Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR® green II

BioTechniques

Volumn 29, Issue 1, 2000, Pages 78-81

  Battaglia, C ^a   Salani, G ^a   Consolandi, C ^a   Rossi Bernardi, L ^a   De Bellis, Gianluca ^a  

^a CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA DENATURATION; DNA DETERMINATION; FLUORESCENCE; GENE TARGETING; QUALITY CONTROL; STAINING;

EID: 0033939062     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00291st01     Document Type: Article

References (12)

1. Beier, M. and J.D. Hoheisel. 1999. Versatile derivatisation of solid support media for covalent bonding on DNA-microchips. Nucleic Acids Res. 27:1970-1977.
2. Brown, P.O. and D. Botstein. 1999. Exploring the new world of the genome with DNA microarrays. Nat. Genet. 21:33-37.
3. Drobyshev, A., N. Mologina, V. Shik, D. Pobedimskaya, G. Yershov and A. Mirzabekov. 1997. Sequence analysis by hybridization with oligonucleotide microchip: identification of beta-thalassemia mutations. Gene 188:45-52.
4. Eisen, M.B. and P.O. Brown. 1999. DNA arrays for analysis of gene expression. Methods Enzymol. 303:179-205.
5. Lander, E.S. 1999. Array of hope. Nat. Genet. 27:3-4.
6. Maskos U. and E.M. Southern. 1992. Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ. Nucleic Acids Res. 20:1679-1684.
7. Molecular Probes SYBR Green II Product Information Sheet.
8. Pease, A.C., D. Solas, E.J. Sullivan, M.T. Cronin, C.P. Holmes and S.P. Fodor. 1994. Light-generated Oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA 91:5022-5026.
9. Pietu, G., R. Mariage-Samson, N.A. Fayein, C. Matingou, E. Eveno, R. Houlgatte, C. Decraene, Y. Vandenbrouck et al. 1999. The Genexpress IMAGE knowledge base of the human brain transcriptome: a prototype integrated resource for functional and computational genomics. Genome Res. 9:195-209.
10. Ramsay, G. 1998. DNA chips: state-of-the art. Nat. Biotechnol. 16:40-44.
11. Schena, M., D. Shalon, R.W. Davis and P.O. Brown. 1995. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467-470.
12. Schena, M., D. Shalon, R. Heller, A. Chai, P.O. Brown and R.W. Davis. 1996. Parallel human genome analysis-microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA 93:10614-10619.