A flexible bioreactor system for constructing in vitro tissue and organ models

Biotechnology and Bioengineering

Volumn 108, Issue 9, 2011, Pages 2129-2140

  Vozzi, Federico ^a   Mazzei, Daniele ^b   Vinci, Bruna ^b   Vozzi, Giovanni ^b   Sbrana, Tommaso ^b   Ricotti, Leonardo ^b   Forgione, Nicola ^b   Ahluwalia, Arti ^a,b  

^a INSTITUTE OF CLINICAL PHYSIOLOGY   (Italy)

^b UNIVERSITY OF PISA   (Italy)

Author keywords

Bioreactor; Cell testing; Connected cell culture; Fluid dynamic simulation; Laminar flow; Multi compartmental chamber

Indexed keywords

BIOREACTOR SYSTEM; CELL TESTING; CONNECTED CELL CULTURE; CULTURE CHAMBERS; DESIGN PHILOSOPHY; FLUID-DYNAMIC SIMULATION; HEPATOCYTES; IN-VITRO; IN-VIVO; METABOLIC FUNCTION; MULTI-COMPARTMENTAL CHAMBER; USER FRIENDLY; VALIDATION TEST;

BIOCONVERSION; CELL CULTURE; COMPUTER SIMULATION; ENDOTHELIAL CELLS; LAMINAR FLOW; TISSUE;

BIOREACTORS;

ANIMAL CELL; ARTICLE; BIOREACTOR; BIOREACTOR DESIGN; CELL METABOLISM; CONTROLLED STUDY; ENDOTHELIUM CELL; IN VITRO STUDY; LAMINAR FLOW; LAMINAR FLOW CHAMBER; LIVER CELL; MALE; MODULAR MULTI COMPARTMENTAL CHAMBER; MULTICOMPARTMENTAL CHAMBER; NONHUMAN; PHILOSOPHY; SHEAR STRESS; TISSUE ENGINEERING; VALIDATION PROCESS;

ANIMALS; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELLS, CULTURED; ENDOTHELIAL CELLS; EQUIPMENT DESIGN; HEPATOCYTES; HUMANS; MALE; MICE; MICE, INBRED C57BL; MODELS, BIOLOGICAL; REPRODUCIBILITY OF RESULTS; SHEAR STRENGTH;

EID: 79960452349     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23164     Document Type: Article

References (39)

