Immobilization of aptamers onto unmodified glass surfaces for affordable biosensors

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 8034, Issue , 2011, Pages

  Chen, Rui ^a   Surman, Cheryl ^a   Potyrailo, Radislav ^a   Pris, Andrew ^a   Holwitt, Eric A ^b   Sorola, Veronica K ^b   Kiel, Johnathan L ^b  

^a GENERAL ELECTRIC COMPANY   (United States)

^b AIR FORCE RESEARCH LABORATORY   (United States)

Author keywords

Aptamer; Immobilization; Poly T; Sensor; Silicon dioxide wafer; Unmodified glass; UV crosslinking

Indexed keywords

APTAMER SENSOR; APTAMERS; BIORECEPTORS; DYNAMIC RANGE; FUNCTIONALIZATIONS; G-QUADRUPLEXES; GLASS SURFACES; LOW COSTS; POLY T; SENSOR SURFACES; STREPTAVIDIN; TARGET BINDING; UNMODIFIED GLASS; UV-CROSSLINKING;

BIOSENSORS; DNA SEQUENCES; PROTEINS; SEMICONDUCTING SILICON COMPOUNDS; SENSORS; SILICA; SILICON OXIDES; SILICON WAFERS;

GLASS;

EID: 79960551800     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.883384     Document Type: Conference Paper

References (19)

1. Potyrailo, R. A., Conrad, R. C., Ellington, A. D., and Hieftje, G. M., "Adapting Selected Nucleic Acid Ligands (Aptamers) to Biosensors," Anal. Chem., 70(16), 3419 (1998).
2. Bruno, J. G., and Kiel, J. L., "In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detection," Biosens. Bioelectron., 14(5), 457 (1999).
3. Balamurugan, S., Obubuafo, A., Soper, S. A., and Spivak, D. A., "Surface immobilization methods for aptamer diagnostic applications," Anal. Bioanal. Chem., 390(4), 1009 (2008).
4. Jayasena, S. D., "Aptamers: An emerging class of molecules that rival antibodies in diagnostics," Clin. Chem., 45(9), 1628-1650 (1999). (Pubitemid 29419030)
5. Chiu, T. C., and Huang, C. C., "Aptamer-functionalized nano-biosensors," Sensors, 9(12), 10356-10388 (2009).
6. Fischer, N. O., Tarasow, T. M., and Tok, J. B. H., "Aptasensors for biosecurity applications," Curr. Opin. Chem. Biol., 11(3), 316 (2007).
7. Gudnason, H., Dufva, M., Bang, D. D., and Wolff, A., "An inexpensive and simple method for thermally stable immobilization of DNA on an unmodified glass surface: UV linking of poly(T)10-poly(C)10-tagged DNA probes," BioTechniques, 45(3), 261-271 (2008).
8. Obubuafo, A., Balamurugan, S., Shadpour, H., Spivak, D., McCarley, R. L., and Soper, S. A., "Poly(methyl methacrylate) microchip affinity capillary gel electrophoresis of aptamer-protein complexes for the analysis of thrombin in plasma," Electrophoresis, 29(16 SPEC. ISS.), 3436-3445 (2008).
9. Battaglia, C., Salani, G., Consolandi, C., Bernardi, L. R., and Bellis, G. D., "Analysis of DNA Microarrays by Non-Destructive Fluorescent Staining Using SYBR® Green II," BioTechniques, 29(1), 78-81 (2000). (Pubitemid 30439419)
10. Walter, J. G., Kökpinar, Ö., Friehs, K., Stahl, F., and Scheper, T. "Systematic investigation of optimal aptamer immobilization for protein-microarray applications," Anal. Chem., 80(19), 7372-7378 (2008).
11. Görner, H., "New trends in photobiology: Photochemistry of DNA and related biomolecules: Quantum yields and consequences of photoionization," J. Photochem. Photobiol. B Biol., 26(2), 117-139 (1994).
12. Li, J. J., Fang, X., and Tan, W., "Molecular aptamer beacons for real-time protein recognition," Biochem. Biophys. Res. Commun., 292(1), 31 (2002).
13. Hamaguchi, N., Ellington, A., and Stanton, M., "Aptamer beacons for the direct detection of proteins," Anal. Biochem., 294(2), 126 (2001).
14. Tasset, D. M., Kubik, M. F., and Steiner, W., "Oligonucleotide inhibitors of human thrombin that bind distinct epitopes," J. Mol. Biol., 272(5), 688 (1997).
15. Ostatna, V., Vaisocherova, H., Homola, J., and Hianik, T., "Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance," Anal. Bioanal. Chem., 391(5), 1861 (2008).
16. Lee, M., and Walt, D. R., "A fiber-optic microarray biosensor using aptamers as receptors," Anal. Biochem., 282(1), 142 (2000).
17. Howarter, J. A., and Youngblood, J. P., "Optimization of silica silanization by 3-aminopropyltriethoxysilane," Langmuir, 22(26), 11142-11147 (2006). (Pubitemid 46129653)
18. Pasternack, R. M., Amy, S. R., and Chabal, Y. J., "Attachment of 3-(aminopropyl)triethoxysilane on silicon oxide surfaces: Dependence on solution temperature," Langmuir, 24(22), 12963-12971 (2008).
19. Bras, M., Dugas, V., Bessueille, F., Cloarec, J. P., Martin, J. R., Cabrera, M., Chauvet, J. P., Souteyrand, E., and Garrigues, M., "Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray," Biosens. Bioelectron., 20(4), 796-805 (2004). (Pubitemid 39441259)