Establishment of a human endometrial cell culture system and characterization of its polarized hormone responsive epithelial cells

Cell and Tissue Research

Volumn 287, Issue 1, 1996, Pages 171-185

  Classen Linke, I ^a   Kusche, M ^b   Knauthe, R ^c   Beier, H M ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

^c BAYER PHARMA AG   (Germany)

Author keywords

Cell culture; Endometrium; Epithelial cells; Hormone receptors; Human; Polarization; Steroid hormones

Indexed keywords

ESTROGEN RECEPTOR; MATRIGEL; MEDROXYPROGESTERONE ACETATE; PROGESTERONE RECEPTOR;

ARTICLE; CELL CULTURE; CELL POLARITY; ENDOMETRIUM CELL; EPITHELIUM CELL; FEMALE; HUMAN; HUMAN CELL; NORMAL HUMAN; PRIORITY JOURNAL;

EID: 0031022722     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004410050743     Document Type: Article

References (55)

1. Bentin-Ley U, Pedersen B, Lindenberg S, Larsen JF, Hamberger L, Horn T (1994) Isolation and culture of human endometrial cells in a three-dimensional culture system. J Reprod Fertil 101: 327-332
2. Bergeron C, Ferenczy A, Toft DO, Schneider W, Shymala G (1988) Immunocytochemical study of progesterone receptors in the human endometrium during the menstrual cycle. Lab Invest 59: 862-869
3. Bergqvist A, Ljungberg O, Skoog L (1993) Immunhistochemical analysis of oestrogen and progesterone receptors in endometriotic tissue and endometrium. Hum Reprod 8: 1915-1922
4. Bissell MJ, Hall HG, Parry G (1982) How does the extracellular matrix direct gene expression? J Theor Biol 99: 31-68
5. Bouchard PH, Marraoui J, Massai MR, Medalie DA, De Ziegler D, Perrot-Applanat M, Frydman, R, Bergeron C (1991) Immuncytochemical localization of estradiol and progesterone receptors in human endometrium: a tool to assess endometrial maturation. Bailliere's Clin Obstet Gynaecol 5: 107-115
6. Carson DD, Tang J-P, Julian JA, Glasser SR (1988) Vectorial secretion of proteoglycans by polarized rat uterine epithelial cells. J Cell Biol 107: 2425-2435
7. Casslen BG, Harper MJK (1991) Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology. Acta Endocrinol (Copenh) 125: 101-108
8. Classen-Linke I, Sohn G, Bolte A, Beier HM (1992) Development of a human endometrial cell culture system. Eur J Cell Biol 57 [Suppl 36]: 14
9. Classen-Linke I, Kusche M, Knauthe R, Beier HM (1995) Expression of progesterone and estrogen receptors in human endometrial cells in culture. Exp Clin Endocrinol Diabetes 103 [Suppl 1]: 113
10. Coppens MT, Dhont MA, De Boever JG, Serreyn RF, Vandekerckhove DA, Roels HJ (1993) The distribution of oestrogen and progesterone receptors in the human endometrial basal and functional layer during the normal menstrual cycle. Histochemistry 99: 121-126
11. Dallenbach-Hellweg G, Poulsen H (1985) Atlas der Histopathologie des Endometriums. Springer, Berlin Heidelberg New York
12. Darcy KM, Black JD, Hahm HA, Ip MM (1991) Mammary organoids from immature virgin rats undergo ductal and alveolar morphogenesis when grown within a reconstituted basement membrane. Exp Cell Res 196: 49-65
13. Emerman JT, Pitelka DR (1977) Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In Vitro 13: 316-328
14. Gerdes J, Schwab U, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13-20
15. Ghosh D, Sengupta J (1995) Morphological characteristics of human endometrial epithelial cells cultured on rat-tail collagen matrix. Hum Reprod 10: 785-790
16. Glasser SR, Julian J, Decker GL, Tang JP, Carson DD (1988) Development of morphological and functional polarity in primary cultures of immature rat uterine epithelial cells. J Cell Biol 107: 2409-2423
