Use of bexB to detect the capsule locus in Haemophilus influenzae

Journal of Clinical Microbiology

Volumn 49, Issue 7, 2011, Pages 2594-2601

  Davis, Gregg S ^a   Sandstedt, Sara A ^a   Patel, Mayuri ^b   Marrs, Carl F ^a   Gilsdorf, Janet R ^a,b  

^a UNIVERSITY OF MICHIGAN SCHOOL OF PUBLIC HEALTH   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GENOMIC DNA;

ARTICLE; BACTERIAL CAPSULE LOCUS; BACTERIAL GENE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BEXA GENE; BEXB GENE; COMMENSAL; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA MICROARRAY; DNA SEQUENCE; GENE IDENTIFICATION; HAEMOPHILUS INFLUENZAE; NONHUMAN; NUCLEOTIDE SEQUENCE; OTITIS MEDIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SEROTYPE; STRAIN DIFFERENCE; STRAIN IDENTIFICATION; VALIDATION PROCESS;

BACTERIAL CAPSULES; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; DNA, BACTERIAL; HAEMOPHILUS INFLUENZAE; HUMANS; MEMBRANE TRANSPORT PROTEINS; MICROARRAY ANALYSIS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

HAEMOPHILUS INFLUENZAE;

EID: 79959841851     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02509-10     Document Type: Article

References (47)

