Mycoplasma genitalium P140 and P110 cytadhesins are reciprocally stabilized and required for cell adhesion and terminal-organelle development

Journal of Bacteriology

Volumn 188, Issue 24, 2006, Pages 8627-8637

  Burgos, Raul ^a   Pich, Oscar Q ^a   Ferrer Navarro, Mario ^a   Baseman, Joel B ^b   Querol, Enrique ^a   Piñol, Jaume ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b Mays Cancer Center at UT Health San Antonio   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; BACTERIAL PROTEIN; CYTOSKELETON PROTEIN; MUTANT PROTEIN; PROTEIN DNAK; PROTEIN MG386; PROTEIN P110; PROTEIN P140;

ANTIGENIC VARIATION; ARTICLE; BACTERIAL GENE; BACTERIAL GENETICS; BACTERIUM ADHERENCE; CELL ORGANELLE; CORRELATION ANALYSIS; CYTOSKELETON; GENE DELETION; GENE MUTATION; GENETIC COMPLEMENTATION; GENETIC RECOMBINATION; MG191 GENE; MG192 GENE; MYCOPLASMA GENITALIUM; NONHUMAN; OPERON; PHENOTYPE; PRIORITY JOURNAL; PROTEIN STABILITY; SCANNING ELECTRON MICROSCOPY;

ADHESINS, BACTERIAL; ANTIGENIC VARIATION; BACTERIAL ADHESION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; HUMANS; MUTATION; MYCOPLASMA GENITALIUM; ORGANELLES; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINATION, GENETIC; TRANSFORMATION, BACTERIAL;

MYCOPLASMA GENITALIUM;

EID: 33845449791     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00978-06     Document Type: Article

References (49)

