-
1
-
-
74049101638
-
Lipase-mediated enantioselective kinetic resolution of racemic acidic drugs in non-standard organic solvents: Direct chiral liquid chromatography monitoring and accurate determination of the enantiomeric excesses
-
Feb 12
-
Ghanem A, Aboul-Enein MN, El-Azzouny A, El-Behairy MF. Lipase-mediated enantioselective kinetic resolution of racemic acidic drugs in non-standard organic solvents: Direct chiral liquid chromatography monitoring and accurate determination of the enantiomeric excesses. J Cromatogr A. 2010 Feb 12; 1217 (7): 1063-1074.
-
(2010)
J Cromatogr A
, vol.1217
, Issue.7
, pp. 1063-1074
-
-
Ghanem, A.1
Aboul-Enein, M.N.2
El-Azzouny, A.3
El-Behairy, M.F.4
-
2
-
-
77956893668
-
Lipase-mediated preparation of optically active isomers of Rosaphen
-
Nov
-
Kawasaki M, Toyooka N, Saka T, Goto M, Matsuya Y, Kometani T. Lipase-mediated preparation of optically active isomers of Rosaphen. J Mol Catal B-Enzym. 2010 Nov; 67 (1-2): 135-142.
-
(2010)
J Mol Catal B-Enzym
, vol.67
, Issue.1-2
, pp. 135-142
-
-
Kawasaki, M.1
Toyooka, N.2
Saka, T.3
Goto, M.4
Matsuya, Y.5
Kometani, T.6
-
3
-
-
7444245720
-
Lipase-mediated chiral resolution of racemates in organic solvents
-
Nov 1
-
Ghanem A, Aboul-Enein HY. Lipase-mediated chiral resolution of racemates in organic solvents. Tetrahedron-Asymmetr. 2004 Nov 1; 15 (21): 3331-3351.
-
(2004)
Tetrahedron-Asymmetr
, vol.15
, Issue.21
, pp. 3331-3351
-
-
Ghanem, A.1
Aboul-Enein, H.Y.2
-
4
-
-
77949915679
-
Lipase resolution of new (±)-3-aryloxy-1-halogenopropan-2-ols: Versatilebuilding blocks for β-adrenergic receptor antagonists
-
Mar
-
Maciejewski M, Półtorak K, Kaminska JE. Lipase resolution of new (±)-3-aryloxy-1-halogenopropan-2-ols: Versatilebuilding blocks for β-adrenergic receptor antagonists. J Mol Catal B-Enzym. 2010 Mar; 62 (3-4): 248-256.
-
(2010)
J Mol Catal B-Enzym
, vol.62
, Issue.3-4
, pp. 248-256
-
-
McIejewski, M.1
Półtorak, K.2
Kaminska, J.E.3
-
5
-
-
0027160452
-
Insights into interfacial activation from an open structure of Candida rugosa lipase
-
Jun 15
-
Grochulski P, Li Y, Schrag JD, Bouthillier F, Smith P, Harrison D, et al. Insights into interfacial activation from an open structure of Candida rugosa lipase. J Biol Chem. 1993 Jun 15; 268 (17): 12843-12847.
-
(1993)
J Biol Chem
, vol.268
, Issue.17
, pp. 12843-12847
-
-
Grochulski, P.1
Li, Y.2
Schrag, J.D.3
Bouthillier, F.4
Smith, P.5
Harrison, D.6
-
6
-
-
0032104742
-
Immobilization of lipases by selective adsorption on hydrophobic supports
-
Jun
-
Fernández-Lafuente R, Armisén P, Sabuquillo P, Fernández-Lorente G, Guisán JM. Immobilization of lipases by selective adsorption on hydrophobic supports. Chem Phys Lipids. 1998 Jun; 93 (1-2): 185-197.
-
(1998)
Chem Phys Lipids
, vol.93
, Issue.1-2
, pp. 185-197
-
-
Fernández-Lafuente, R.1
Armisén, P.2
Sabuquillo, P.3
Fernández-Lorente, G.4
Guisán, J.M.5
-
7
-
-
0034575018
-
Multifunctional epoxy supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage
-
Nov 10
-
Mateo C, Fernández-Lorente G, Abian O, Fernández-Lafuente R, Guisán JM. Multifunctional epoxy supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules. 2000 Nov 10; 1 (4): 739-745.