1. Basu R, Camillo BD, Toffolo G, Basu A, Shah P, Vella A, Rizza R, Cobelli C. 2003. Use of a novel triple tracer approach to assess postprandial glucose metabolism. Am J Physiol Endocrinol Metab 284: E55.
2. Caro CG. 2009. Discovery of the role of wall shear in atherosclerosis. Arterioscler Thromb Vasc Biol 29: 158.
3. Caro CG, Pedley TJ, Schroter RC, Seed WA. 1978. The Mechanics of the Circulation. Oxford medical publications, New York City, Oxford University Press.
4. Chen Y. 1992. A perfusion system for high productivity of monoclonal antibody by hybridoma cells in a CelliGen bioreactor. Chin J Biotechnol 8(3): 179-186.
5. De Bartolo L, Salerno S, Morelli S, Giorno L, Rende M, Memoli B, Procino A, Andreucci VE, Bader A, Drioli E. 2006. Long-term maintenance of human hepatocytes in oxygen-permeable membrane bioreactor. Biomaterials 27(27): 4794-4803 [Epub 2006 June 6].
6. Fung YC. 1997. Biomechanics: Circulation. 2nd edition. New York: Springer.
7. Glockner PS, Naterer GF. 2005. Near-wall velocity profile with adaptive shape functions for turbulent forced convection. Int Commun Heat Mass Transf 32(1-2): 72-79.
8. Graber R, Sumida C, Nunez EA. 1994. Fatty acids and cell signal transduction. J Lipid Mediat Cell Signal 9(2): 91-9116. Review.
9. Guzzardi MA, Domenici C, Ahluwalia A. Metabolic control through hepatocyte and adipose tissue cross-talk in a multicompartmental modular bioreactor. Tissue Eng Part A 2011.
10. Henson MA, Seborg DE. 1994. Adaptive nonlinear control of a pH neutralization process. IEEE Trans Control Syst Technol 2(3): 169-182.
11. Hsieh MH, Nguyen HT. 2005. Molecular mechanism of apoptosis induced by mechanical forces. Int Rev Cytol 245: 45-90. Review.
12. Kiefer F, Vogel WF, Arnold R. 2002. Signal transduction and co-stimulatory pathways. Transpl Immunol 9(2-4): 69-82. Review.
13. Kim DW, Gotlieb AI, Langille BL. 1989. In vivo modulation of endothelial F-actin microfilaments by experimental alterations in shear stress. Arterioscler Thromb Vasc Biol 9: 439-445.
14. Kuchan MJ, Frangos JA. 1993. Shear stress regulates endothelin-1 release via protein kinase C and cGMP in cultured endothelial cells. Am J Physiol 264(1 Pt 2): H150-H156.
15. Lee HL, Boccazzi P, Ram RJ, Sinskey AJ. 2006. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip 6(9): 1229-1235 [Epub 2006 July 27].
16. Malek AM, Greene AL, Izumo S. 1993. Regulation of endothelin 1 gene by fluid shear stress is transcriptionally mediated and independent of protein kinase C and cAMP. Proc Natl Acad Sci USA 90(13): 5999-6003.
17. Mazzei D, Guzzardi MA, Giusti S, Ahluwalia A. 2001. A low shear stress modular bioreactor for connected cell culture under high flow rates. Biotechnol Bioeng 106(1): 127-137.
18. McDonald JC, Whitesides GM. 2002. Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res 35(7): 491-499. Review.
19. McMahon SB, Monroe JG. 1992. Role of primary response genes in generating cellular responses to growth factors. FASEB J 6(9): 2707-2715. Review.
20. Mercille S, Johnson M, Lanthier S, Kamen AA, Massie B. 2000. Understanding factors that limit the productivity of suspension-based perfusion cultures operated at high medium renewal rates. Biotechnol Bioeng 67(4): 435-450.
21. Myers KA, Rattner JB, Shrive NG, Hart DA. 2007. Hydrostatic pressure sensation in cells: Integration into the tensegrity model. Biochem Cell Biol 85(5): 543-551. Review.
22. Powers MJ, Janigian DM, Wack KE, Baker CS, Beer Stolz D, Griffith LG. 2002. Functional behavior of primary rat liver cells in a three-dimensional perfused microarray bioreactor. Tissue Eng 8(3): 499-513.
23. Prior BM, Yang HT, Terjung RL. 2004. What makes vessels grow with exercise training? J Appl Physiol 97(3): 1119-1128. Review.
24. Reuss M. 1995. Stirred tank bioreactors. Bioprocess Technol 21: 207-255. Review.
25. Santarelli MF, Sani L, Ahluwalia A, Vozzi G, Landini L, De Rossi D. 2003. A new method for quantitative cellular imaging on 3-D scaffolds using fluorescence microscopy. IEEE Trans Nanobiosci 2(2): 110.
26. Saxena R, Pan G, McDonald JM. 2007. Osteoblast and osteoclast differentiation in modeled microgravity. Ann NY Acad Sci 1116: 494-498. Review.
27. Schmalzriedt S, Jenne M, Mauch K, Reuss M. 2003. Integration of physiology and fluid dynamics. Adv Biochem Eng Biotechnol 80: 19-68. Review.
28. Schwartlander R, Schmid J, Brandenburg B, Katenz E, Vondran FW, Pless G, Cheng X, Pascher A, Neuhaus P, Sauer IM. 2007. Continuously microscopically observed and process-controlled cell culture within the SlideReactor: Proof of a new concept for cell characterization. Tissue Eng 13(1): 187-196.
29. Sharefkin JB, Diamond SL, Eskin SG, McIntire LV, Dieffenbach CW. 1991. Fluid flow decreases preproendothelin mRNA levels and suppresses endothelin-1 peptide release in cultured human endothelial cells. J Vasc Surg 14(1): 1-9.
30. Sung JH, Shuler ML. 2009. A micro cell culture analog (microCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs. Lab Chip 9(10): 1385-1394.
31. Vinci B, Cavallone D, Vozzi G, Mazzei D, Domenici C, Brunetto M, Ahluwalia A. 2010a. In vitro liver model using microfabricated scaffolds in a modular bioreactor. Biotechnol J 5(2): 232-241.
32. Vinci B, Murphy E, Iori E, Marescotti MC, Avogaro A, Ahluwalia A. 2010b. Flow-regulated glucose and lipid metabolism in adipose tissue, endothelial cell and hepatocyte cultures in a modular bioreactor. Biotechnol J 5(6): 618-626.
33. Vinci B, Duret C, Klieber S, Gerbal-Chaloin S, Sa-Cunha A, Laporte S, Suc B, Maurel P, Ahluwalia A, Daujat-Chavanieu M. 2011. Medium flow stimulates expression and activity of detoxification genes in primary human hepatocytes cultured in a multicompartment modular bioreactor. Biotechnol J DOI: 10.1002/biot.20100326.
34. Vinci B, Murphy E, Iori E, Meduri F, Fattori S, Marescotti MC, Castagna M, Avogaro A, Ahluwalia A. Submitted. An in-vitro model of metabolism connecting adipose tissue, endothelial cells and hepatocytes in a multicompartmental bioreactor. Biotechnology J.
35. Vozzi G, Previti A, De Rossi D, Ahluwalia A. 2002. Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering. Tissue Eng 8(6): 1089-1098.
36. Vozzi F, Heinrich JM, Bader A, Ahluwalia A. 2009. Connected culture of murine hepatocytes and human umbilical vein endothelial cells in a multicompartmental bioreactor. Tissue Eng Part A 15(6): 1291-1299.
37. Wang D, Liu W, Han B, Xu R. 2005. The bioreactor: A powerful tool for large-scale culture of animal cells. Curr Pharm Biotechnol 6(5): 397-403. Review.
38. West GB, Brown JH, Enquist BJ. 1997. A general model for the origin of allometric scaling laws in biology. Science 276(5309): 122-126.
39. Ye H, Xia Z, Ferguson DJ, Triffitt JT, Cui Z. 2007. Studies on the use of hollow fibre membrane bioreactors for tissue generation by using rat bone marrow fibroblastic cells and a composite scaffold. J Mater Sci Mater Med 18(4): 641-648.