Identification and characterization of protein interactions in the mammalian mRNA processing body using a novel two-hybrid assay

Experimental Cell Research

Volumn 317, Issue 15, 2011, Pages 2183-2199

  Bloch, Donald B ^a   Nobre, Rita A ^a   Bernstein, Gillian A ^a   Yang, Wei Hong ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

MRNA degradation; MRNA processing body; Two hybrid assay

Indexed keywords

CELL PROTEIN; MESSENGER RNA; PROTEIN DCP1; PROTEIN DCP2; PROTEIN EDC3; PROTEIN GE1; PROTEIN RAP55; PROTEIN RCK; UNCLASSIFIED DRUG;

ARTICLE; CYTOPLASM; MACROMOLECULE; MAMMAL; MAMMAL CELL; MRNA PROCESSING BODY; NUCLEAR LOCALIZATION SIGNAL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RNA PROCESSING; TWO HYBRID SYSTEM;

ARABIDOPSIS THALIANA; DROSOPHILA MELANOGASTER; MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 79960838430     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2011.05.027     Document Type: Article

References (31)

1. Kedersha N., Anderson P. Mammalian stress granules and processing bodies. Methods Enzymol. 2007, 431:61-81.
2. Eulalio A., Behm-Ansmant I., Izaurralde E. P bodies: at the crossroads of post-transcriptional pathways. Nat. Rev. Mol. Cell Biol. 2007, 8:9-22.
3. Parker R., Sheth U. P bodies and the control of mRNA translation and degradation. Mol. Cell 2007, 25:635-646.
4. Franks T.M., Lykke-Andersen J. The control of mRNA decapping and P-body formation. Mol. Cell 2008, 32:605-615.
5. Brengues M., Teixeira D., Parker R. Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies. Science 2005, 310:486-489.
6. Jakymiw A., Lian S., Eystathioy T., Li S., Satoh M., Hamel J.C., Fritzler M.J., Chan E.K. Disruption of GW bodies impairs mammalian RNA interference. Nat. Cell Biol. 2005, 7:1167-1174.
7. Liu J., Valencia-Sanchez M.A., Hannon G.J., Parker R. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nat. Cell Biol. 2005, 7:719-723.
8. Sheth U., Parker R. Decapping and decay of messenger RNA occur in cytoplasmic processing bodies. Science 2003, 300:805-808.
9. Eulalio A., Behm-Ansmant I., Schweizer D., Izaurralde E. P-body formation is a consequence, not the cause, of RNA-mediated gene silencing. Mol. Cell. Biol. 2007, 27:3970-3981.
10. Teixeira D., Parker R. Analysis of P-Body assembly in Saccharomyces cerevisiae. Mol. Biol. Cell 2007, 18:2274-2287.
11. Goeres D.C., Van Norman J.M., Zhang W., Fauver N.A., Spencer M.L., Sieburth L.E. Components of the Arabidopsis mRNA decapping complex are required for early seedling development. Plant Cell 2007, 19:1549-1564.
12. Xu J., Yang J.Y., Niu Q.W., Chua N.H. Arabidopsis DCP2, DCP1, and VARICOSE form a decapping complex required for postembryonic development. Plant Cell 2006, 18:3386-3398.
13. Fenger-Gron M., Fillman C., Norrild B., Lykke-Andersen J. Multiple processing body factors and the ARE binding protein TTP activate mRNA decapping. Mol. Cell 2005, 20:905-915.
14. Bloch D.B., Rabkina D., Quertermous T., Bloch K.D. The immunoreactive region in a novel autoantigen contains a nuclear localization sequence. Clin. Immunol. Immunopathol. 1994, 72:380-389.
15. Jinek M., Eulalio A., Lingel A., Helms S., Conti E., Izaurralde E. The C-terminal region of Ge-1 presents conserved structural features required for P-body localization. RNA 2008, 14:1991-1998.
16. Haas G., Braun J.E., Igreja C., Tritschler F., Nishihara T., Izaurralde E. HPat provides a link between deadenylation and decapping in metazoa. J. Cell Biol. 2010, 189:289-302.
17. Tritschler F., Braun J.E., Motz C., Igreja C., Haas G., Truffault V., Izaurralde E., Weichenrieder O. DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa. Proc. Natl. Acad. Sci. U.S.A. 2009, 106:21591-21596.
18. Tritschler F., Eulalio A., Helms S., Schmidt S., Coles M., Weichenrieder O., Izaurralde E., Truffault V. Similar modes of interaction enable Trailer Hitch and EDC3 to associate with DCP1 and Me31B in distinct protein complexes. Mol. Cell. Biol. 2008, 28:6695-6708.
19. Yu J.H., Yang W.H., Gulick T., Bloch K.D., Bloch D.B. Ge-1 is a central component of the mammalian cytoplasmic mRNA processing body. RNA 2005, 11:1795-1802.
20. Yang W.H., Yu J.H., Gulick T., Bloch K.D., Bloch D.B. RNA-associated protein 55 (RAP55) localizes to mRNA processing bodies and stress granules. RNA 2006, 12:547-554.
21. Lian S., Fritzler M.J., Katz J., Hamazaki T., Terada N., Satoh M., Chan E.K. Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies. Mol. Biol. Cell 2007, 18:3375-3387.
22. Ferraiuolo M.A., Basak S., Dostie J., Murray E.L., Schoenberg D.R., Sonenberg N. A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay. J. Cell Biol. 2005, 170:913-924.
23. Andrei M.A., Ingelfinger D., Heintzmann R., Achsel T., Rivera-Pomar R., Luhrmann R. A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies. RNA 2005, 11:717-727.
24. Fields S., Sternglanz R. The two-hybrid system: an assay for protein-protein interactions. Trends Genet. 1994, 10:286-292.
25. Yang W.H., Bloch D.B. Probing the mRNA processing body using protein macroarrays and "autoantigenomics". RNA 2007, 13:704-712.
26. Frangioni J.V., Neel B.G. Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem. 1993, 210:179-187.
27. Rihs H.-P., Peters R. Nuclear transport kinetics depend on phosphorylation-site-containing sequences flanking the karyophilic signal of the Simian virus 40T-antigen. EMBO J. 1989, 8:1479-1484.
28. Deshmukh M.V., Jones B.N., Quang-Dang D.U., Flinders J., Floor S.N., Kim C., Jemielity J., Kalek M., Darzynkiewicz E., Gross J.D. mRNA decapping is promoted by an RNA-binding channel in Dcp2. Mol. Cell. 2008, 29:324-336.
29. She M., Decker C.J., Svergun D.I., Round A., Chen N., Muhlrad D., Parker R., Song H. Structural basis of dcp2 recognition and activation by dcp1. Mol. Cell. 2008, 29:337-349.
30. Tritschler F., Eulalio A., Truffault V., Hartmann M.D., Helms S., Schmidt S., Coles M., Izaurralde E., Weichenrieder O. A divergent Sm fold in EDC3 proteins mediates DCP1 binding and P-body targeting. Mol. Cell. Biol. 2007, 27:8600-8611.
31. Harigaya Y., Jones B.N., Muhlrad D., Gross J.D., Parker R. Identification and analysis of the interaction between Edc3 and Dcp2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 2010, 30:1446-1456.