High-resolution quantification of focal adhesion spatiotemporal dynamics in living cells

PLoS ONE

Volumn 6, Issue 7, 2011, Pages

  Berginski, Mathew E ^a   Vitriol, Eric A ^b   Hahn, Klaus M ^b,d   Gomez, Shawn M ^a,c,d  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c Brooks Computer Science Building   (United States)

^d UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PAXILLIN; SERINE; STRESS ACTIVATED PROTEIN KINASE; FOCAL ADHESION KINASE 1; PTK2 PROTEIN, MOUSE;

ARTICLE; CELL KINETICS; CELL SHAPE; CELL SIZE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; FIBROBLAST; FLUORESCENCE MICROSCOPY; FOCAL ADHESION; MOLECULAR DYNAMICS; POINT MUTATION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; ANIMAL; CELL STRAIN 3T3; GENETICS; KINETICS; METABOLISM; MOUSE;

ANIMALS; FOCAL ADHESION KINASE 1; FOCAL ADHESIONS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; KINETICS; MICE; MICROSCOPY, FLUORESCENCE; NIH 3T3 CELLS; PAXILLIN; POINT MUTATION;

EID: 79960334368     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0022025     Document Type: Article

References (30)

1. Geiger B, Spatz JP, Bershadsky AD, (2009) Environmental sensing through focal adhesions. Nat Rev Mol Cell Biol 10: 21-33.
2. Lauffenburger DA, Horwitz AF, (1996) Cell migration: a physically integrated molecular process. Cell 84.
3. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, et al. (2003) Cell Migration: Integrating Signals from Front to Back. Science 302.
4. Larsen M, Tremblay ML, Yamada KM, (2003) Phosphatases in cell-matrix adhesion and migration. Nat Rev Mol Cell Biol 4: 700-711.
5. Webb DJ, Parsons JT, Horwitz AF, (2002) Adhesion assembly, disassembly and turnover in migrating cells- over and over and over again. Nat Cell Biol 4.
6. Ballestrem C, Hinz B, Imhof BA, Wehrle-Haller B, (2001) Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior. J Cell Biol 155: 1319-1332.
7. Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, et al. (2004) FAK--Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nature Cell Biology 6.
8. Nayal A, Webb DJ, Brown CM, Schaefer EM, Vicente-Manzanares M, et al. (2006) Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics. The Journal of Cell Biology 173.
9. Bryce NS, Clark ES, Leysath JL, Currie JD, Webb DJ, et al. (2005) Cortactin promotes cell motility by enhancing lamellipodial persistence. Curr Biol 15: 1276-1285.
10. Mattheyses AL, Simon SM, Rappoport JZ, (2010) Imaging with total internal reflection fluorescence microscopy for the cell biologist. Journal of Cell Science 123: 3621-3628.
11. Owen KA, Pixley FJ, Thomas KS, Vicente-Manzanares M, Ray BJ, et al. (2007) Regulation of lamellipodial persistence, adhesion turnover, and motility in macrophages focal adhesion kinase. J Cell Biol.
12. Kobayashi T, Hino S-i, Oue N, Asahara T, Zollo M, et al. (2006) Glycogen Synthase Kinase 3 and h-prune Regulate Cell Migration Modulating Focal Adhesions. Mol Cell Biol 26.
13. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, et al. (2004) Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat Cell Biol 6.
14. Paran Y, Ilan M, Kashman Y, Goldstein S, Liron Y, et al. (2007) High-throughput screening of cellular features using high-resolution light-microscopy; Application for profiling drug effects on cell adhesion. Journal of Structural Biology 158.
15. Zamir E, Geiger B, Kam Z, (2008) Quantitative Multicolor Compositional Imaging Resolves Molecular Domains in Cell-Matrix Adhesions. PLoS ONE 3: e1901.
16. Winograd-Katz SE, Itzkovitz S, Kam Z, Geiger B, (2009) Multiparametric analysis of focal adhesion formation RNAi-mediated gene knockdown. J Cell Biol 186.
17. Brown MC, Turner CE, (2004) Paxillin: Adapting to Change. Physiological Reviews 84.
18. Zaidel-Bar R, Ballestrem C, Kam Z, Geiger B, (2003) Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells. Journal of Cell Science 116.
19. Zaidel-Bar R, Cohen M, Addadi L, Geiger B, (2004) Hierarchical assembly of cell-matrix adhesion complexes. Biochem Soc Trans 32.
20. Huang C, Rajfur Z, Borchers C, Schaller MD, Jacobson K, (2003) JNK phosphorylates paxillin and regulates cell migration. Nature 424.
21. Kimura K, Teranishi S, Yamauchi J, Nishida T, (2008) Role of JNK-Dependent Serine Phosphorylation of Paxillin in Migration of Corneal Epithelial Cells during Wound Closure. Investigative Ophthalmology Visual Science 49.
22. Huang Z, Yan D-P, Ge B-X, (2008) JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin. Cellular Signalling 20.
23. Vallotton P, Gupton SL, Waterman-Storer CM, Danuser G, (2004) Simultaneous mapping of filamentous actin flow and turnover in migrating cells quantitative fluorescent speckle microscopy. PNAS 101.
24. Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, et al. (2011) A role for actin arcs in the leading-edge advance of migrating cells. Nat Cell Biol 13: 371-382.
25. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, et al. (2009) Coordination of Rho GTPase activities during cell protrusion. Nature 461: 99-103.
26. Huang C, Borchers CH, Schaller MD, Jacobson K, (2004) Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells. The Journal of Cell Biology 164: 593-602.
27. Rajfur Z, Roy P, Otey C, Romer L, Jacobson K, (2002) Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins. Nat Cell Biol 4: 286-293.
28. Zamir E, Katz BZ, Aota S, Yamada KM, Geiger B, et al. (1999) Molecular diversity of cell-matrix adhesions. J Cell Sci 112.
29. Ji L, Danuser G, (2005) Tracking quasi-stationary flow of weak fluorescent signals adaptive multi-frame correlation. Journal of Microscopy 220.
30. Machacek M, Danuser G, (2006) Morphodynamic Profiling of Protrusion Phenotypes. Biophys J 90.