Majority of CD4 + T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule

Proceedings of the National Academy of Sciences of the United States of America

Volumn 108, Issue 27, 2011, Pages 11199-11204

  Josefsson, Lina ^a,b,c   King, Martin S ^d   Makitalo, Barbro ^a   Brännström, Johan ^a   Shao, Wei ^e   Maldarelli, Frank ^c   Kearney, Mary F ^c   Hu, Wei Shau ^c   Chen, Jianbo ^c   Gaines, Hans ^a   Mellors, John W ^f   Albert, Jan ^b   Coffin, John M ^c,g   Palmer, Sarah E ^a,b,c  

^a SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c NATIONAL CANCER INSTITUTE   (United States)

^d ABBOTT LABORATORIES   (United States)

^e SAIC FREDERICK INC   (United States)

^f UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^g TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA;

ARTICLE; CD4+ T LYMPHOCYTE; CLINICAL ARTICLE; COPY NUMBER VARIATION; DNA SEQUENCE; DNA STRUCTURE; GENETIC VARIABILITY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IN VIVO STUDY; PHYLOGENY; PRIORITY JOURNAL; VIRUS RECOMBINATION;

BASE SEQUENCE; CD4-POSITIVE T-LYMPHOCYTES; CHRONIC DISEASE; DNA PRIMERS; DNA, VIRAL; GENETIC VARIATION; HIV INFECTIONS; HIV-1; HUMANS; PHYLOGENY; PROVIRUSES; RECOMBINATION, GENETIC; RNA, VIRAL; SEQUENCE ANALYSIS, DNA; TIME FACTORS; VIRAL LOAD;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 79960569795     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1107729108     Document Type: Article

References (34)

