De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis

Biotechnology Letters

Volumn 33, Issue 8, 2011, Pages 1575-1580

  Li, Haojian ^a   Zhang, Guoqiang ^b,c   Deng, Aihua ^b,c   Chen, Ning ^a   Wen, Tingyi ^b  

^a TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Bacillus subtilis; Fermentation; Genetic engineering; Inosine

Indexed keywords

ADENOSINE MONOPHOSPHATE; BACILLUS SUBTILIS; CONVERSION RATIO; DOUBLE MUTANTS; FERMENTATIVE PRODUCTION; INOSINE; METABOLIC FLUX ANALYSIS; MONOPHOSPHATES; SUBTILIS; WILD TYPES; WILD-TYPE STRAIN;

BACTERIOLOGY; BIOCHEMISTRY; BIOSYNTHESIS; GENETIC ENGINEERING; GLUCOSE; METABOLISM;

BACILLI;

GLUCOSE; INOSINE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BIOSYNTHESIS; CELL PROLIFERATION; ENZYMOLOGY; FERMENTATION; GENE INACTIVATION; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; MUTATION;

BACILLUS SUBTILIS; CELL PROLIFERATION; FERMENTATION; GENE KNOCKOUT TECHNIQUES; GENES, BACTERIAL; GENETIC ENGINEERING; GLUCOSE; INDUSTRIAL MICROBIOLOGY; INOSINE; METABOLIC NETWORKS AND PATHWAYS; MUTATION;

BACILLUS SUBTILIS;

EID: 79960163315     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0597-5     Document Type: Article

References (14)

1. Anagnostopoulos C, Spizizen J (1961) Requirements for transformation in Bacillus subtilis. J Bacteriol 81: 741-746.
2. Chen N, Huang J, Feng Z, Yu L, Xu Q, Wen T (2009) Optimization of fermentation conditions for the biosynthesis of l-threonine by Escherichia coli. Appl Biochem Biotechnol 158: 595-604.
3. Duan Y, Chen T, Chen X, Zhao X (2010) Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis. Appl Microbiol Biotechnol 85: 1907-1914.
4. Kunst F, Ogasawara N, Moszer I, Albertini A, Alloni G, Azevedo V, Bertero M, Bessieres P, Bolotin A, Borchert S (1997) The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390: 237-238.
5. Sauer U, Cameron D, Bailey J (1998) Metabolic capacity of Bacillussubtilis for the production of purine nucleosides, riboflavin, and folic acid. Biotechnol Bioeng 59: 227-238.
6. Schuch R, Garibian A, Saxild H, Piggot P, Nygaard P (1999) Nucleosides as a carbon source in Bacillus subtilis: characterization of the drm-pupG operon. Microbiology 145: 2957.
7. Shimaoka M, Kawasaki H, Takenaka Y, Kurahashi O, Matsui H (2005) Effects of edd and pgi disruptions on inosine accumulation in Escherichia coli. Biosci Biotechnol Biochem 69: 1248-1255.
8. Shimaoka M, Takenaka Y, Mihara Y, Kurahashi O, Kawasaki H, Matsui H (2006) Effects of xapA and guaA disruption on inosine accumulation in Escherichia coli. Biosci Biotechnol Biochem 70: 3069-3072.
9. Shimaoka M, Takenaka Y, Kurahashi O, Kawasaki H, Matsui H (2007) Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli. J Biosci Bioeng 103: 255-261.
10. Srivatsan A, Han Y, Peng J, Tehranchi A, Gibbs R, Wang J, Chen R (2008) High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies. PLoS Genet 4: e1000139.
11. Torii T, Izawa K, Jang D, Cho D (2010) Inosine derivatives and production methods therefor. United States Patent 7816513.
12. Wang H, Isaacs F, Carr P, Sun Z, Xu G, Forest C, Church G (2009) Programming cells by multiplex genome engineering and accelerated evolution. Nature 460: 894-898.
13. Wu L, Li Z, Ye Q (2009) Enhanced d-ribose biosynthesis in batch culture of a transketolase-deficient Bacillus subtilis strain by citrate. J Ind Microbiol Biotechnol 36: 1289-1296.
14. Zakataeva NP, Gronskiy SV, Sheremet AS, Kutukova EA, Novikova AE, Livshits VA (2007) A new function for the Bacillus PbuE purine base efflux pump: efflux of purine nucleosides. Res Microbiol 158: 659-665.