1. Anderson, P., J. Pitt, and D. H. Smith. 1976. Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro. Infect. Immun. 13:581-589.
2. CDC. 2002. Serotyping discrepancies in Haemophilus influenzae type b disease, United States, 1998-1999. MMWR Morb. Mortal. Wkly. Rep. 51:706-707.
3. Cerquetti, M., et al. 2005. Presence of multiple copies of the capsulation b locus in invasive Haemophilus influenzae type b (Hib) strains isolated from children with Hib conjugate vaccine failure. J. Infect. Dis. 192:819-823.
4. Cerquetti, M., et al. 2006. Genetic diversity of invasive strains of Haemophilus influenzae type b before and after introduction of the conjugate vaccine in Italy. Clin. Infect. Dis. 43:317-319.
5. Cerquetti, M., et al. 2000. Characterization of non-type b Haemophilus influenzae strains isolated from patients with invasive disease. The HI Study Group. J. Clin. Microbiol. 38:4649-4652.
6. Ecevit, I. Z., et al. 2004. Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia genes in Haemophilus influenzae isolates. J. Clin. Microbiol. 42:3065-3072.
7. Eck, R., and M. O. Dayhoff. 1966. Atlas of protein sequence and structure. National Biomedical Research Foundation, Silver Spring, MD.
8. Falla, T. J., et al. 1994. PCR for capsular typing of Haemophilus influenzae. J. Clin. Microbiol. 32:2382-2386.
9. Farjo, R. S., et al. 2004. Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers. Pediatr. Infect. Dis. J. 23:41-46.
10. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
11. Fleischmann, R. D., et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
12. Giufre, M., R. Cardines, P. Mastrantonio, and M. Cerquetti. 2006. Variant IS1016 insertion elements in invasive Haemophilus influenzae type b isolates harboring multiple copies of the capsulation b locus. Clin. Infect. Dis. 43:1225-1226.
13. Harrison, A., et al. 2005. Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae: comparative study with H. influenzae serotype d, strain KW20. J. Bacteriol. 187:4627-4636. (Pubitemid 40880975)
14. Hoiseth, S. K., C. J. Connelly, and E. R. Moxon. 1985. Genetics of spontaneous, high-frequency loss of b capsule expression in Haemophilus influenzae. Infect. Immun. 49:389-395.
15. Hoiseth, S. K., and J. R. Gilsdorf. 1988. The relationship between type b and nontypable Haemophilus influenzae isolated from the same patient. J. Infect. Dis. 158:643-645.
16. Hoiseth, S. K., E. R. Moxon, and R. P. Silver. 1986. Genes involved in Haemophilus influenzae type b capsule expression are part of an 18-kilobase tandem duplication. Proc. Natl. Acad. Sci. U. S. A. 83:1106-1110.
17. Kapogiannis, B. G., S. Satola, H. L. Keyserling, and M. M. Farley. 2005. Invasive infections with Haemophilus influenzae serotype a containing an IS1016-bexA partial deletion: possible association with virulence. Clin. Infect. Dis. 41:e97-e103.
18. Kong, F., M. Brown, A. Sabananthan, X. Zeng, and G. L. Gilbert. 2006. Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes. J. Clin. Microbiol. 44:1887-1891.
19. Kroll, J. S., I. Hopkins, and E. R. Moxon. 1988. Capsule loss in H. influenzae type b occurs by recombination-mediated disruption of a gene essential for polysaccharide export. Cell 53:347-356.
20. Kroll, J. S., B. Loynds, L. N. Brophy, and E. R. Moxon. 1990. The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Mol. Microbiol. 4:1853-1862.
21. Kroll, J. S., B. M. Loynds, and E. R. Moxon. 1991. The Haemophilus influenzae capsulation gene cluster: a compound transposon. Mol. Microbiol. 5:1549-1560.
22. Kroll, J. S., and E. R. Moxon. 1988. Capsulation and gene copy number at the cap locus of Haemophilus influenzae type b. J. Bacteriol. 170:859-864.
23. Kroll, J. S., S. Zamze, B. Loynds, and E. R. Moxon. 1989. Common organization of chromosomal loci for production of different capsular polysaccharides in Haemophilus influenzae. J. Bacteriol. 171:3343-3347.
24. LaClaire, L. L., et al. 2003. Identification of Haemophilus influenzae serotypes by standard slide agglutination serotyping and PCR-based capsule typing. J. Clin. Microbiol. 41:393-396.
25. Lacross, N. C., C. F. Marrs, M. Patel, S. A. Sandstedt, and J. R. Gilsdorf. 2008. High genetic diversity of nontypeable Haemophilus influenzae isolates from two children attending a day care center. J. Clin. Microbiol. 46:3817-3821.
26. Maaroufi, Y., J. M. De Bruyne, C. Heymans, and F. Crokaert. 2007. Realtime PCR for determining capsular serotypes of Haemophilus influenzae. J. Clin. Microbiol. 45:2305-2308.
27. McCrea, K. W., J. L. Sauver, C. F. Marrs, D. Clemans, and J. R. Gilsdorf. 1998. Immunologic and structural relationships of the minor pilus subunits among Haemophilus influenzae isolates. Infect. Immun. 66:4788-4796.
28. McCrea, K. W., et al. 2010. Prevalence of the sodC gene in nontypeable Haemophilus influenzae and Haemophilus haemolyticus by microarray-based hybridization. J. Clin. Microbiol. 48:714-719.
29. Mukundan, D., Z. Ecevit, M. Patel, C. F. Marrs, and J. R. Gilsdorf. 2007. Pharyngeal colonization dynamics of Haemophilus influenzae and Haemophilus haemolyticus in healthy adult carriers. J. Clin. Microbiol. 45:3207-3217.
30. Murphy, T. F., and M. A. Apicella. 1987. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev. Infect. Dis. 9:1-15.
31. Nelson, K. L., and A. L. Smith. 2010. Determination of capsulation status in Haemophilus influenzae by multiplex polymerase chain reaction. Diagn. Microbiol. Infect. Dis. 66:235-240.
32. Ogilvie, C., et al. 2001. Capsulation loci of non-serotype b encapsulated Haemophilus influenzae. J. Infect. Dis. 184:144-149.
33. Pittman, M. 1931. Variation and type specificity in the bacterial species Haemophilus influenzae. J. Exp. Med. 53:471-492.
34. Saeed, A. I., et al. 2003. TM4: a free, open-source system for microarray data management and analysis. Biotechniques 34:374-378. (Pubitemid 36184095)
35. Sam, I. C., and M. Smith. 2005. Failure to detect capsule gene bexA in Haemophilus influenzae types e and f by real-time PCR due to sequence variation within probe binding sites. J. Med. Microbiol. 54:453-455.
36. Sandstedt, S. A., et al. 2008. Comparison of laboratory-based and phylogenetic methods to distinguish between Haemophilus influenzae and H. haemolyticus. J. Microbiol. Methods 75:369-371.
37. Satola, S. W., P. L. Schirmer, and M. M. Farley. 2003. Complete sequence of the cap locus of Haemophilus influenzae serotype b and nonencapsulated b capsule-negative variants. Infect. Immun. 71:3639-3644.
38. Satola, S. W., P. L. Schirmer, and M. M. Farley. 2003. Genetic analysis of the capsule locus of Haemophilus influenzae serotype f. Infect. Immun. 71:7202-7207.
39. St. Sauver, J., et al. 2000. Risk factors for otitis media and carriage of multiple strains of Haemophilus influenzae and Streptococcus pneumoniae. Emerg. Infect. Dis. 6:622-630.
40. Sukupolvi-Petty, S., S. Grass, and J. W. St. Geme III. 2006. The Haemophilus influenzae type b hcsA and hcsB gene products facilitate transport of capsular polysaccharide across the outer membrane and are essential for virulence. J. Bacteriol. 188:3870-3877.
41. Tamura, K., J. Dudley, M. Nei, and S. Kumar. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599.
42. Tamura, K., M. Nei, and S. Kumar. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. U. S. A. 101:11030-11035.
43. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
44. Ueno, K., J. Nishi, N. Imuta, K. Tokuda, and Y. Kawano. 2010. Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae type b (Hib) strains in Japan before introduction of the Hib conjugate vaccine. Microbiol. Immunol. 54:160-163.
45. Wallace, R. J., Jr., et al. 1981. Haemophilus influenzae infections in adults: characterization of strains by serotypes, biotypes, and beta-lactamase production. J. Infect. Dis. 144:101-106.
46. Zhang, L., et al. 2004. Library on a slide for bacterial comparative genomics. BMC Microbiol. 4:12.
47. Zhou, J., D. K. Law, M. L. Sill, and R. S. Tsang. 2007. Nucleotide sequence diversity of the bexA gene in serotypeable Haemophilus influenzae strains recovered from invasive disease patients in Canada. J. Clin. Microbiol. 45:1996-1999.