P16 INK4a immunocytochemistry on cell blocks as an adjunct to cervical cytology: Potential reflex testing on specially prepared cell blocks from residual liquid-based cytology specimens

CytoJournal

Volumn 8, Issue , 2011, Pages

  Shidham, Vinod B ^a   Mehrotra, Ravi ^b   Varsegi, George ^c   D'Amore, Krista ^c   Hunt, Bryan ^a   Narayan, Raj ^c  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ALLAHABAD   (India)

^c MEDICAL COLLEGE OF WISCONSIN   (United States)

Author keywords

Cervical cancer; cervical cytology; immunohistochemistry; p16; Pap test; reflex testing; screening; squamous intraepithelial neoplasia

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR 2A;

ADULT; AGED; ARTICLE; ATYPICAL SQUAMOUS CELL; ATYPICAL SQUAMOUS CELL OF UNDETERMINED SIGNIFICANCE; CONTROLLED STUDY; CYTOPLASM; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; HIGH GRADE SQUAMOUS INTRAEPITHELIAL LESION; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LIQUID BASED CYTOLOGY; LOW GRADE SQUAMOUS INTRAEPITHELIAL LESION; SENSITIVITY AND SPECIFICITY; SQUAMOUS CELL; UTERINE CERVIX BIOPSY; UTERINE CERVIX CARCINOMA; UTERINE CERVIX CARCINOMA IN SITU; UTERINE CERVIX CYTOLOGY; WART VIRUS;

EID: 79960289546     PISSN: 09745963     EISSN: 17426413     Source Type: Journal    
DOI: 10.4103/1742-6413.76379     Document Type: Article

References (38)

1. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA Cancer J Clin 2007;57:43-66.
2. National Cancer Institute. Previous Version: SEER Cancer Statistics Review, 1975-2005 Available from: http://www.seer.cancer.gov/csr/1975-2006/ results-single/sect-05-table.04.pdf [Last accessed on 2010 Mar 14].
3. Centers for Disease Control and Prevention. Cervical Cancer Trends. Available from: http://www.cdc.gov/cancer/cervical/statistics/trends.htm [Last accessed On 2010 Mar 14].
4. Park SY, Bae DS, Nam JH, Park CT, Cho CH, Lee JM, et al. Quality of life and sexual problems in disease-free survivors of cervical cancer compared with the general population. Cancer 2007;110:2716-25.
5. Chivukula M, Komorowski R, Shidham V. Ancillary application of p16 INK4a immunoexpression for objective grading of dysplasia in cervical biopsies. Mod Pathol 2004;17:1a-388.
6. Chivukula M, Komorowski R, Shidham V. Application of p16 INK4A to evaluate the distribution pattern of mitotic figures and apoptotic bodies for grading cervical dysplasia. Mod Pathol 2004;17:1a-388.
7. Behmaram B, Kotov P, Basir Z, Cafaro A, Novoa-Takara L, Shidham VB. Comparison of inter and intra-observer reproducibility of evaluating cervical dysplasia in HandE stained and p16 immunostained sections. Mod Pathol 2006;19:1a-359. Available from: http://www.nature.com/modpathol/journal/v19/n1s/ pdf/3800847a.pdf [Last accessed on 2010 Jun 12].
8. Wentzensen N, Bergeron C, Cas F, Vinokurova S, von Knebel Doeberitz M. Evaluation of a nuclear score for p16INK4a-stained cervical squamous cells in liquid-based cytology samples. Cancer 2007;111:58-66.
9. Wentzensen N, Bergeron C, Cas F, Eschenbach D, Vinokurova S, von Knebel Doeberitz M. Evaluation of a nuclear score for p16 INK4a-stained cervical squamous cells in liquid-based cytology samples. Cancer 2005;105:461-7. (Pubitemid 43067004)
10. Tsoumpou I, Arbyn M, Kyrgiou M, Wentzensen N, Koliopoulos G, Martin-Hirsch P, et al. 2009 p16(INK4a) immunostaining in cytological and histological specimens from the uterine cervix: A systematic review and meta-analysis. Cancer Treat Rev 2009;35:210-20.
11. Varsegi GM, Shidham V. Cell block preparation from cytology specimen with predominance of individually scattered cells. J Vis Exp 2009;29.doi: 10.3791/1316. PMID: 19623160 Video article is available FREE on web as open access from: http://www.jove.com/index/Details.stp?ID=1316. [Last accessed on 2010 Jun 12].
12. Ungureanu C, Socolov D, Anton G, Mihailovici MS, Teleman S. Immunocytochemical expression of p16 INK4a and HPV L1 capsid proteins as predictive markers of the cervical lesions progression risk. Rom J Morphol Embryol 2010;51:497-503.
13. Chivukula M, Austin RM, Duwe A, Friedman T, Masko J, Mauser N, et al. Dual-Stain for P16 and Ki67 in the Interpretation of Abnormal PAP Cytology Results: A Prospective Study. Mod Pathol 2010;23:85a-109. doi:10.1038/modpathol. 2010.10 Abstract #395. Available from: http://www.nature.com/modpathol/journal/ v23/n1s/pdf/modpathol201010a.pdf [Last accessed on 2010 Jun 12].
14. Kotov P, Parameswaran L, Parisi JA, Chivkula M, Cafaro A, Fuentes M, et al. p16INK4a Immunostaing of Liquid Based Cervical Cytology Smears with SurePath-Comparison of Two Antibodies. Mod Pathol 2005;18:1a-359. Available from: http://www.nature.com/modpathol/journal/v18/n1s/pdf/3800908a.pdf [Last accessed on 2010 Jun 12].
15. Kotov P, Parameswaran L, Parisi JA, Chivkula M, Cafaro A, Fuentes M, et al. Application of p16INK4A Immunostaing for Definitive Interpretation of ASC-H in Liquid Based Cervical Cytology Smears with SurePath. Mod Pathol 2005;18:1a-359. Available from: http://www.nature.com/modpathol/journal/v18/n1s/ pdf/3800908a.pdf [Last accessed on 2010 Jun 12].
16. Shidham V, D'Amore K, Varsegi G. Objective and definitive subcategorization of LSIL with p16INK Immunocytochemistry on Cell block Sections of Cervical Cytology Specimens. Cancer Cytopathol 2009;117:349-450.
17. Liu H, Shi J, Wilkerson M, Huang Y, Meschter S, Dupree W, et al. Immunohistochemical detection of p16 INK4a in liquid-based cytology specimens on cell block sections. Cancer 2007;111:74-82. (Pubitemid 46648405)
18. Akpolat I, Smith DA, Ramzy I, Chirala M, Mody DR. The utility of p16 INK4a and Ki-67 staining on cell blocks prepared from residual thin-layer cervicovaginal material. Cancer 2004;102:142-9. (Pubitemid 38780598)
19. Meyer JL, Hanlon DW, Andersen BT, Rasmussen OF, Bisgaard K. Evaluation of p16 INK4a expression in ThinPrep cervical specimens with the CINtec p16 INK4a assay. Cancer 2007;111:83-92.