1. Coffin JM (1995) HIV population dynamics in vivo: Implications for genetic variation, pathogenesis, and therapy. Science 267:483-489.
2. Mansky LM, Temin HM (1995) Lower in vivo mutation rate of human immunodeficiency virus type 1 than that predicted from the fidelity of purified reverse transcriptase. J Virol 69:5087-5094.
3. Rouzine IM, Coffin JM (2005) Evolution of human immunodeficiency virus under selection and weak recombination. Genetics 170:7-18. (Pubitemid 40799967)
4. Hu WS, Temin HM (1990) Genetic consequences of packaging two RNA genomes in one retroviral particle: Pseudodiploidy and high rate of genetic recombination. Proc Natl Acad Sci USA 87:1556-1560.
5. Hu WS, Rhodes T, Dang Q, Pathak V (2003) Retroviral recombination: Review of genetic analyses. Front Biosci 8:d143-d155. (Pubitemid 39069969)
6. Basu VP, et al. (2008) Strand transfer events during HIV-1 reverse transcription. Virus Res 134:19-38.
7. Charpentier C, Nora T, Tenaillon O, Clavel F, Hance AJ (2006) Extensive recombination among human immunodeficiency virus type 1 quasispecies makes an important contribution to viral diversity in individual patients. J Virol 80:2472-2482. (Pubitemid 43271553)
8. Li H, et al. (2010) High multiplicity infection by HIV-1 in men who have sex with men. PLoS Pathog 6:e1000890.
9. Nora T, et al. (2007) Contribution of recombination to the evolution of human immunodeficiency viruses expressing resistance to antiretroviral treatment. J Virol 81:7620-7628. (Pubitemid 47047847)
10. Shriner D, Rodrigo AG, Nickle DC, Mullins JI (2004) Pervasive genomic recombination of HIV-1 in vivo. Genetics 167:1573-1583. (Pubitemid 39265897)
11. Chen J, et al. (2005) Mechanisms of nonrandom human immunodeficiency virus type 1 infection and double infection: Preference in virus entry is important but is not the sole factor. J Virol 79:4140-4149. (Pubitemid 40388352)
12. Dang Q, et al. (2004) Nonrandom HIV-1 infection and double infection via direct and cell-mediated pathways. Proc Natl Acad Sci USA 101:632-637. (Pubitemid 38084688)
13. Chen J, et al. (2009) High efficiency of HIV-1 genomic RNA packaging and heterozygote formation revealed by single virion analysis. Proc Natl Acad Sci USA 106:13535-13540.
14. Gratton S, Cheynier R, Dumaurier MJ, Oksenhendler E, Wain-Hobson S (2000) Highly restricted spread of HIV-1 and multiply infected cells within splenic germinal centers. Proc Natl Acad Sci USA 97:14566-14571. (Pubitemid 32016616)
15. Jung A, et al. (2002) Recombination: Multiply infected spleen cells in HIV patients. Nature 418:144. (Pubitemid 34773761)
16. Neher RA, Leitner T (2010) Recombination rate and selection strength in HIV intra-patient evolution. PLOS Comput Biol 6:e1000660.
17. Batorsky R, et al. (2011) Estimate of effective recombination rate and average selection coefficient for HIV in chronic infection. Proc Natl Acad Sci USA 108:5661-5666.
18. Quiñones-Mateu ME, et al. (2002) In vitro intersubtype recombinants of human immunodeficiency virus type 1: Comparison to recent and circulating in vivo recombinant forms. J Virol 76:9600-9613.
19. Simmonds P, et al. (1990) Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. J Virol 64:864-872. (Pubitemid 20032704)
20. Pantaleo G, et al. (1993) HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease. Nature 362:355-358. (Pubitemid 23101310)
21. Nuovo GJ, et al. (1994) In situ detection of PCR-amplified HIV-1 nucleic acids in lymph nodes and peripheral blood in patients with asymptomatic HIV-1 infection and advanced-stage AIDS. J Acquir Immune Defic Syndr 7:916-923.
22. Chen P, Hübner W, Spinelli MA, Chen BK (2007) Predominant mode of human immunodeficiency virus transfer between T cells is mediated by sustained Env-dependent neutralization-resistant virological synapses. J Virol 81:12582-12595. (Pubitemid 350085872)
23. Psallidopoulos MC, et al. (1989) Integrated proviral human immunodeficiency virus type 1 is present in CD4+ peripheral blood lymphocytes in healthy seropositive individuals. J Virol 63:4626-4631. (Pubitemid 19257837)
24. Brinchmann JE, Albert J, Vartdal F (1991) Few infected CD4+ T cells but a high proportion of replication-competent provirus copies in asymptomatic human immunodeficiency virus type 1 infection. J Virol 65:2019-2023.
25. Schnittman SM, et al. (1990) Increasing viral burden in CD4+ T cells from patients with human immunodeficiency virus (HIV) infection reflects rapidly progressive immunosuppression and clinical disease. Ann Intern Med 113:438-443. (Pubitemid 20333452)
26. Ellery PJ, et al. (2007) The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 in vivo. J Immunol 178:6581-6589. (Pubitemid 46717442)
27. Gartner S, et al. (1986) The role of mononuclear phagocytes in HTLV-III/LAV infection. Science 233:215-219.
28. Fulcher JA, et al. (2004) Compartmentalization of human immunodeficiency virus type 1 between blood monocytes and CD4+ T cells during infection. J Virol 78:7883-7893. (Pubitemid 38944274)
29. Perelson AS, Neumann AU, Markowitz M, Leonard JM, Ho DD (1996) HIV-1 dynamics in vivo: Virion clearance rate, infected cell life-span, and viral generation time. Science 271:1582-1586. (Pubitemid 26097216)
30. Edo-Matas D, et al. (2010) Genetic composition of replication competent clonal HIV-1 variants isolated from peripheral blood mononuclear cells (PBMC), HIV-1 proviral DNA from PBMC and HIV-1 RNA in serum in the course of HIV-1 infection. Virology 405:492-504.
31. Kearney M, et al. (2009) Human immunodeficiency virus type 1 population genetics and adaptation in newly infected individuals. J Virol 83:2715-2727.
32. Keele BF, et al. (2008) Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proc Natl Acad Sci USA 105:7552-7557. (Pubitemid 351830056)
33. Palmer S, et al. (2005) Multiple, linked human immunodeficiency virus type 1 drug resistance mutations in treatment-experienced patients are missed by standard genotype analysis. J Clin Microbiol 43:406-413. (Pubitemid 40096331)
34. Kearney M, et al. (2008) Frequent polymorphism at drug resistance sites in HIV-1 protease and reverse transcriptase. AIDS 22:497-501. (Pubitemid 351317291)