Glu659Leu substitution of recombinant HIV fusion inhibitor C52L induces soluble expression in Escherichia coli with equivalent anti-HIV potency

Protein Engineering, Design and Selection

Volumn 24, Issue 7, 2011, Pages 545-551

  Shang, Shufeng ^a   Tan, Suiyi ^b   Li, Kangbai ^a   Wu, Jun ^a   Lin, Huina ^a   Liu, Shuwen ^b   Deng, Yiqun ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

^b SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

6 helix bundle (HB); gp41; HIV fusion inhibitor; leucine scanning mutagenesis; solubility

Indexed keywords

6-HELIX BUNDLE (HB); ANTI-HIV; ANTI-HIV ACTIVITY; CLINICAL APPLICATION; GP41; HELICAL CONTENT; HIV FUSION INHIBITORS; HIV INFECTION; INCLUSION BODIES; INHIBITORY ACTIVITY; LEUCINE SCANNING MUTAGENESIS; PROTEINASE K; SOLUBLE EXPRESSION; SOLUBLE STATE;

AMINO ACIDS; BACTERIA; BACTERIOLOGY; ESCHERICHIA COLI; MUTAGENESIS;

PEPTIDES;

BACTERIAL PROTEIN; C PEPTIDE; GLUTAMINE; HUMAN IMMUNODEFICIENCY VIRUS FUSION INHIBITOR; LEUCINE; PEPTIDE C52L; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; DISEASE TRANSMISSION; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN MODIFICATION; VIRUS STRAIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; C-PEPTIDE; ESCHERICHIA COLI; GENE EXPRESSION; HIV FUSION INHIBITORS; HIV INFECTIONS; HIV-1; HUMANS; LEUCINE; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SOLUBILITY;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 79959732694     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr013     Document Type: Article

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