1. Baseman, J. B. 1993. The cytadhesins of Mycoplasma pneumoniae and M. genitalium. Subcell. Biochem. 20:243-259.
2. Baseman, J. B., M. Cagle, J. E. Korte, C. Herrera, W. G. Rasmussen, J. G. Baseman, R. Shain, and J. M. Piper. 2004. Diagnostic assessment of Mycoplasma genitalium in culture-positive women. J. Clin. Microbiol. 42:203-211.
3. Baseman, J. B., R. M. Cole, D. C. Krause, and D. K. Leith. 1982. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J. Bacteriol. 151:1514-1522.
4. Baseman, J. B., S. F. Dallo, J. G. Tully, and D. L. Rose. 1988. Isolation and characterization of Mycoplasma genitalium strains from the human respiratory tract. J. Clin. Microbiol. 26:2266-2269.
5. Baseman, J. B., and J. G. Tully. 1997. Mycoplasmas: sophisticated, reemerging, and burdened by their notoriety. Emerg. Infect. Dis. 3:21-32.
6. Dallo, S. F., A. Chavoya, C. J. Su, and J. B. Baseman. 1989. DNA and protein sequence homologies between the adhesins of Mycoplasma genitalium and Mycoplasma pneumoniae. Infect. Immun. 57:1059-1065.
7. Dhandayuthapani, S., M. W. Blaylock, C. M. Bebear, W. G. Rasmussen, and J. B. Baseman. 2001. Peptide methionine sulfoxide reductase (MsrA) is a virulence determinant in Mycoplasma genitalium. J. Bacteriol. 183:5645-5650.
8. Dhandayuthapani, S., W. G. Rasmussen, and J. B. Baseman. 1999. Disruption of gene mg218 of Mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype. Proc. Natl. Acad. Sci. USA 96:5227-5232.
9. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. CIayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, R. D. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J. F. Tomb, B. A. Dougherty, K. F. Bott, P. C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C. A. Hutchison III, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
10. Giron, J. A., M. Lange, and J. B. Baseman. 1996. Adherence, fibronectin binding, and induction of cytoskeleton reorganization in cultured human cells by Mycoplasma penetrans. Infect. Immun. 64:197-208.
11. Glass, J. I., N. Assad-Garcia, N. Alperovich, S. Yooseph, M. R. Lewis, M. Maruf, C. A. Hutchison III, H. O. Smith, and J. C. Venter. 2006. Essential genes of a minimal bacterium. Proc. Natl. Acad. Sci. USA 103:425-430.
12. Henderson, I. R., P. Owen, and J. P. Nataro. 1999. Molecular switches-the ON and OFF of bacterial phase variation. Mol. Microbiol. 33:919-932.
13. Horner, P. J., C. B. Gilroy, B. J. Thomas, R. O. Naidoo, and D. Taylor-Robinson. 1993. Association of Mycoplasma genitalium with acute non-gonococcal urethritis. Lancet 342:582-585.
14. Hutchison, C. A., S. N. Peterson, S. R. Gill, R. T. Cline, O. White, C. M. Fraser, H. O. Smith, and J. C. Venter. 1999. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286:2165-2169.
15. Iverson-Cabral, S. L., S. G. Astete, C. R. Cohen, E. P. Rocha, and P. A. Totten. 2006. Intrastrain heterogeneity of the mgpB gene in Mycoplasma genitalium is extensive in vitro and in vivo and suggests that variation is generated via recombination with repetitive chromosomal sequences. Infect. Immun. 74:3715-3726.
16. Krause, D. C., and M. F. Balish. 2004. Cellular engineering in a minimal microbe: structure and assembly of the terminal organelle of Mycoplasma pneumoniae. Mol. Microbiol. 51:917-924.
17. Krause, D. C., D. K. Leith, R. M. Wilson, and J. B. Baseman. 1982. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence. Infect. Immun. 35:809-817.
18. Layh-Schmitt, G., and M. Harkenthal. 1999. The 40- and 90-kDa membrane proteins (ORF6 gene product) of Mycoplasma pneumoniae are responsible for the tip structure formation and P1 (adhesin) association with the Triton shell. FEMS Microbiol. Lett. 174:143-149.
19. Layh-Schmitt, G., and R. Herrmann. 1992. Localization and biochemical characterization of the ORF6 gene product of the Mycoplasma pneumoniae P1 operon. Infect. Immun. 60:2906-2913.
20. Layh-Schmitt, G., A. Podtelejnikov, and M. Mann. 2000. Proteins complexed to the P1 adhesin of Mycoplasma pneumoniae. Microbiology 146: 741-747.
21. Luo, D., W. Xu, G. Liang, S. Wang, Z. Wang, Z. Bi, and W. Zhu. 1999. Isolation and identification of Mycoplasma genitalium from high risk populations of sexually transmitted diseases in China. Chin. Med. J. 112:489-492.
22. Lysnyansky, I., Y. Ron, K. Sachse, and D. Yogev. 2001. Intrachromosomal recombination within the vsp locus of Mycoplasma bovis generates a chimeric variable surface lipoprotein antigen. Infect. Immun. 69:3703-3712.
23. Mernaugh, G. R., S. F. Dallo, S. C. Holt, and J. B. Baseman. 1993. Properties of adhering and nonadhering populations of Mycoplasma genitalium. Clin. Infect. Dis. 17(Suppl. 1):S69-S78.
24. Miyata, M., and S. Seto. 1999. Cell reproduction cycle of mycoplasma. Biochimie 81:873-878.
25. Morrison-Plummer, J., D. H. Jones, K. Daly, J. G. Tully, D. Taylor-Robinson, and J. B. Baseman. 1987. Molecular characterization of Mycoplasma genitalium species-specific and cross-reactive determinants: identification of an immunodominant protein of M. genitalium. Isr. J. Med. Sci. 23:453-457.
