Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems

BMC Developmental Biology

Volumn 11, Issue , 2011, Pages

  Lauter, Gilbert ^a   Söll, Iris ^a   Hauptmann, Giselbert ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

alkaline phosphatase; dextran sulfate; digoxigenin; dinitrophenol; Fast Blue; Fast Red; horseradish peroxidase; hydrogen peroxide; permeabilization; prosomere; tyramide signal amplification (TSA)

Indexed keywords

ARMORACIA RUSTICANA; DANIO RERIO;

6 CARBOXYFLUORESCEIN; 6-CARBOXYFLUORESCEIN; ALKALINE PHOSPHATASE; FLUORESCEIN DERIVATIVE; HORSERADISH PEROXIDASE;

ANIMAL; ANIMAL EMBRYO; ARTICLE; BRAIN; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY; PRENATAL DEVELOPMENT; ZEBRA FISH;

ALKALINE PHOSPHATASE; ANIMALS; BRAIN; EMBRYO, NONMAMMALIAN; FLUORESCEINS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HORSERADISH PEROXIDASE; IN SITU HYBRIDIZATION, FLUORESCENCE; ZEBRAFISH;

EID: 79959795419     PISSN: None     EISSN: 1471213X     Source Type: Journal    
DOI: 10.1186/1471-213X-11-43     Document Type: Article

References (42)

