Development of an effective method for recovery of viral genomic RNA from environmental silty sediments for quantitative molecular detection

Applied and Environmental Microbiology

Volumn 77, Issue 12, 2011, Pages 3975-3981

  Miura, Takayuki ^a,b   Masago, Yoshifumi ^a   Sano, Daisuke ^b   Omura, Tatsuo ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b HOKKAIDO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DIRECT METHOD; ESTUARINE SEDIMENTS; GENOMIC RNA; GEOMETRIC MEAN; GEOMETRIC STANDARD DEVIATIONS; INDIRECT METHODS; ISO-PROPANOLS; MAGNETIC BEADS; MOLECULAR DETECTION; MOLECULAR METHODS; PATHOGENIC VIRUS; POLIOVIRUS 1; QUANTIFICATION LIMIT; QUANTITATIVE DATA; QUANTITATIVE PCR; REVERSE TRANSCRIPTION; RNA EXTRACTION; RNA VIRUS; SEDIMENT SAMPLES; SILTY SEDIMENTS; VIRAL CAPSIDS; VIRAL PARTICLES; VIRAL RNA; WET WEIGHT;

ANOXIC SEDIMENTS; COMPUTER VIRUSES; GENES; PHENOLS; RECOVERY; RIVERS; RNA; SEDIMENTOLOGY; VIRUSES;

POLYMERASE CHAIN REACTION;

VIRUS RNA;

ALCOHOL; BAY; CONCENTRATION (COMPOSITION); DETECTION METHOD; EDTA; ESTUARINE SEDIMENT; FLUVIAL DEPOSIT; GENE EXPRESSION; GENOMICS; INOCULATION; MOLECULAR ANALYSIS; PATHOGEN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RNA; SEDIMENT ANALYSIS; SILT; VIRUS;

ARTICLE; EVALUATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; JAPAN; METHODOLOGY; RNA VIRUS; SEDIMENT; VIROLOGY; VIRUS GENOME; VIRUS LOAD;

GENOME, VIRAL; GEOLOGIC SEDIMENTS; HUMANS; JAPAN; RNA VIRUSES; RNA, VIRAL; VIRAL LOAD; VIROLOGY;

HONSHU; JAPAN; MIYAGI; TOHOKU;

ENTEROVIRUS; HUMAN POLIOVIRUS 1; MIRIDAE; NOROVIRUS; RNA VIRUSES;

EID: 79960107674     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02692-10     Document Type: Article

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