Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells

Biotechnology and Bioengineering

Volumn 107, Issue 4, 2010, Pages 683-695

  Storm, Michael P ^a,b   Orchard, Craig B ^a,b   Bone, Heather K ^a   Chaudhuri, Julian B ^b   Welham, Melanie J ^a  

^a University of Bath   (United Kingdom)

^b UNIVERSITY OF BATH   (United Kingdom)

Author keywords

3D agitated suspension culture; Bioreactors; Embryonic stem cells; Microcarriers; Pluripotency

Indexed keywords

3D CULTURE; AGITATED SYSTEMS; AGITATION CONDITIONS; CELL NUMBER; DRUG DISCOVERY; EMBRYONIC STEM CELLS; LARGE-SCALE PRODUCTION; MAMMALIAN CELLS; MICROCARRIERS; MOUSE EMBRYONIC STEM CELLS; OPTIMIZED CONDITIONS; PLURIPOTENCY; PLURIPOTENT; PROOF OF CONCEPT; STIRRED BIOREACTORS; SUSPENSION CULTURES; THERAPEUTIC AGENTS; THREE-DIMENSIONAL (3D); THREE-DIMENSIONAL CULTURE SYSTEM;

ANIMAL CELL CULTURE; BIOCONVERSION; BIOREACTORS; CELL CULTURE; CELL GROWTH; EXPANSION; MAMMALS; OPTIMIZATION; STEM CELLS;

THREE DIMENSIONAL;

OCTAMER TRANSCRIPTION FACTOR 4; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRANSCRIPTION FACTOR NANOG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOREACTOR; CELL ADHESION; CELL AGGREGATION; CELL COUNT; CELL DIFFERENTIATION; CELL EXPANSION; CELL LINEAGE; CELL PROLIFERATION; EMBRYONIC STEM CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MAMMAL CELL; MICROCARRIER CULTURE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; EMBRYONIC STEM CELLS; HUMANS; MICE; MICROSPHERES;

MAMMALIA; MURINAE;

EID: 78149330177     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22850     Document Type: Article

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