-
(2000)
Biomacromolecules
, vol.1
, Issue.4
, pp. 739-745
-
-
Mateo, C.1
Fernández-Lorente, G.2
Abian, O.3
Fernández-Lafuente, R.4
Guisán, J.M.5
-
8
-
-
0035826279
-
Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: Enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase
-
Mar 8
-
Fernández-Lorente G, Terreni M, Mateo C, Bastida A, Fernández-Lafuente R, Dalmases P, et al. Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase. Enzyme Microb Tech. 2001 Mar 8; 28 (4-5): 389-396.
-
(2001)
Enzyme Microb Tech
, vol.28
, Issue.4-5
, pp. 389-396
-
-
Fernández-Lorente, G.1
Terreni, M.2
Mateo, C.3
Bastida, A.4
Fernández-Lafuente, R.5
Dalmases, P.6
-
9
-
-
0035931364
-
Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium
-
Jan 22
-
Fernández-Lorente G, Fernández-Lafuente R, Palomo JM, Mateo C, Bastida A, Coca J, et al. Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium. J Mol Catal B-Enzym. 2001 Jan 22; 11 (4-6): 649-656.
-
(2001)
J Mol Catal B-Enzym
, vol.11
, Issue.4-6
, pp. 649-656
-
-
Fernández-Lorente, G.1
Fernández-Lafuente, R.2
Palomo, J.M.3
Mateo, C.4
Bastida, A.5
Coca, J.6
-
10
-
-
1842474522
-
Enzymatic resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques
-
Apr 5
-
Palomo JM, Segura RL, Fernández-Lorente G, Guisán JM, Fernández-Lafuente R. Enzymatic resolution of (±)-glycidyl butyrate in aqueous media. Strong modulation of the properties of the lipase from Rhizopus oryzae via immobilization techniques. Tetrahedron-Asymmetr. 2004 Apr 5; 15 (7): 1157-1161.
-
(2004)
Tetrahedron-Asymmetr
, vol.15
, Issue.7
, pp. 1157-1161
-
-
Palomo, J.M.1
Segura, R.L.2
Fernández-Lorente, G.3
Guisán, J.M.4
Fernández-Lafuente, R.5
-
11
-
-
0037450167
-
Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins: Hydrolytic resolution of (RS)-2-butyroyl-2-phenylacetic acid
-
Feb 17
-
Palomo JM, Muñoz G, Fernández-Lorente G, Mateo C, Fuentes M, Guisán JM, et al. Modulation of Mucor miehei lipase properties via directed immobilization on different hetero-functional epoxy resins: Hydrolytic resolution of (RS)-2-butyroyl-2-phenylacetic acid. J Mol Catal B-Enzym. 2003 Feb 17; 21 (4-6): 201-210.
-
(2003)
J Mol Catal B-Enzym
, vol.21
, Issue.4-6
, pp. 201-210
-
-
Palomo, J.M.1
Muñoz, G.2
Fernández-Lorente, G.3
Mateo, C.4
Fuentes, M.5
Guisán, J.M.6
-
12
-
-
0037158235
-
Pseudomonas sp. lipase immobilized in polymers versus the use of free enzyme in the resolution of (R, S)-methyl mandelate
-
Jul 22
-
Queiroz N, Nascimento MG. Pseudomonas sp. lipase immobilized in polymers versus the use of free enzyme in the resolution of (R, S)-methyl mandelate. Tetrahedron Lett. 2002 Jul 22; 43 (30): 5225-5227.
-
(2002)
Tetrahedron Lett
, vol.43
, Issue.30
, pp. 5225-5227
-
-
Queiroz, N.1
Nascimento, M.G.2
-
13
-
-
67349227664
-
Enhancement of the activity and enantioselectivity of lipase in organic systems by immobilization onto low-cost support
-
May
-
Yang G, Wu J, Xu G, Yang L. Enhancement of the activity and enantioselectivity of lipase in organic systems by immobilization onto low-cost support. J Mol Catal B-Enzym. 2009 May; 57 (1-4): 96-103.
-
(2009)
J Mol Catal B-Enzym
, vol.57
, Issue.1-4
, pp. 96-103
-
-
Yang, G.1
Wu, J.2
Xu, G.3
Yang, L.4
-
14
-
-
73949089314
-
Effect of a buffer mixture system on the activity of lipases during immobilization process
-
Jan
-
Lee JH, Kim SB, Park Ch, Kim SW. Effect of a buffer mixture system on the activity of lipases during immobilization process. Bioresour Technol. 2010 Jan; 101 (1 Suppl 1): S66-S70.
