Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain

Neural Development

Volumn 6, Issue 1, 2011, Pages

  Lauter, Gilbert ^a   Söll, Iris ^a   Hauptmann, Giselbert ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; DEXTRAN SULFATE; GLYCINE; HAPTEN; HORSERADISH PEROXIDASE; TYRAMINE;

ANIMAL; ARTICLE; BRAIN; BRAIN LEVEL; CHEMISTRY; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; IMAGE PROCESSING; METABOLISM; NUCLEIC ACID PROBE; PHYSIOLOGY; PRENATAL DEVELOPMENT; SIGNAL PROCESSING; SYNTHESIS; VISCOSITY; ZEBRA FISH;

ANIMALS; ANTIBODIES; ANTISENSE ELEMENTS (GENETICS); BRAIN; BRAIN CHEMISTRY; DEXTRAN SULFATE; GENE EXPRESSION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GLYCINE; HAPTENS; HORSERADISH PEROXIDASE; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, FLUORESCENCE; SIGNAL PROCESSING, COMPUTER-ASSISTED; TYRAMINE; VISCOSITY; ZEBRAFISH;

EID: 79953290332     PISSN: None     EISSN: 17498104     Source Type: Journal    
DOI: 10.1186/1749-8104-6-10     Document Type: Article

References (37)

