Expression of a recombinant chimeric protein of hepatitis A virus VP1-Fc using a replicating vector based on Beet curly top virus in tobacco leaves and its immunogenicity in mice

Plant Cell Reports

Volumn 30, Issue 8, 2011, Pages 1513-1521

  Chung, Ho Yong ^a   Lee, Hyun Ho ^a   Kim, Kyung Il ^a   Chung, Ha Young ^a   Hwang Bo, Jeon ^a   Park, Jong Hwa ^a   Sunter, Garry ^b   Kim, Jong Bum ^c   Shon, Dong Hwa ^d   Kim, Wonyong ^e   Chung, In Sik ^a  

^a Kyung Hee University   (South Korea)

^b UNIVERSITY OF TEXAS AT SAN ANTONIO   (United States)

^c National Institute of Animal Science   (South Korea)

^d Korea Food Research Institute   (South Korea)

^e Chung Ang University College of Medicine   (South Korea)

Author keywords

Beet curly top virus; Expression; Hepatitis A virus; Immunogenicity; Nicotiana benthamiana; Recombinant VP1

Indexed keywords

GAMMA INTERFERON; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; INTERLEUKIN 4; VIRUS ANTIBODY; VIRUS PROTEIN; VP1 PROTEIN, HEPATITIS A VIRUS;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; BIOSYNTHESIS; BLOOD; CELL CULTURE; CROSS REACTION; FEMALE; GENE VECTOR; GENETICS; HEPATITIS A VIRUS; IMMUNIZATION; IMMUNOLOGY; MOUSE; PLANT VIRUS; TOBACCO; TRANSGENIC PLANT; VIROLOGY;

ANIMALS; ANTIBODIES, VIRAL; CELLS, CULTURED; CROSS REACTIONS; FEMALE; GENETIC VECTORS; HEPATITIS A VIRUS; IMMUNIZATION; IMMUNOGLOBULIN FC FRAGMENTS; INTERFERON-GAMMA; INTERLEUKIN-4; MICE; MICE, INBRED BALB C; PLANT VIRUSES; PLANTS, GENETICALLY MODIFIED; RECOMBINANT FUSION PROTEINS; TOBACCO; VIRAL STRUCTURAL PROTEINS;

AGROBACTERIUM; BEET CURLY TOP VIRUS; BETA VULGARIS SUBSP. VULGARIS; HEPATITIS A VIRUS; HUMAN HEPATITIS A VIRUS; MUS; NICOTIANA BENTHAMIANA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; RHIZOBIUM;

EID: 79960243876     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-011-1062-6     Document Type: Article

References (21)

1. Ainsworth C, Benyon J, Buchanan-Wollaston V (1996) Techniques in plant molecular biology. Wye College Publications, London.
2. Arnon R (1991) Synthetic peptides as the basis for vaccine design. Mol Immunol 28: 209-215.
3. Bertoletti A, Gehring AJ (2006) The immune response during hepatitis B virus infection. J Gen Virol 87: 1439-1449.
4. Emini EA, Hughes JV, Perlow DS, Boger J (1985) Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J Virol 55: 836-839.
5. Fisher R, Stoger E, Schillberg S, Christou P, Twyman RM (2004) Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7: 152-158.
6. Hamon SA, Johnston JM, Ziegelhoffer T, Richards OC, Summers DF, Ehrenfeld E (1988) Expression of hepatitis A virus capsid sequences in insect cells. Virus Res 10: 273-280.
7. Hughes JV, Stanton LW (1985) Isolation and immunizations with hepatitis A viral structural proteins: induction of antiprotein, antiviral, and neutralizing responses. J Virol 55: 395-401.
8. Hughes JV, Stanton LW, Tomassini JE, Long WJ, Scolnick EM (1984) Neutralizing monoclonal antibodies to hepatitis A virus: partial localization of a neutralizing antigenic site. J Virol 52: 465-473.
9. Kim KI, Sunter G, Bisaro DM, Chung IS (2007) Improved expression of recombinant GFP using a replicating vector based on Beet curly top virus in leaf-disks and infiltrated Nicotiana benthamiana leaves. Plant Mol Biol 64: 103-112.
10. Lee JM, Hwang-Bo J, Sohn BH, Chung IS (2007) Functional expression of recombinant canstatin in stably transformed Drosophila melanogaster S2 cells. Protein Expr Purif 52: 258-264.
11. Lee JM, Lee HH, Hwang-Bo J, Shon DH, Kim W, Chung IS (2009) Expression and immunogenicity of recombinant polypeptide VP1 of human hepatitis A virus in stably transformed fruitfly (Drosophila melanogaster) Schneider 2 cells. Biotechnol Appl Biochem 53: 101-109.
12. Lo KM, Sudo Y, Chen J, Li Y, Lan Y, Kong SM, Chen L, An Q, Gillies SD (1998) High level expression and secretion of Fc-X fusion proteins in mammalian cells. Prot Eng 11: 495-500.
13. Obregon P, Chargelegue D, Drake PM, Prada A, Nuttall J, Frigerio L, Ma JK (2006) HIV-1 p24-immunoglobin fusion molecule: a new strategy for plant-based protein production. Plant Biotechnol J 4: 195-207.
14. Ostermayr R, Helm K, Gauss-muller V, Winnacker EL, Deinhardt F (1987) Expression of hepatitis A virus cDNA in E. coli: antigenic VP1 recombinant proteins. J Virol 61: 3645-3647.
15. Regnard GL, Halley-Stott RP, Tanzer FL, Hitzeroth II, Rybicki EP (2010) High level protein expression in plants though the use of a novel autonomously replicating geminivirus shuttle vector. Plant Biotechnol J 8: 38-46.
16. Rengarajan J, Szabo SJ, Glimcher LH (2000) Transcriptional regulation of Th1/Th2 polarization. Immunol Today 21: 479-483.
17. Seder RA, Paul WE (1994) Acquisition of lymphokine-producing phenotype by CD4+ T cells. Annu Rev Immunol 12: 635-673.
18. Seok YJ, Kim KI, Yoo KH, Hwang-Bo J, Lee HH, Shon DH, Ko KS, Kang HS, Lee KJ, Oh DB, Joung YH, Chung IS (2010) Expression and immunogenicity of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and an Fc antibody fragment in stably transformed Drosophila melanogaster S2 cells. Appl Biochem Biotechnol 162: 1435-1445.
19. Spitsin S, Andrianov V, Pogrebnyak N, Smirnov Y, Borisjuk N, Portocarrero C, Veguilla V, Koprowski H, Golovkin M (2009) Immunological assessment of plant-derived avian flu H5/HA1 variants. Vaccine 27: 1289-1292.
20. Stapleton JT, Lemon SM (1987) Neutralization escape mutants define a dominant immunogenic neutralization site on hepatitis A virus. J Virol 61: 491-498.
21. Twyman RM, Stoger E, Schillberg S, Christou P, Fisher R (2003) Molecular farming in plants: host systems and expression technology. Trend Biotechnol 21: 570-578.