Identification of amino acid residues critical for catalysis of holliday junction resolution by Mycoplasma genitalium RecU

Journal of Bacteriology

Volumn 193, Issue 15, 2011, Pages 3941-3948

  Sluijter, Marcel ^a   Aslam, Mohammad ^a   Hartwig, Nico G ^a   van Rossum, Annemarie M C ^a   Vink, Cornelis ^a  

^a SOPHIA CHILDREN S HOSPITAL   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

RECU PROTEIN; RESOLVASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CATALYSIS; DELETION MUTANT; ENZYME SUBSTRATE; GEOBACILLUS STEAROTHERMOPHILUS; HOLLIDAY JUNCTION; MYCOPLASMA GENITALIUM; MYCOPLASMA PENETRANS; MYCOPLASMA PNEUMONIAE; MYCOPLASMA PULMONIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CLEAVAGE; UREAPLASMA; UREAPLASMA PARVUM; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; BIOCATALYSIS; DNA, CRUCIFORM; HOLLIDAY JUNCTION RESOLVASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MYCOPLASMA GENITALIUM; PROTEIN BINDING; SEQUENCE ALIGNMENT;

BACTERIA (MICROORGANISMS); MYCOPLASMA GENITALIUM;

EID: 79960400933     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00247-11     Document Type: Article

References (28)

1. Ayora, S., B. Carrasco, E. Doncel-Perez, R. Lurz, and J. C. Alonso. 2004. Bacillus subtilis RecU protein cleaves Holliday junctions and anneals singlestranded DNA. Proc. Natl. Acad. Sci. U. S. A. 101:452-457.
2. Burgos, R., et al. 2006. Mycoplasma genitalium P140 and P110 cytadhesins are reciprocally stabilized and required for cell adhesion and terminal-organelle development. J. Bacteriol. 188:8627-8637.
3. Canas, C., B. Carrasco, S. Ayora, and J. C. Alonso. 2008. The RecU Holliday junction resolvase acts at early stages of homologous recombination. Nucleic Acids Res. 36:5242-5249.
4. Carrasco, B., C. Canas, G. J. Sharples, J. C. Alonso, and S. Ayora. 2009. The N-terminal region of the RecU Holliday junction resolvase is essential for homologous recombination. J. Mol. Biol. 390:1-9.
5. Chan, S. N., L. Harris, E. L. Bolt, M. C. Whitby, and R. G. Lloyd. 1997. Sequence specificity and biochemical characterization of the RusA Holliday junction resolvase of Escherichia coli. J. Biol. Chem. 272:14873-14882.
6. Clausen, H. F., et al. 2001. Serological investigation of Mycoplasma genitalium in infertile women. Hum. Reprod. 16:1866-1874.
7. Dallo, S. F., and J. B. Baseman. 1991. Adhesin gene of Mycoplasma genitalium exists as multiple copies. Microb. Pathog. 10:475-480.
8. Dhandayuthapani, S., W. G. Rasmussen, and J. B. Baseman. 1999. Disruption of gene mg218 of Mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype. Proc. Natl. Acad. Sci. U. S. A. 96:5227-5232.
9. Dhandayuthapani, S., W. G. Rasmussen, and J. B. Baseman. 2002. Stability of cytadherence-related proteins P140/P110 in Mycoplasma genitalium requires MG218 and unidentified factors. Arch. Med. Res. 33:1-5.
10. Fraser, C. M., et al. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
11. Guex, N., and M. C. Peitsch. 1997. SWISS-MODEL and the Swiss-Pdb-Viewer: an environment for comparative protein modeling. Electrophoresis 18:2714-2723.
12. Hu, P. C., et al. 1987. A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein. Infect. Immun. 55:1126-1131.
13. Ingleston, S. M., et al. 2002. Holliday junction binding and processing by the RuvA protein of Mycoplasma pneumoniae. Eur. J. Biochem. 269:1525-1533.
14. Iverson-Cabral, S. L., S. G. Astete, C. R. Cohen, E. P. Rocha, and P. A. Totten. 2006. Intrastrain heterogeneity of the mgpB gene in Mycoplasma genitalium is extensive in vitro and in vivo and suggests that variation is generated via recombination with repetitive chromosomal sequences. Infect. Immun. 74:3715-3726.
15. Iverson-Cabral, S. L., S. G. Astete, C. R. Cohen, and P. A. Totten. 2007. mgpB and mgpC sequence diversity in Mycoplasma genitalium is generated by segmental reciprocal recombination with repetitive chromosomal sequences. Mol. Microbiol. 66:55-73.
16. Kelley, L. A., and M. J. Sternberg. 2009. Protein structure prediction on the Web: a case study using the Phyre server. Nat. Protoc. 4:363-371.
17. Kelly, S. J., J. Li, P. Setlow, and M. J. Jedrzejas. 2007. Structure, flexibility, and mechanism of the Bacillus stearothermophilus RecU Holliday junction resolvase. Proteins 68:961-971.
18. McGregor, N., et al. 2005. The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage. Structure 13:1341-1351.
19. Peterson, S. N., et al. 1995. Characterization of repetitive DNA in the Mycoplasma genitalium genome: possible role in the generation of antigenic variation. Proc. Natl. Acad. Sci. U. S. A. 92:11829-11833.
20. Peterson, S. N., P. C. Hu, K. F. Bott, and C. A. Hutchison III. 1993. A survey of the Mycoplasma genitalium genome by using random sequencing. J. Bacteriol. 175:7918-7930.
21. Rigden, D. J., et al. 2002. PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA. Protein Sci. 11:2370-2381.
22. Shah, R., R. J. Bennett, and S. C. West. 1994. Activation of RuvC Holliday junction resolvase in vitro. Nucleic Acids Res. 22:2490-2497.
23. Shah, R., R. J. Bennett, and S. C. West. 1994. Genetic recombination in E. coli: RuvC protein cleaves Holliday junctions at resolution hotspots in vitro. Cell 79:853-864.
24. Sharples, G. J., S. N. Chan, A. A. Mahdi, M. C. Whitby, and R. G. Lloyd. 1994. Processing of intermediates in recombination and DNA repair: identification of a new endonuclease that specifically cleaves Holliday junctions. EMBO J. 13:6133-6142.
25. Sluijter, M., T. Hoogenboezem, N. G. Hartwig, and C. Vink. 2008. The Mycoplasma pneumoniae MPN229 gene encodes a protein that selectively binds single-stranded DNA and stimulates Recombinase A-mediated DNA strand exchange. BMC Microbiol. 8:167.
26. Sluijter, M., et al. 2010. The Mycoplasma genitalium MG352-encoded protein is a Holliday junction resolvase that has a non-functional orthologue in Mycoplasma pneumoniae. Mol. Microbiol. 77:1261-1277.
27. Sluijter, M., E. B. Spuesens, N. G. Hartwig, A. M. van Rossum, and C. Vink. 2009. The Mycoplasma pneumoniae MPN490 and Mycoplasma genitalium MG339 genes encode RecA homologs that promote homologous DNA strand exchange. Infect. Immun. 77:4905-4911.
28. Svenstrup, H. F., J. S. Jensen, K. Gevaert, S. Birkelund, and G. Christiansen. 2006. Identification and characterization of immunogenic proteins of Mycoplasma genitalium. Clin. Vaccine Immunol. 13:913-922.