1. Puelles L, Rubenstein JL. Expression patterns of homeobox and other putative regulatory genes in the embryonic mouse forebrain suggest a neuromeric organization. Trends Neurosci 1993, 16:472-479. 10.1016/0166-2236(93)90080-6, 7507621.
2. Puelles L, Rubenstein JL. Forebrain gene expression domains and the evolving prosomeric model. Trends Neurosci 2003, 26:469-476. 10.1016/S0166-2236(03)00234-0, 12948657.
3. Rubenstein JL, Martinez S, Shimamura K, Puelles L. The embryonic vertebrate forebrain: the prosomeric model. Science 1994, 266:578-580. 10.1126/science.7939711, 7939711.
4. Rubenstein JL, Puelles L. Homeobox gene expression during development of the vertebrate brain. Curr Top Dev Biol 1994, 29:1-63. full_text, 7828435.
5. Hauptmann G. Two-color detection of mRNA transcript localizations in fish and fly embryos using alkaline phosphatase and beta-galactosidase conjugated antibodies. Dev Genes Evol 1999, 209:317-321. 10.1007/s004270050258, 11252185.
6. Hauptmann G, Gerster T. Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. Trends Genet 1994, 10:266. 10.1016/0168-9525(90)90008-T, 7940754.
7. Hauptmann G, Gerster T. Multicolor whole-mount in situ hybridization. Methods Mol Biol 2000, 137:139-148.
8. Jowett T. Double in situ hybridization techniques in zebrafish. Methods 2001, 23:345-358. 10.1006/meth.2000.1147, 11316436.
9. Jowett T, Lettice L. Whole-mount in situ hybridizations on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. Trends Genet 1994, 10:73-74. 10.1016/0168-9525(94)90220-8, 8178366.
10. Hauptmann G, Gerster T. Regulatory gene expression patterns reveal transverse and longitudinal subdivisions of the embryonic zebrafish forebrain. Mech Dev 2000, 91:105-118. 10.1016/S0925-4773(99)00277-4, 10704836.
11. Hauptmann G, Gerster T. Combinatorial expression of zebrafish Brn-1- and Brn-2-related POU genes in the embryonic brain, pronephric primordium, and pharyngeal arches. Dev Dyn 2000, 218:345-358. 10.1002/(SICI)1097-0177(200006)218:2<345::AID-DVDY8>3.0.CO;2-V, 10842361.
12. Hauptmann G, Söll I, Gerster T. The early embryonic zebrafish forebrain is subdivided into molecularly distinct transverse and longitudinal domains. Brain Res Bull 2002, 57:371-375. 10.1016/S0361-9230(01)00691-8, 11922991.
13. Chandrasekar G, Lauter G, Hauptmann G. Distribution of corticotropin-releasing hormone in the developing zebrafish brain. J Comp Neurol 2007, 505:337-351. 10.1002/cne.21496, 17912740.
14. Holzschuh J, Hauptmann G, Driever W. Genetic analysis of the roles of Hh, FGF8, and nodal signaling during catecholaminergic system development in the zebrafish brain. J Neurosci 2003, 23:5507-5519.
15. Luengo Hendriks CL, Keranen SV, Fowlkes CC, Simirenko L, Weber GH, DePace AH, Henriquez C, Kaszuba DW, Hamann B, Eisen MB, Malik J, Sudar D, Biggin MD, Knowles DW. Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution I: data acquisition pipeline. Genome Biol 2006, 7:R123. 10.1186/gb-2006-7-12-r123, 1794436, 17184546.
16. Denkers N, Garcia-Villalba P, Rodesch CK, Nielson KR, Mauch TJ. FISHing for chick genes: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos. Dev Dyn 2004, 229:651-657. 10.1002/dvdy.20005, 14991720.
17. Kosman D, Mizutani CM, Lemons D, Cox WG, McGinnis W, Bier E. Multiplex detection of RNA expression in Drosophila embryos. Science 2004, 305:846. 10.1126/science.1099247, 15297669.
18. Tessmar-Raible K, Steinmetz PR, Snyman H, Hassel M, Arendt D. Fluorescent two-color whole mount in situ hybridization in Platynereis dumerilii (Polychaeta, Annelida), an emerging marine molecular model for evolution and development. Biotechniques 2005, 39:460. 462, 464, 10.2144/000112023, 16235555.
19. Vize PD, McCoy KE, Zhou X. Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos. Nat Protoc 2009, 4:975-983. 10.1038/nprot.2009.69, 19498377.
20. Clay H, Ramakrishnan L. Multiplex fluorescent in situ hybridization in zebrafish embryos using tyramide signal amplification. Zebrafish 2005, 2:105-111. 10.1089/zeb.2005.2.105, 18248170.
21. Welten MC, de Haan SB, van den Boogert N, Noordermeer JN, Lamers GE, Spaink HP, Meijer AH, Verbeek FJ. ZebraFISH: fluorescent in situ hybridization protocol and three-dimensional imaging of gene expression patterns. Zebrafish 2006, 3:465-476. 10.1089/zeb.2006.3.465, 18377226.
22. Ellis RJ. Macromolecular crowding: obvious but underappreciated. Trends Biochem Sci 2001, 26:597-604. 10.1016/S0968-0004(01)01938-7, 11590012.
23. van Gijlswijk RP, Wiegant J, Raap AK, Tanke HJ. Improved localization of fluorescent tyramides for fluorescence in situ hybridization using dextran sulfate and polyvinyl alcohol. J Histochem Cytochem 1996, 44:389-392. 10.1177/44.4.8601698, 8601698.
24. Straus W. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH. J Histochem Cytochem 1982, 30:491-493. 10.1177/30.5.6176617, 6176617.
25. Stout RL. Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations. US Patent 4521511 1985, Overland Park, KS: Enzyme Technology Company.
26. Hopman AH, Ramaekers FC, Speel EJ. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem 1998, 46:771-777. 10.1177/002215549804600611, 9603790.
27. Hauptmann G. One-, two-, and three-color whole-mount in situ hybridization to Drosophila embryos. Methods 2001, 23:359-372. 10.1006/meth.2000.1148, 11316437.
28. Hauptmann G, Gerster T. Multicolour whole-mount in situ hybridization to Drosophila embryos. Dev Genes Evol 1996, 206:292-295.
29. Liu G, Amin S, Okuhama NN, Liao G, Mingle LA. A quantitative evaluation of peroxidase inhibitors for tyramide signal amplification mediated cytochemistry and histochemistry. Histochem Cell Biol 2006, 126:283-291. 10.1007/s00418-006-0161-x, 1713225, 16508759.
30. Fjose A, Izpisua-Belmonte JC, Fromental-Ramain C, Duboule D. Expression of the zebrafish gene hlx-1 in the prechordal plate and during CNS development. Development 1994, 120:71-81.
31. Cheesman SE, Eisen JS. gsh1 demarcates hypothalamus and intermediate spinal cord in zebrafish. Gene Expr Patterns 2004, 5:107-112. 10.1016/j.modgep.2004.05.006, 15533825.
32. Ando H, Kobayashi M, Tsubokawa T, Uyemura K, Furuta T, Okamoto H. Lhx2 mediates the activity of Six3 in zebrafish forebrain growth. Dev Biol 2005, 287:456-468. 10.1016/j.ydbio.2005.09.023, 16226737.
33. Morita T, Nitta H, Kiyama Y, Mori H, Mishina M. Differential expression of two zebrafish emx homeoprotein mRNAs in the developing brain. Neurosci Lett 1995, 198:131-134. 10.1016/0304-3940(95)11988-9, 8592638.
34. Krauss S, Concordet JP, Ingham PW. A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. Cell 1993, 75:1431-1444. 10.1016/0092-8674(93)90628-4, 8269519.
35. Akimenko MA, Ekker M, Wegner J, Lin W, Westerfield M. Combinatorial expression of three zebrafish genes related to distal-less: part of a homeobox gene code for the head. J Neurosci 1994, 14:3475-3486.
36. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. Stages of embryonic development of the zebrafish. Dev Dyn 1995, 203:253-310. 10.1002/aja.1002030302, 8589427.
37. Cheesman SE, Layden MJ, Von Ohlen T, Doe CQ, Eisen JS. Zebrafish and fly Nkx6 proteins have similar CNS expression patterns and regulate motoneuron formation. Development 2004, 131:5221-5232. 10.1242/dev.01397, 15456722.