Large-scale plasmid preparation for transient gene expression

Biotechnology Letters

Volumn 33, Issue 8, 2011, Pages 1559-1564

  Cheng, Lu ^a   Sun, Xiangming ^b   Yi, Xiaoping ^a   Zhang, Yuanxing ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b Shanghai Kexin Biotech Co Ltd   (China)

Author keywords

CHO cells; Fed batch; Plasmid DNA; RNase; Transient transfection; Ultrafiltration

Indexed keywords

CHO CELL; FED BATCHES; PLASMID DNA; RNASE; TRANSIENT TRANSFECTION;

ANIMAL CELL CULTURE; BATCH CELL CULTURE; BIOMOLECULES; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GLUCOSE; MAMMALS; ULTRAFILTRATION;

DNA;

GLUCOSE; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; RIBONUCLEASE;

ANIMAL; ARTICLE; BIOREACTOR; BIOSYNTHESIS; CHEMISTRY; CHO CELL; CRICETULUS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENETICS; HAMSTER; ISOLATION AND PURIFICATION; METABOLISM; PLASMID; PROTEIN ENGINEERING; ULTRAFILTRATION;

ANIMALS; BIOREACTORS; CHO CELLS; CRICETINAE; CRICETULUS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GLUCOSE; GREEN FLUORESCENT PROTEINS; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RIBONUCLEASES; ULTRAFILTRATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 79960164604     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0612-x     Document Type: Article

References (13)

1. Balan S, Murphy J, Galaev I, Kumar A, Fox GE, Mattiasson B, Willson RC (2003) Metal chelate affinity precipitation of RNA and purification of plasmid DNA. Biotechnol Lett 25: 1111-1116.
2. Baldi L, Muller N, Picasso S, Jacquet R, Girard P, Thanh HP, Derow E, Wurm FM (2005) Transient gene expression in suspension HEK-293 cells: application to large-scale protein production. Biotechnol Prog 21: 148-153.
3. Ferreira GNM, Cabral J, Prazeres DMF (1999) Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications. Biotechnol Prog 15: 725-731.
4. Girard P, Derouazi M, Baumgartner G, Bourgeois M, Jordan M, Jacko B, Wurm FW (2002) 100-liter transient transfection. Cytotechnology 38: 15-21.
5. Kahn DW, Butler MD, Cohen DL, Gordon M, Kahn JW, Winkler ME (2000) Purification of plasmid DNA by tangential flow filtration. Biotechnol Bioeng 69: 101-106.
6. Kendall D, Booth AJ, Levy MS, Lye GJ (2001) Separation of supercoiled and open-circular plasmid DNA by liquid-liquid counter-current chromatography. Biotechnol Lett 23: 613-619.
7. Lander RJ, Winters MA, Meacle FJ, Buckland BC, Lee AL (2002) Fractional precipitation of plasmid DNA from lysate by CTAB. Biotechnol Bioeng 79: 776-784.
8. Muller N, Derouazi M, van Tilborgh F, Wulhfard S, Hacker DL, Jordan M, Wurm FW (2007) Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems. Biotechnol Lett 29: 703-711.
9. Prazeres DMF, Schluep T, Cooney C (1998) Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography. J Chromatogr A 806: 31-45.
10. Rozkov A, Larsson B, Gillström S, Björnestedt R, Schmidt SR (2008) Large-scale production of endotoxin-free plasmids for transient expression in mammalian cell culture. Biotechnol Bioeng 99: 557-566.
11. Tait AS, Brown CJ, Galbraith DJ, Hines MJ, Hoare M, Birch JR, James DC (2004) Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents. Biotechnol Bioeng 88: 707-721.
12. Wright JL, Jordan M, Wurm FM (2001) Extraction of plasmid DNA using reactor scale alkaline lysis and selective precipitation for scalable transient transfection. Cytotechnology 35: 165-173.
13. Wurm F, Bernard A (1999) Large-scale transient expression in mammalian cells for recombinant protein production. Curr Opin Biotechnol 10: 156-159.