17. Hadley MA, Byers SW, Suarez-Quian CA, Kleinman HK, Dym M (1985) Extracellular matrix regulates Sertoli cell differentiation, testicular cord formation, and germ cell development in vitro. J Cell Biol 101: 1511-1522
18. Hadley MA, Djakiew D, Byers SW, Dym M (1987) Polarized secretion of androgen-binding protein and transferrin by Sertoli cells grown in a bicameral culture system. Endocrinology 120: 1097-1103
19. Hohn HP, Winterhager E, Busch LC, Mareel MM, Denker HW (1989) Rabbit endometrium in organ culture: morphological evidence for progestational differentiation in vitro. Cell Tissue Res 257:505-518
20. Hopfer H, Rinehart CA Jr, Vollmer G, Kaufman DG (1994) In vitro interactions of endometrial stromal and epithelial cells in Matrigel: reorganization of the extracellular matrix. Pathobiology 62:104-108
21. Jacobs AL, Decker GL, Glasser SR, Julian J, Carson DD (1990) Vectorial secretion of prostaglandins by polarized rodent uterine epithelial cells. Endocrinology 126: 2125-2136
22. Jacobs AL, Sehgal PB, Julian JA, Carson DD (1992) Secretion and hormonal regulation of interleukin-6 production by mouse uterine stromal and polarized epithelial cells cultured in vitro. Endocrinology 131: 1037-1046
23. Jensen EV, De Sombre ER (1972) Mechanism of action of the female sex hormones. Ann Rev Biochem 41: 203-230
24. Julian JA, Carson DD, Glasser SR (1992) Polarized rat uterine epithelium in vitro: responses to estrogen in defined medium. Endocrinology 130: 68-78
25. Kleinman HK, Mc Garvey ML, Hassell JR, Star VL, Cannon FB, Laurie GW, Martin GR (1986) Basement membrane complexes with biological activity. Biochemistry 25: 312-318
26. Lessey BA, Killam AP, Metzger DA, Haney AF, Greene GL, McCarty S Jr (1988) Immunhistochemical analysis of human uterine estrogen and progesterone receptors throughout the menstrual cycle. J Clin Endocrinol Metab 67:334-340
27. Li ML, Aggeler J, Farson DA, Hatier C, Hassell J, Bissell MJ (1987) Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. Proc Natl Acad Sci USA 84: 136-140
28. Lo HK, Malinin TI, Malini GI (1987) A modified periodic acid-thiocarbohydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J Histochem Cytochem 35: 393-399
29. Mahfoudi A, Nicollier M, Propper AY, Coumes-Marquet S, Adessi GL (1991) Establishment of endometrial glandular epithelial cell subculture in a serum-free, hormonally defined medium, on a basement membrane matrix. Biol Cell 71: 255-265
30. Mahfoudi A, Fauconnet S, Bride J, Beck L, Remy-Martin JP, Nicollier M, Adessi GL (1992) Serum-free culture of stromal and functionally polarized epithelial cells of guines-pig endometrium: a potential model for the study of epithelial-stromal paracrine interactions. Biol Cell 74: 255-265
31. Mahfoudi A, Nicollier M, Beck L, Mularoni A, Cypriani B, Fauconnet S, Adessi GL (1994) Effect of progesterone on proteins vectorially secreted by glandular epithelial cells of guinea-pig endometrium: modulation by homologous stroma. J Reprod Fertil 100: 637-644
32. Mani SK, Decker GL, Glasser SR (1991) Hormonal responsiveness by immature rabbit uterine epithelial cells polarized in vitro. Endocrinology 128: 1563-1573
33. Marshburn PB, Head JR, MacDonald PC, Casey ML (1992) Culture characteristics of human endometrial glandular epithelium throughout the menstrual cycle: modulation of deoxyribonucleic acid synthesis by 17β-estradiol and medroxyprogesterone acetate. Am J Obstet Gynecol 167: 1888-1898
34. Matthews CJ, Redfern CPF, Hirst BH, Thomas EJ (1992) Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture. Fertil Steril 57: 990-997
35. Milgrom E, Baulieu EE (1970) Progesterone in uterus and plasma. I. Binding in rat uterus 105,000 g supernatant. Endocrinology 87: 276-287