-
(2010)
Bioresour Technol
, vol.101
, Issue.1 SUPPL. 1
-
-
Lee, J.H.1
Kim, S.B.2
Park, C.3
Kim, S.W.4
-
15
-
-
0036843441
-
Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: Hydrolytic resolution of mandelic acid esters
-
Nov 1
-
Palomo JM, Fernandez-Lorente G, Mateo C, Ortiz C, Fernández-Lafuente R, Guisan JM. Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters. Enzyme Microb Tech. 2002 Nov 1; 31 (6): 775-783.
-
(2002)
Enzyme Microb Tech
, vol.31
, Issue.6
, pp. 775-783
-
-
Palomo, J.M.1
Fernandez-Lorente, G.2
Mateo, C.3
Ortiz, C.4
Fernández-Lafuente, R.5
Guisan, J.M.6
-
16
-
-
0037025268
-
Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering. Kinetic resolution of (±)-α-hydroxy-phenylacetic acid derivatives
-
Jul 5
-
Palomo JM, Fernández-Lorente G, Mateo C, Fuentes M, Fernández-Lafuente R, Guisan JM. Modulation of the enantioselectivity of Candida antarctica B lipase via conformational engineering. Kinetic resolution of (±)-α-hydroxy-phenylacetic acid derivatives. Tetrahedron-Asymmetr. 2002 Jul 5; 13 (12): 1337-1345.
-
(2002)
Tetrahedron-Asymmetr
, vol.13
, Issue.12
, pp. 1337-1345
-
-
Palomo, J.M.1
Fernández-Lorente, G.2
Mateo, C.3
Fuentes, M.4
Fernández-Lafuente, R.5
Guisan, J.M.6
-
17
-
-
54749090797
-
Lipa ses enant ioselect ivit y a lterat ion by immobilization techniques
-
Aug
-
Palomo JM. Lipa ses enant ioselect ivit y a lterat ion by immobilization techniques. Curr Bioact Compd. 2008 Aug; 4 (2): 126-138.
-
(2008)
Curr Bioact Compd
, vol.4
, Issue.2
, pp. 126-138
-
-
Palomo, J.M.1
-
18
-
-
0017184389
-
A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
-
May 7
-
Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7; 72 (1-2): 248-254.
-
(1976)
Anal Biochem
, vol.72
, Issue.1-2
, pp. 248-254
-
-
Bradford, M.M.1
-
20
-
-
0032506270
-
Interfacial affinity chromatography of lipases: Separation of different fractions by selective adsorption on supports activated with hydrophobics groups
-
Nov 10
-
Sabuquillo P, Reina J, Fernández-Lorente G, Guisán JM, Fernández-Lafuente R. Interfacial affinity chromatography of lipases: separation of different fractions by selective adsorption on supports activated with hydrophobics groups. Biochim Biophys Acta. 1998 Nov 10; 1388 (2): 337-348.
-
(1998)
Biochim Biophys Acta
, vol.1388
, Issue.2
, pp. 337-348
-
-
Sabuquillo, P.1
Reina, J.2
Fernández-Lorente, G.3
Guisán, J.M.4
Fernández-Lafuente, R.5
-
21
-
-
0032486523
-
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrofobic supports
-
Jun 5
-
Bastida A, Sabuquillo P, Armisén P, Fernández-Lafuente R, Huguet J, Guisan JM. A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrofobic supports. Biotechnol Bioeng. 1998 Jun 5; 58 (5): 486-493.
-
(1998)
Biotechnol Bioeng
, vol.58
, Issue.5
, pp. 486-493
-
-
Bastida, A.1
Sabuquillo, P.2
Armisén, P.3
Fernández-Lafuente, R.4
Huguet, J.5
Guisan, J.M.6
-
22
-
-
28844483422
-
Purification of different lipases from Aspergillus niger using a highly selective adsorption on hydrophobic supports
-
Dic 20
-
Fernández-Lorente G, Ortiz C, Segura RL, Fernández-Lafuente R, Guisán JM, Palomo JM. Purification of different lipases from Aspergillus niger using a highly selective adsorption on hydrophobic supports. Biotechnol Bioeng. 2005 Dic 20; 92 (6): 773-779.
-
(2005)
Biotechnol Bioeng
, vol.92
, Issue.6
, pp. 773-779
-
-
Fernández-Lorente, G.1
Ortiz, C.2
Segura, R.L.3
Fernández-Lafuente, R.4
Guisán, J.M.5
Palomo, J.M.6
-
23
-
-
0032982259
-
Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports
-
Jul 2
-
Armisén P, Mateo C, Cortés E, Barredo JL, Salto F, Diez B, et al. Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports. J Chromatogr A. 1995 Jul 2; 848 (1-2): 61-70.
