Functional expression of recombinant canstatin in stably transformed Drosophila melanogaster S2 cells

Protein Expression and Purification

Volumn 52, Issue 2, 2007, Pages 258-264

  Lee, Jong Min ^a   Jeon, Hwang Bo ^a   Sohn, Bong Hee ^b   Chung, In Sik ^a  

^a Kyung Hee University   (South Korea)

^b National Institute of Animal Science   (South Korea)

Author keywords

Drosophila melanogaster S2 cells; Expression; In vitro activity; Purification; Recombinant canstatin

Indexed keywords

CANSTATIN PROTEIN, HUMAN; COLLAGEN TYPE 4; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL LINE; CELL PROLIFERATION; CYTOLOGY; DROSOPHILA MELANOGASTER; ENDOTHELIUM CELL; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PHYSIOLOGY; TIME;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE, TRANSFORMED; CELL PROLIFERATION; CELLS, CULTURED; COLLAGEN TYPE IV; DROSOPHILA MELANOGASTER; ENDOTHELIAL CELLS; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; RECOMBINANT PROTEINS; TIME FACTORS;

DROSOPHILA MELANOGASTER;

EID: 33846461148     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.11.016     Document Type: Article

References (18)

1. Kerbel R.S. A cancer therapy resistant to resistance. Nature 390 (1997) 335-336
2. Hanahan D., and Folkman J. Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86 (1996) 353-364
3. O'Reilly M.S., Boehm T., Shing Y., Fukai N., Vasios G., Lane W.S., Flynn E., Birkhead J.R., Olsen B.R., and Folkman J. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell 88 (1997) 277-285
4. Maeshima Y., Colorado P.C., Torre A., Holthaus K.A., Grunkemeyer J.A., Ericksen M.B., Hopfer H., Xiao Y., Stillman I.E., and Kalluri R. Distinct antitumor properties of a type IV collagen domain derived from basement membrane. J. Biol. Chem. 275 (2000) 21340-21348
5. Kamphaus G.D., Colorado P.C., Panka D.J., Hopfer H., Ramchandran R., Torre A., Maeshima Y., Mier J.W., Sukhatme V.P., and Kalluri R. Canstatin, a novel matrix-derived inhibitor of angiogenesis and tumor growth. J. Biol. Chem. 275 (2000) 1209-1215
6. Darland D.C., and D'Amore P.A. Blood vessel maturation: vascular development comes of age. J. Clin. Invest. 103 (1999) 157-158
7. Maeshima Y., Manfredi M., Reimer C., Holthaus K.A., Hopfer H., Chandamuri B.R., Kharbanda S., and Kalluri R. Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin. J. Biol. Chem. 276 (2001) 15240-15248
8. Panka D.J., and Mier J.W. Canstatin inhibits Akt activation and induces Fas-dependent apoptosis in endothelial cells. J. Biol. Chem. 278 (2003) 37632-37636
9. 5 integrins. Cancer Res. 65 (2005) 4353-4361
10. Pasternak J.J. DNA sequence organization and gene copy number determination. In: Glick B.R., and Thompson J.E. (Eds). Methods in Plant Molecular Biology and Biotechnology (1993), CRC Press, Inc., Ann Arbor 29-36
11. Laemmli U.K. Cleavage of structural proteins during the assembly of head of bacteriophage. Nature 227 (1970) 680-685
12. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbour Laboratory Press, New York
13. Shevchenko A., Wilm M., Vorm O., and Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68 (1996) 850-858
14. Choi B.K., Cho Y.M., Bae S.H., Zoubaulis C.C., and Paik Y.K. Single-step perfusion chromatography with a throughput potential for enhanced peptide detection by matrix-assisted laser desorption/ionization-mass spectrometry. Proteomics 3 (2003) 1955-1961
15. Finney D.J. Probit analysis. third ed. (1971), Cambridge University Press, Cambridge
16. Johansen H., van der Straten A., Sweet R., Otto E., Maroni G., and Rosenberg M. Regulated expression at high copy number allows production of a growth-inhibitory oncogene product in Drosophila Schneider cells. Genes Dev. 3 (1989) 882-889
17. Angelichio M.L., Beck J.A., Johansen H., and Ivey-Hoyle M. Comparison of several promoters and polyadenylation signals for use in heterologous gene expression in cultured Drosophila cells. Nucleic Acids Res. 19 (1991) 5037-5043
18. Ivey-Hoyle M., Culp J.S., Chaikin M.A., Hellmig B.D., Matthews T.J., Sweet R.W., and Rosenberg M. Envelope glycoproteins from biologically diverse isolates of immunodeficiency viruses have widely different affinities for CD4. Proc. Natl. Acad. Sci. USA 88 (1991) 512-516