckx PR, Mardon HJ. Stromal cells from endometriotic lesions and endometrium from women with endometriosis have reduced decidualization capacity. Fertil Steril 2006, 85:564-572. 10.1016/j.fertnstert.2005.08.046, 1626574, 16500320.
24. Frank GR, Brar AK, Jikihara H, Cedars MI, Handwerger S. Interleukin-1β and the endometrium: an inhibitor of stromal cell differentiation and possible autoregulator of decidualization in humans. Biol Reprod 1995, 52:184-191. 10.1095/biolreprod52.1.184, 7536045.
25. Kariya M, Kanzaki H, Takakura K, Imai K, Okamoto N, Emi N, Kariya Y, Mori T. Interleukin-1 inhibits in vitro decidualization of human endometrial stromal cells. J Clin Endocrinol Metab 1991, 73:1170-1174. 10.1210/jcem-73-6-1170, 1955497.
26. Mizuno K, Tanaka T, Umesaki N, Ogita S. Inhibition of cAMP-mediated decidualization in human endometrial stromal cells by IL-1β and laminin. Horm Metab Res 1999, 31:307-310. 10.1055/s-2007-978742, 10422725.
27. Yoshino O, Osuga Y, Hirota Y, Koga K, Hirata T, Yano T, Ayabe T, Tsutsumi O, Taketani Y. Endometrial stromal cells undergoing decidualization down-regulate their properties to produce proinflammatory cytokines in response to interleukin-1β via reduced p38 mitogen-activated protein kinase phosphorylation. J Clin Endocrinol Metab 2003, 88:2236-2241. 10.1210/jc.2002-021788, 12727980.
28. Akoum A, Lawson C, McColl S, Villeneuve M. Ectopic endometrial cells express high concentrations of interleukin (IL)-8 in vivo regardless of the menstrual cycle phase and respond to oestradiol by up-regulating IL-1-induced IL-8 expression in vitro. Mol Hum Reprod 2001, 7:859-866. 10.1093/molehr/7.9.859, 11517293.
29. Mote PA, Balleine RL, McGowan EM, Clarke CL. Heterogeneity of progesterone receptors A and B expression in human endometrial glands and stroma. Hum Reprod 2000, 15:48-56.
30. Stricker R, Eberhart R, Chevailler MC, Quinn FA, Bischof P. Establishment of detailed reference values for luteinizing hormone, follicle stimulating hormone, estradiol, and progesterone during different phases of the menstrual cycle on the Abbott ARCHITECT analyzer. Clin Chem Lab Med 2006, 44:883-887. 10.1515/CCLM.2006.160, 16776638.
31. Attia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE. Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 2000, 85:2897-2902. 10.1210/jc.85.8.2897, 10946900.
32. Igarashi T, Nakamura Y, Ochiai N, Muto S, Kawamura H, Nakagawa K, Uesato T, Ryo Y. Dioxin may regulate progesterone action and promote development of endometriosis. J Jap Soc Endometriosis 2007, 28:26-32. (in Japanese).