1. An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex. E Hafen M Levine RL Garber WJ Gehring, EMBO J 1983 2 617 23 16453446
2. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. D Tautz C Pfeifle, Chromosoma 1989 98 81 5 10.1007/BF00291041 2476281 (Pubitemid 19205971)
3. Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies. G Hauptmann, Dev Genes Evol 1999 209 317 21 10.1007/s004270050258 11252185 (Pubitemid 29206353)
4. One-, two-, and three-color whole-mount in situ hybridization to Drosophila embryos. G Hauptmann, Methods 2001 23 359 72 10.1006/meth.2000.1148 11316437 (Pubitemid 32846455)
5. Multicolor whole-mount in situ hybridization. G Hauptmann T Gerster, Methods Mol Biol 2000 137 139 48 10948532
6. Double in situ hybridization techniques in zebrafish. T Jowett, Methods 2001 23 345 58 10.1006/meth.2000.1147 11316436 (Pubitemid 32846454)
7. Double-label in situ hybridization using biotin and digoxigenin-tagged RNA probes. JW O'Neill E Bier, Biotechniques 1994 17 870, 874 5
8. Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. G Hauptmann T Gerster, Trends Genet 1994 10 266 7940754 (Pubitemid 24238896)
9. Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. T Jowett L Lettice, Trends Genet 1994 10 73 4 10.1016/0168-9525(94)90220-8 8178366
10. Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain. G Hauptmann T Gerster, Mech Dev 2000 91 105 18 10.1016/S0925-4773(99)00277-4 10704836 (Pubitemid 30125910)
11. The early embryonic zebrafish forebrain is subdivided into molecularly distinct transverse and longitudinal domains. G Hauptmann I Söll T Gerster, Brain Res Bull 2002 57 371 5 10.1016/S0361-9230(01)00691-8 11922991 (Pubitemid 34234889)
12. spiel ohne grenzen/pou2 is required for zebrafish hindbrain segmentation. G Hauptmann HG Belting U Wolke K Lunde I Söll S Abdelilah-Seyfried V Prince W Driever, Development 2002 129 1645 55 11923201 (Pubitemid 34874182)
13. Distribution of corticotropin-releasing hormone in the developing zebrafish brain. G Chandrasekar G Lauter G Hauptmann, J Comp Neurol 2007 505 337 51 10.1002/cne.21496 17912740 (Pubitemid 350134057)
14. Localized expression of urocortin genes in the developing zebrafish brain. L Bräutigam JM Hillmer I Söll G Hauptmann, J Comp Neurol 2010 518 2978 95 10.1002/cne.22375 20533356
15. Alkaline phosphatase-fast red, a new fluorescent label. Application in double labelling for cell cycle analysis. A Murdoch EJ Jenkinson GD Johnson JJ Owen, J Immunol Methods 1990 132 45 9 10.1016/0022-1759(90)90396-D 2118160 (Pubitemid 20263860)
16. Use of a new fluorogenic phosphatase substrate in immunohistochemical applications. KD Larison R BreMiller KS Wells I Clements RP Haugland, J Histochem Cytochem 1995 43 77 83 10.1177/43.1.7822768 7822768
17. Double in situ hybridization on mouse embryos for detection of overlapping regions of gene expression. D Bueno J Skinner H Abud JK Heath, Trends Genet 1996 12 385 7 10.1016/S0168-9525(96)90090-6 8909126 (Pubitemid 26336472)
18. Double fluorescent in situ hybridization to zebrafish embryos. T Jowett YL Yan, Trends Genet 1996 12 387 9 10.1016/S0168-9525(96)90091-8 8909127 (Pubitemid 26336473)
19. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. MN Bobrow TD Harris KJ Shaughnessy GJ Litt, J Immunol Methods 1989 125 279 85 10.1016/0022-1759(89)90104-X 2558138 (Pubitemid 20018033)
20. Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification. H Clay L Ramakrishnan, Zebrafish 2005 2 105 11 10.1089/zeb.2005.2.105 18248170 (Pubitemid 41335551)
21. FISHing for chick genes: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos. N Denkers P Garcia-Villalba CK Rodesch KR Nielson TJ Mauch, Dev Dyn 2004 229 651 7 10.1002/dvdy.20005 14991720 (Pubitemid 38294913)
22. Multiplex detection of RNA expression in Drosophila embryos. D Kosman CM Mizutani D Lemons WG Cox W McGinnis E Bier, Science 2004 305 846 10.1126/science.1099247 15297669 (Pubitemid 39038414)
23. Fluorescent two-color whole mount in situ hybridization in Platynereis dumerilii (Polychaeta, Annelida), an emerging marine molecular model for evolution and development. K Tessmar-Raible PR Steinmetz H Snyman M Hassel D Arendt, Biotechniques 2005 39 460, 462, 464 16235555
24. Fluorescent in situ hybridization protocols in Drosophila embryos and tissues. E Lecuyer N Parthasarathy HM Krause, Methods Mol Biol 2008 420 289 302 10.1007/978-1-59745-583-1-18 18641955
25. Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. G Lauter I Söll G Hauptmann, Neural Dev 2011 6 10 10.1186/1749-8104-6-10 21466670
26. Multicolour whole-mount in situ hybridization to Drosophila embryos. G Hauptmann T Gerster, Development Genes and Evolution 1996 206 292 295 10.1007/s004270050055 (Pubitemid 26402492)
27. Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol. RP van Gijlswijk J Wiegant AK Raap HJ Tanke, J Histochem Cytochem 1996 44 389 92 10.1177/44.4.8601698 8601698 (Pubitemid 26102436)
28. Implications of macromolecular crowding for protein assembly. AP Minton, Curr Opin Struct Biol 2000 10 34 9 10.1016/S0959-440X(99)00045-7 10679465 (Pubitemid 30099324)
29. Combinatorial expression of zebrafish Brn-1- and Brn-2-related POU genes in the embryonic brain, pronephric primordium, and pharyngeal arches. G Hauptmann T Gerster, Dev Dyn 2000 218 345 58 10.1002/(SICI)1097-0177(200006)218: 2<345::AID-DVDY8>3.0.CO;2-V 10842361 (Pubitemid 30349644)
30. A guided tour into subcellular colocalization analysis in light microscopy. S Bolte FP Cordelieres, J Microsc 2006 224 213 32 10.1111/j.1365-2818.2006.01706.x 17210054 (Pubitemid 46010930)
31. Expression patterns of homeobox and other putative regulatory genes in the embryonic mouse forebrain suggest a neuromeric organization. L Puelles JL Rubenstein, Trends Neurosci 1993 16 472 9 10.1016/0166-2236(93)90080-6 7507621 (Pubitemid 23313526)
32. Forebrain gene expression domains and the evolving prosomeric model. L Puelles JL Rubenstein, Trends Neurosci 2003 26 469 76 10.1016/S0166-2236(03) 00234-0 12948657 (Pubitemid 37025784)
33. Postembryonic neural proliferation in the zebrafish forebrain and its relationship to prosomeric domains. MF Wullimann L Puelles, Anat Embryol (Berl) 1999 199 329 48 10.1007/s004290050232 (Pubitemid 29137312)
34. Control of anteroposterior and dorsoventral domains of Nkx-6.1 gene expression relative to other Nkx genes during vertebrate CNS development. M Qiu K Shimamura L Sussel S Chen JL Rubenstein, Mech Dev 1998 72 77 88 10.1016/S0925-4773(98)00018-5 9533954 (Pubitemid 28186708)
35. Programmable in situ amplification for multiplexed imaging of mRNA expression. HM Choi JY Chang A Trinh le JE Padilla SE Fraser NA Pierce, Nat Biotechnol 2010 28 1208 12 10.1038/nbt.1692 21037591
36. In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. WP Kloosterman E Wienholds E de Bruijn S Kauppinen RH Plasterk, Nat Methods 2006 3 27 9 10.1038/nmeth843 16369549 (Pubitemid 43135345)
37. Pre-pattern in the pronephric kidney field of zebrafish. FC Serluca MC Fishman, Development 2001 128 2233 41 11493543 (Pubitemid 32633357)
38. A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. S Krauss JP Concordet PW Ingham, Cell 1993 75 1431 44 10.1016/0092-8674(93)90628-4 8269519 (Pubitemid 24021916)
39. Sequence and expression pattern of pax-6 are highly conserved between zebrafish and mice. AW Püschel P Gruss M Westerfield, Development 1992 114 643 51 1352238
40. Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation. SE Cheesman MJ Layden T Von Ohlen CQ Doe JS Eisen, Development 2004 131 5221 32 10.1242/dev.01397 15456722 (Pubitemid 39562168)
41. Sensitive two-color whole-mount in situ hybridizations using digoxygenin- and dinitrophenol-labeled RNA probes. S Long M Rebagliati, Biotechniques 2002 32 494 500 11911652 (Pubitemid 34208065)
42. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. AH Hopman FC Ramaekers EJ Speel, J Histochem Cytochem 1998 46 771 7 10.1177/002215549804600611 9603790 (Pubitemid 28272213)