20. Chivukula M, Shidham V. ASC-H in Pap test-definitive categorization of cytomorphological spectrum. Cytojournal 2006;3:14. Free full text is Available from: http://www.cytojournal.com/content/3/1/14 PDF Available from; http://www.cytojournal.com/content/pdf/1742-6413-3-14.pdf. [Last accessed on 2010 Jun 12].
21. Kalof AN, Cooper K. p16 INK4a immunoexpression: Surrogate marker of high-risk HPV and high-grade cervica intraepithelial neoplasia. Adv Anat Pathol 2006;13:190-4. (Pubitemid 44341655)
22. Tringler B, Gup CJ, Singh M, Groshong S, Shroyer AL, Heinz DE, et al. Evaluation of p16 INK4a and pRb expression in cervical squamous and glandular neoplasia. Hum Pathol 2004;35:689-96. (Pubitemid 38881079)
23. Klaes R, Woerner SM, Ridder R, Wentzensen N, Duerst M, Schneider A, et al. Detection of high risk cervical intraepithelial neoplasia and cervical cancer byamplification of transcripts derived from integrated papillomavirus oncogenes. Cancer Res 1999;59:6132-6. (Pubitemid 30035633)
24. Klaes R, Benner A, Friedrich T, Ridder R, Herrington S, Jenkins D, et al. p16 INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 2002;26:1389-99. (Pubitemid 35239415)
25. Leversha MA, Fielding P, Watson S, Gosney JR, Field JK. Expression of p53, pRB, and p16 in lung tumours: A validation study on tissue microarrays. J Pathol 2003;200:610-9. (Pubitemid 36987310)
26. Mkitie AA, MacMillan C, Ho J, Shi W, Lee A, O'Sullivan B, et al. Loss of p16 expression has prognostic significance in human nasopharyngeal carcinoma. Clin Cancer Res 2003;9:2177-84. (Pubitemid 36687641)
27. Yu L, Wang L, Zhong J, Chen S. Diagnostic value of p16INK4A, Ki-67, and human papillomavirus l1 capsid protein immunochemical staining on cell blocks from residual liquid-based gynecologic cytology specimens. Cancer Cytopathol 2010;118:47-55.
28. Varsegi G, D'Amore K, Shidham V. p16ink4a immunocytochemistry as an adjunct to cervical cytology-potential reflex testing on specially prepared cellblocks from residual liquid based cytology (lbc) specimens. Mod Pathol 2009;22:p97a. Available from; http://www.nature.com/modpathol/journal/v22/n1s/ pdf/modpathol2008212a.pdf [Last accessed on 2010 Jun 12].
29. Stoler MH, Schiffman M. Interobserver Reproducibility of Cervical Cytologic and Histologic Interpretations: Realistic Estimates From the ASCUS-LSIL Triage Study. JAMA 2001;285:500-5.
30. Bose S, Evans H, Lantzy L, Scharre K, Youssef E. p16(INK4A) is a surrogate biomarker for a subset of human papilloma virus-associated dysplasias of the uterine cervix as determined on the Pap smear. Diagn Cytopathol 2005;32:21-4. (Pubitemid 40024069)
31. del Pino M, Garcia S, Fust V, Alonso I, Fust P, Torn A, et al. Value of p16(INK4a) as a marker of progression/regression in cervical intraepithelial neoplasia grade 1. Am J Obstet Gynecol 2009;201:488.e1-7.
32. Shidham VB, Kumar N, Narayan R, Brotzman GL. Should LSIL with ASC-H (LSIL-H) in cervical smears be an independent category? A study on SurePath TM specimens with review of literature. Cytojournal 2007,4:7. Free full text is Available from: http://www.cytojournal.com/content/4/1/7 PDF Available from: http://www.cytojournal.com/content/pdf/1742-6413-4-7.pdf.[Last accessed on 2010 Jun 12].
33. American Society for Colposcopy and Cervical Pathology guidelines Available from: http://www.asccp.org/pdfs/consensus/algorithms-cyto-07.pdf (ASCCP-Available from: http://www.asccp.org/hpv.shtml) [Last accessed on 2010 Mar 02].
34. Luesley DM, McCrum A, Terry PB, Wade-Evans T, Nicholson HO, Mylotte MJ, et al. Complications of cone biopsy related to the dimensions of the cone and the influence of prior colposcopic assessment. Br J Obstet Gynaecol 1985;92:158-64. (Pubitemid 15162721)
35. Wentzensen N, Bergeron C, Cas F, Vinokurova S, von Knebel Doeberitz M. Triage of women with ASCUS and LSIL cytology. Cancer 2006;111:58-66.
36. Shidham VB, Varsegi G, D'Amore K. Two-color immunocytochemistry for evaluation of effusion fluids for metastatic adenocarcinoma. Cytojournal 2010;7:1. Available from: http://alturl.com/x4rg [Last accessed on 2010 Jun 12].
37. Halloush RA, Akpolat I, Jim Zhai Q, Schwartz MR, Mody DR. Comparison of ProEx C with p16 INK4a and Ki-67 immunohistochemical staining of cell blocks prepared from residual liquid-based cervicovaginal material: A pilot study. Cancer 2008;114:474-80.
38. Shidham VB, Atkinson BF. Immunocytochemistry of effusion fluids: Introduction to the SCIP approach. In: Shidham VB, Atkinson BF, editors. 'Cytopathologic Diagnosis of Serous Fluids' Ch 5. 1 st ed. Elsevier: W. B. Saunders Company; 2007. p. 55-78.