36. Milgrom E, Luu T, Atger M, Baulieu EE (1973) Mechanisms regulating the concentration and the conformation of progesterone receptor(s) in the uterus. J Biol Chem 248: 6366-6474
37. Mulholland J, Winterhager E, Beier HM (1988) Changes in proteins synthesized by rabbit endometrial epithelial cells following primary culture. Cell Tissue Res 252: 123-132
38. Negami AI, Tominaga T (1989) Gland and epithelium formation in vitro from epithelial cells of the human endometrium. Hum Reprod 4: 620-624
39. Nisolle M, Casanas-Roux F, Donnez J (1995) Coexpression of cytokeratin and vimentin in eutopic endometrium and endometriosis throughout the menstrual cycle: evaluation by a computerized method. Fertil Steril 64: 69-75
40. Noyes RW, Hertig AT, Rock J (1950) Dating the endometrial biopsy. Fertil Steril 1: 3-25
41. Press MP, Nousek-Goebl N, King WJ, Herbst AL, Greene GJ (1984) Immunhistochemical assessment of estrogen receptor distribution in the human endometrium throughout the menstrual cycle. Lab Invest 51: 495
42. Ravn V, Rasmussen BB, Hojholt L, Barfoed M, Heiberg I, Svenstrup B, Thorpe S (1994) Estrogen-and progesterone receptors in normal cycling endometrium as studied by end-point titration. Cell Tissue Res 276: 419-428
43. Salat-Baroux J, Romain S, Alvarez S, Antoine JM, Kopp K, Raulais D, Brux J de, Martin PM (1994) Biochemical and immunhistochemical multiparametric analysis of steroid receptors and growth factor receptors in human normal endometrium in spontaneous cycles and after the induction of ovulation. Hum Reprod 9: 200-208
44. Schatz F, Gordon RE, Laufer N, Gurpide E (1990) Culture of human endometrial cells under polarizing conditions. Differentiation 42: 184-190
45. Snijders MPML, Goeij AFPM de, Debets-Te Baerts MJC, Rousch MJM, Koudstaal J, Bosman FT (1992) Immuncytochemical analysis of oestrogen receptors and progesterone receptors in the human uterus througout the menstrual cycle and after the menopause. J Reprod Fertil 94: 363-371
46. Spornitz UM (1992) The functional morphology of the human endometrium and decidua. Springer, Berlin Heidelberg New York
47. Streuli CH, Bissell MJ (1990) Expression of extracellular matrix components is regulated by substratum. J Cell Biol 110: 1405-1415
48. Streuli CH, Bailey N, Bissell MJ (1991) Control of mammary epithelial differentiation: basement membrane induces tissue-specific gene expression in the absence of cell-cell interaction and morphological polarity. J Cell Biol 115: 1383-1395
49. Streuli CH, Schmidthauser C, Kobrin M, Bissell MJ, Derynck R (1993) Extracellular matrix regulates expression of the TGF-β1 gene. J Cell Biol 120: 253-260
50. Streuli CH, Schmidthauser C, Bailey N, Yurchenco P, Skubitz APN, Roskelley C, Bissell MJ (1995) Laminin mediates tissue-specific gene expression in mammary epithelia. J. Cell Biol 129: 591-603
51. Tseng L (1979) Physiologic changes in binding and metabolism of estradiol and progesterone in human endometrium during the menstrual cycle. Obstet Gynecol Annu 8: 1-17
52. Tseng L, Gurpide E (1975) Effect of progestins on estradiol receptor levels in human endometrium. J Clin Endocrinol Metab 41: 402
53. Vukicevic S, Kleinman HK, Luyten FP, Roberts AB, Roche NS, Reddi, AH (1992) Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components. Exp Cell Res 202: 1-8
54. White TEK, di Sant'Agnese PA, Miller RK (1990) Human endometrial cells grown on an extracellular matrix form simple columnar epithelia and glands. In Vitro Cell Dev Biol 26: 636-642
55. Winterhager, E, Mulholland J, Glasser SR (1994) Morphological and immunohistochemical differentiation patterns of rabbit uterine epithelium in vitro. Anat Embryol 189: 71-79