-
(1995)
J Chromatogr A
, vol.848
, Issue.1-2
, pp. 61-70
-
-
Armisén, P.1
Mateo, C.2
Cortés, E.3
Barredo, J.L.4
Salto, F.5
Diez, B.6
-
24
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Aug 15
-
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15; 227 (5259): 680-685.
-
(1970)
Nature
, vol.227
, Issue.5259
, pp. 680-685
-
-
Laemmli, U.K.1
-
25
-
-
0037010728
-
Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): Immobilization, hyperactivation and stabilization of the open form of lipases
-
Dec 2
-
Palomo JM, Muñoz G, Fernández-Lorente G, Mateo C, Fernández-Lafuente R, Guisán, JM. Interfacial adsorption of lipases on very hydrophobic support (octadecyl-Sepabeads): immobilization, hyperactivation and stabilization of the open form of lipases. J Mol Catal B-Enzym. 2002 Dec 2; 19-20: 279-286.
-
(2002)
J Mol Catal B-Enzym
, vol.19-20
, pp. 279-286
-
-
Palomo, J.M.1
Muñoz, G.2
Fernández-Lorente, G.3
Mateo, C.4
Fernández-Lafuente, R.5
Guisán, J.M.6
-
26
-
-
0035149580
-
The effect of ethanol on the kinetics of lipase-mediated enantioselective esterification of 4-methyloctanoic acid and the hydrolysis of its ethyl ester
-
Nov
-
Heinsman NWJT, Valente AM, Smienk HGF, Van der Padt A, Franssen MCR, De Groot A, et al. The effect of ethanol on the kinetics of lipase-mediated enantioselective esterification of 4-methyloctanoic acid and the hydrolysis of its ethyl ester. Biotechnol Bioeng. 2001 Nov; 76 (3): 193-199.
-
(2001)
Biotechnol Bioeng
, vol.76
, Issue.3
, pp. 193-199
-
-
Heinsman, N.W.J.T.1
Valente, A.M.2
Smienk, H.G.F.3
van der Padt, A.4
Franssen, M.C.R.5
De Groot, A.6
-
27
-
-
0037457503
-
Self-assembly of Pseudomonas f luorescens lipase into bimolecular aggregates dramatically affects functional properties
-
Apr 20
-
Fernández-Lorente G, Palomo JM, Fuentes M, Mateo C, Guisán JM, Fernández-Lafuente R. Self-assembly of Pseudomonas f luorescens lipase into bimolecular aggregates dramatically affects functional properties. Biotechnol Bioeng. 2003 Apr 20; 82 (2): 232-237.
-
(2003)
Biotechnol Bioeng
, vol.82
, Issue.2
, pp. 232-237
-
-
Fernández-Lorente, G.1
Palomo, J.M.2
Fuentes, M.3
Mateo, C.4
Guisán, J.M.5
Fernández-Lafuente, R.6
-
28
-
-
33646535438
-
Effect of lipase-lipase interactions in the activity, stability and specificity of a lipase from Alcaligenes sp
-
Jun 26
-
Wilson L, Palomo JM, Fernández-Lorente G, Illanes A, Guisán JM, Fernández-Lafuente R. Effect of lipase-lipase interactions in the activity, stability and specificity of a lipase from Alcaligenes sp. Enzyme Microbial Tech. 2006 Jun 26; 39 (2): 259-264.
-
(2006)
Enzyme Microbial Tech
, vol.39
, Issue.2
, pp. 259-264
-
-
Wilson, L.1
Palomo, J.M.2
Fernández-Lorente, G.3
Illanes, A.4
Guisán, J.M.5
Fernández-Lafuente, R.6
-
29
-
-
34047128801
-
Stability of biocatalysts
-
Apr
-
Polizzi KM, Bommarius AS, Broering JM, Chaparro-Riggers JF. Stability of biocatalysts. Curr Opin Chem Biol. 2007 Apr; 11 (2): 220-225.
-
(2007)
Curr Opin Chem Biol
, vol.11
, Issue.2
, pp. 220-225
-
-
Polizzi, K.M.1
Bommarius, A.S.2
Broering, J.M.3
Chaparro-Riggers, J.F.4
-
30
-
-
0017268663
-
Kinetic behavior of immobilized enzyme Systems
-
Goldstein L. Kinetic behavior of immobilized enzyme Systems. Methods Enzymol. 1976; 44: 397-443.
-
(1976)
Methods Enzymol
, vol.44
, pp. 397-443
-
-
Goldstein, L.1
|