Analysis of differential shrinkage in frozen brain sections and its implications for the use of guard zones in stereology

Journal of Comparative Neurology

Volumn 519, Issue 14, 2011, Pages 2803-2810

  Carlo, C N ^a   Stevens, C F ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

Asymmetrical guard zones; Bias; Compression; Differential shrinkage; Frozen tissue; Guard spaces; Histology; Optical disector; Particle counting; Shrinkage; Stereology

Indexed keywords

ANIMAL TISSUE; ARTICLE; BRAIN ATROPHY; BRAIN SIZE; CONTROLLED STUDY; CRYOPROTECTION; FEMALE; HISTOLOGY; IMMUNOHISTOCHEMISTRY; MALE; MICROTOME; MOUSE; NEUROANATOMY; NONHUMAN; PRIORITY JOURNAL; RAT; SCANNING ELECTRON MICROSCOPY; STEREOLOGY;

ANIMALS; BRAIN; CELL COUNT; FROZEN SECTIONS; MICROTOMY; TISSUE EMBEDDING;

EID: 79960816242     PISSN: 00219967     EISSN: 10969861     Source Type: Journal    
DOI: 10.1002/cne.22652     Document Type: Article

References (21)

1. Baryshnikova LM, Von Bohlen Und Halbach O, Kaplan S, Von Bartheld CS. 2006. Two distinct events, section compression and loss of particles ("lost caps"), contribute to z-axis distortion and bias in optical disector counting. Microsc Res Tech 69: 738-756.
2. Berretta S, Pantazopoulos H, Lange N. 2007. Neuron numbers and volume of the amygdala in subjects diagnosed with bipolar disorder or schizophrenia. Biol Psychiatry 62: 884-893.
3. Bonthius DJ, McKim R, Koele L, Harb H, Karacay B, Mahoney J, Pantazis NJ. 2004. Use of frozen sections to determine neuronal number in the murine hippocampus and neocortex using the optical disector and optical fractionator. Brain Res Brain Res Protoc 14: 45-57.
4. Carlo CN, Stefanacci L, Semendeferi K, Stevens CF. 2010. Comparative analyses of the neuron numbers and volumes of the amygdaloid complex in Old and New World primates. J Comp Neurol 518: 1176-1198.
5. Ciriza I, Carrero P, Azcoitia I, Lundeen SG, Garcia-Segura LM. 2004. Selective estrogen receptor modulators protect hippocampal neurons from kainic acid excitotoxicity: differences with the effect of estradiol. J Neurobiol 61: 209-221.
6. Dombrowski SM, Hilgetag CC, Barbas H. 2001. Quantitative architecture distinguishes prefrontal cortical systems in the rhesus monkey. Cereb Cortex 11: 975-988.
7. Gardella D, Hatton WJ, Rind HB, Rosen GD, von Bartheld CS. 2003. Differential tissue shrinkage and compression in the z-axis: implications for optical disector counting in Vibratome-, plastic- and cryosections. J Neurosci Methods 124: 45-59.
8. Geuna S. 2005. The revolution of counting "tops": two decades of the disector principle in morphological research. Microsc Res Tech 66: 270-274.
9. Gundersen HJ. 1986. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc 143: 3-45.
10. Harding AJ, Halliday GM, Cullen K. 1994. Practical considerations for the use of the optical disector in estimating neuronal number. J Neurosci Methods 51: 83-89.
11. Hatton WJ, von Bartheld CS. 1999. Analysis of cell death in the trochlear nucleus of the chick embryo: calibration of the optical disector counting method reveals systematic bias. J Comp Neurol 409: 169-186.
12. Heaton MB, Mitchell JJ, Paiva M. 2000a. Amelioration of ethanol-induced neurotoxicity in the neonatal rat central nervous system by antioxidant therapy. Alcohol Clin Exp Res 24: 512-518.
13. Heaton MB, Mitchell JJ, Paiva M. 2000b. Overexpression of NGF ameliorates ethanol neurotoxicity in the developing cerebellum. J Neurobiol 45: 95-104.
14. Janiga TA, Rind HB, von Bartheld CS. 2000. Differential effects of the trophic factors BDNF, NT-4, GDNF, and IGF-I on the isthmo-optic nucleus in chick embryos. J Neurobiol 43: 289-303.
15. Mouton PR. 2002. Principles and practices of unbiased stereology: an introduction for bioscientists. Baltimore, MD: The Johns Hopkins University Press.
16. Steljes TP, Kinoshita Y, Wheeler EF, Oppenheim RW, von Bartheld CS. 1999. Neurotrophic factor regulation of developing avian oculomotor neurons: differential effects of BDNF and GDNF. J Neurobiol 41: 295-315.
17. Sterio DC. 1984. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc 134: 127-136.
18. Veiga S, Azcoitia I, Garcia-Segura LM. 2005a. Extragonadal synthesis of estradiol is protective against kainic acid excitotoxic damage to the hippocampus. Neuroreport 16: 1599-1603.
19. Veiga S, Azcoitia I, Garcia-Segura LM. 2005b. Ro5-4864, a peripheral benzodiazepine receptor ligand, reduces reactive gliosis and protects hippocampal hilar neurons from kainic acid excitotoxicity. J Neurosci Res 80: 129-137.
20. Ward TS, Rosen GD, von Bartheld CS. 2008. Optical disector counting in cryosections and Vibratome sections underestimates particle numbers: effects of tissue quality. Microsc Res Tech 71: 60-68.
21. West MJ, Slomianka L, Gundersen HJ. 1991. Unbiased stereological estimation of the total number of neurons in thesubdivisions of the rat hippocampus using the optical fractionator. Anat Rec 231: 482-497.