26. Morrison-Plummer, J., A. Lazzell, and J. B. Baseman. 1987. Shared epitopes between Mycoplasma pneumoniae major adhesin protein P1 and a 140-kilodalton protein of Mycoplasma genitalium. Infect. Immun. 55:49-56.
27. Musatovova, O., S. Dhandayuthapani, and J. B. Baseman. 2003. Transcriptional starts for cytadherence-related operons of Mycoplasma genitalium. FEMS Microbiol. Lett. 229:73-81.
28. Musatovova, O., C. Herrera, and J. B. Baseman. 2006. Proximal region of the gene encoding cytadherence-related protein permits molecular typing of Mycoplasma genitalium clinical strains by PCR-restriction fragment length polymorphism. J. Clin. Microbiol. 44:598-603.
29. Peterson, S. N., C. C. Bailey, J. S. Jensen, M. B. Borre, E. S. King, K. F. Bott, and C. A. Hutchison III. 1995. Characterization of repetitive DNA in the Mycoplasma genitalium genome: possible role in the generation of antigenic variation. Proc. Natl. Acad. Sci. USA 92:11829-11833.
30. Pich, O. Q., R. Burgos, M. Ferrer-Navarro, E. Querol, and J. Pinol. 2006. Mycoplasma genitalium mg200 and mg386 genes are involved in gliding motility but not in cytadherence. Mol. Microbiol. 60:1509-1519.
31. Pich, O. Q., R. Burgos, R. Planell, E. Querol, and J. Pinol. 2006. Comparative analysis of antibiotic resistance gene markers in Mycoplasma genitalium: application to studies of the minimal gene complement. Microbiology 152:519-527.
32. Pollack, J. D., M. V. Williams, and R. N. McElhaney. 1997. The comparative metabolism of the mollicutes (mycoplasmas): the utility for taxonomic classification and the relationship of putative gene annotation and phytogeny to enzymatic function in the smallest free-living cells. Crit. Rev. Microbiol. 23:269-354.
33. Proft, T., and R. Herrmann. 1994. Identification and characterization of hitherto unknown Mycoplasma pneumoniae proteins. Mol. Microbiol. 13: 337-348.
34. Proft, T., H. Hilbert, H. Plagens, and R. Herrmann. 1996. The P200 protein of Mycoplasma pneumoniae shows common features with the cytadherence-associated proteins HMW1 and HMW3. Gene 171:79-82.
35. Reddy, S. P., W. G. Rasmussen, and J. B. Baseman. 1996. Isolation and characterization of transposon Tn4001-generated, cytadherence-deficient transformants of Mycoplasma pneumoniae and Mycoplasma genitalium. FEMS Immunol. Med. Microbiol. 15:199-211.
36. Reddy, S. P., W. G. Rasmussen, and J. B. Baseman. 1995. Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium. J. Bacteriol. 177:5943-5951.
37. Regula, J. T., G. Boguth, A. Gorg, J. Hegermann, F. Mayer, R. Frank, and R. Herrmann. 2001. Defining the mycoplasma 'cytoskeleton': the protein composition of the Triton X-100 insoluble fraction of the bacterium Mycoplasma pneumoniae determined by 2-D gel electrophoresis and mass spectrometry. Microbiology 147:1045-1057.
38. Romero-Arroyo, C. E., J. Jordan, S. J. Peacock, M. J. Willby, M. A. Farmer, and D. C. Krause. 1999. Mycoplasma pneumoniae protein P30 is required for cytadherence and associated with proper cell development. J. Bacteriol. 181:1079-1087.
39. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
40. Seto, S., T. Kenri, T. Tomiyama, and M. Miyata. 2005. Involvement of P1 adhesin in gliding motility of Mycoplasma pneumoniae as revealed by the inhibitory effects of antibody under optimized gliding conditions. J. Bacteriol. 187:1875-1877.
41. Su, C. J., A. Chavoya, and J. B. Baseman. 1989. Spontaneous mutation results in loss of the cytadhesin (P1) of Mycoplasma pneumoniae. Infect. Immun. 57:3237-3239.
42. Svenstrup, H. F., J. S. Jensen, K. Gevaert, S. Birkelund, and G. Christiansen. 2006. Identification and characterization of immunogenic proteins of mycoplasma genitalium. Clin. Vaccine Immunol. 13:913-922.
43. Taylor-Robinson, D. 2002. Mycoplasma genitalium-an up-date. Int. J. STD AIDS 13:145-151.
44. Taylor-Robinson, D., C. B. Gilroy, S. Horowitz, and J. Horowitz. 1994. Mycoplasma genitalium in the joints of two patients with arthritis. Eur. J. Clin. Microbiol. Infect. Dis. 13:1066-1069.
45. Tully, J. G., D. L. Rose, J. B. Baseman, S. F. Dallo, A. L. Lazzell, and C. P. Davis. 1995. Mycoplasma pneumoniae and Mycoplasma genitalium mixture in synovial fluid isolate. J. Clin. Microbiol. 33:1851-1855.
46. van der Woude, M. W., and A. J. Baumler. 2004. Phase and antigenic variation in bacteria. Clin. Microbiol. Rev. 17:581-611.
47. Waldo, R. H., III, and D. C. Krause. 2006. Synthesis, stability, and function of cytadhesin P1 and accessory protein B/C complex of Mycoplasma pneumoniae. J. Bacteriol. 188:569-575.
48. Weiner, J., III, C. U. Zimmerman, H. W. Gohlmann, and R. Herrmann. 2003. Transcription profiles of the bacterium Mycoplasma pneumoniae grown at different temperatures. Nucleic Acids Res. 31:6306-6320.
49. Winner, F., I. Markova, P. Much, A. Lugmair, K. Siebert-Gulle, G. Vogl, R. Rosengarten, and C. Citti. 2003. Phenotypic switching in Mycoplasma gallisepticum hemadsorption is governed by a high-frequency, reversible point mutation. Infect. Immun. 71:1265-1273.