PCR methods for the rapid detection and identification of four pathogenic Legionella spp. and two Legionella pneumophila subspecies based on the gene amplification of gyrB

Applied Microbiology and Biotechnology

Volumn 91, Issue 3, 2011, Pages 777-787

  Zhou, Guangpeng ^a   Cao, Boyang ^a   Dou, Yan ^a   Liu, Yanwei ^a   Feng, Lu ^a   Wang, Lei ^a  

^a NANKAI UNIVERSITY   (China)

Author keywords

Detection; L. pneumophila; Legionella spp.; PCR; Subspecies

Indexed keywords

16S RRNA; AIR CONDITIONERS; COLONY FORMING UNITS; CONDENSED WATER; GENE AMPLIFICATION; GENE SEQUENCES; GENOMIC DNA; L. PNEUMOPHILA; LEGIONELLA; LEGIONELLA PNEUMOPHILA; LEGIONELLA SPECIES; LEGIONELLA SPP; MULTIPLEX PCR; PCR; PCR METHOD; RAPID DETECTION; SIMULTANEOUS DETECTION; SUBSPECIES; TARGET STRAIN; WATER SAMPLES;

GENE EXPRESSION; MULTIPLEXING; RNA;

POLYMERASE CHAIN REACTION;

BACTERIAL DNA; DNA TOPOISOMERASE (ATP HYDROLYSING) B; RNA 16S;

BACTERIUM; DETECTION METHOD; GENE EXPRESSION; GENETIC ANALYSIS; GENOMICS; IDENTIFICATION METHOD; PATHOGEN; POLYMERASE CHAIN REACTION; SAMPLING; SENSITIVITY ANALYSIS; SUBSPECIES; WATER QUALITY;

AGAR GEL ELECTROPHORESIS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; COLONY FORMING UNIT; CONTROLLED STUDY; GENE AMPLIFICATION; GENE IDENTIFICATION; LEGIONELLA PNEUMOPHILA; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA SEQUENCE; SEQUENCE ANALYSIS;

BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA GYRASE; DNA, BACTERIAL; GENE AMPLIFICATION; LEGIONELLA; LEGIONELLOSIS; LEGIONNAIRES' DISEASE; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TIME FACTORS; WATER MICROBIOLOGY;

CHINA; SHANGHAI;

LEGIONELLA; LEGIONELLA LONGBEACHAE; LEGIONELLA PNEUMOPHILA; LEGIONELLA PNEUMOPHILA SUBSP. FRASERI; LEGIONELLA PNEUMOPHILA SUBSP. PNEUMOPHILA; TATLOCKIA MICDADEI;

EID: 79960711831     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3283-6     Document Type: Article

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