Up-Regulation of Lipoxygenase, Phospholipase, and Oxylipin-Production in the Induced Chemical Defense of the Red Alga Gracilaria chilensis against Epiphytes

Journal of Chemical Ecology

Volumn 37, Issue 7, 2011, Pages 677-686

  Weinberger, Florian ^a,b,c   Lion, Ulrich ^d,e   Delage, Ludovic ^a,b   Kloareg, Bernard ^a,b   Potin, Philippe ^a,b   Beltrán, Jessica ^f   Flores, Verónica ^f   Faugeron, Sylvain ^f   Correa, Juan ^f   Pohnert, Georg ^d,e  

^a STATION BIOLOGIQUE DE ROSCOFF   (France)

^b SORBONNE UNIVERSITÉ   (France)

^c GEOMAR HELMHOLTZ CENTRE FOR OCEAN RESEARCH KIEL   (Germany)

^d FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^e MAX PLANCK INSTITUTE FOR CHEMICAL ECOLOGY   (Germany)

^f PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

Bioassays; Epiphytism; Gracilaria chilensis; Hormones; Induced defence; Red algae

Indexed keywords

CYCLOPENTANE DERIVATIVE; JASMONIC ACID; LIPOXYGENASE; OXYLIPIN; PHOSPHOLIPASE A; PHYTOHORMONE; PROSTAGLANDIN E2;

ALGAL CULTURE; BIOASSAY; CHEMICAL DEFENSE; COLLOID; ENZYME ACTIVITY; EPIPHYTE; HORMONE; METABOLITE; NATURAL ENEMY; POLYMERASE CHAIN REACTION; PURIFICATION; REAL TIME; RED ALGA; SEAWEED; SPORE;

ARTICLE; ENZYMOLOGY; GENETICS; GRACILARIA; METABOLISM; PHYSIOLOGY; PLANT IMMUNITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UPREGULATION;

CYCLOPENTANES; DINOPROSTONE; GRACILARIA; LIPOXYGENASE; OXYLIPINS; PHOSPHOLIPASES A; PLANT GROWTH REGULATORS; PLANT IMMUNITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; UP-REGULATION;

ALGAE; BACTERIA (MICROORGANISMS); GRACILARIA; GRACILARIA CHILENSIS; RHODOPHYTA;

EID: 79959661803     PISSN: 00980331     EISSN: 15731561     Source Type: Journal    
DOI: 10.1007/s10886-011-9981-9     Document Type: Article

References (42)

1. Asamizu E., Nakajima M., Kitade Y., Saga n., Nakamura Y., and Tabata S. 2003. Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis. J. Phycol. 39: 923-930.
2. Baldwin I. T. 1998. Jasmonate-induced responses are costly but benefit plants under attack in native populations. Proc. Natl. Acad. Sci. U. S. A. 95: 8113-8118.
3. Bonaventure G. and Baldwin I. T. 2010. New insights into the early biochemical activation of jasmonic acid biosynthesis in leaves. Plant Signal Behav 5: 287-9.
4. Borngraber S., Kuban R. J., Anton M., and Kuhn H. 1996. Phenylalanine 353 is a primary determinant for the positional specificity of mammalian 15-lipoxygenases. J. Mol. Biol. 264: 1145-1153.
5. Bouarab K., Adas F., Gaquerel E., Kloareg B., Salaun J. P., and Potin P. 2004. The innate immunity of a marine red alga involves oxylipins from both the eicosanoid and octadecanoid pathways. Plant Physiol. 135: 1838-1848.
6. Bradford M. M. 1976. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 72: 248-254.
7. Buschmann A. H., Correa J. A., Westermeier R., Hernandez-Gonzalez M. D., and Norambuena R. 2001. Red algal farming in Chile: A review. Aquaculture 194: 203-220.
8. Coleman R. A., Ramchunder S. J., Moody A. J., and Foggo A. 2007. An enzyme in snail saliva induces herbivore-resistance in a marine alga. Funct. Ecol. 21: 101-106.
9. Conrath U., Beckers G. J. M., Flors V., Garcia-Agustin P., Jakab G., Mauch F., Newman M. A., Pieterse C. M. J., Poinssot B., Pozo M. J., Pugin A., Schaffrath U., Ton J., Wendehenne D., Zimmerli L., and Mauch-Mani B. 2006. Priming: Getting ready for battle. Mol. Plant-Microbe Interact. 19: 1062-1071.
10. Correa J. A and McLachlan J. L. 1991. Endophytic algae of Chondrus crispus (Rhodophyta).3. Host specificity. J. Phycol. 27: 448-459.
11. De Vos M., van Oosten V. R., van Poecke R. M. P., van Pelt J. A., Pozo M. J., Mueller M. J., Buchala A. J., Metraux J. P., van Loon L. C., Dicke M., and Pieterse C. M. J. 2005. Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol. Plant-Microbe Interact. 18: 923-937.
12. Delumen B. O. and KazeniaC S. J. 1976. Staining for lipoxygenase activity in electrophoretic gels. Anal. Biochem. 72: 428-432.
13. Devoto A. and Turner J. G. 2005. Jasmonate-regulated Arabidopsis stress signalling network. Physiol. Plant 123: 161-172.
14. Farmer E. E. and Ryan C. A. 1990. Interplant communication-airborne methyl jasmonate induces synthesis of proteinase-inhibitors in plant-leaves. Proc. Natl. Acad. Sci. U. S. A. 87: 7713-7716.
15. Fusetani N. and Hashimoto K. 1984. Prostaglandin-E2-a candidate for causative agent of ogonori poisoning. Bulletin of the Japanese Society of Scientific Fisheries 50: 465-469.
16. Gaquerel E., Herve C., Labriere C., Boyen C., Potin P., and Salaun J. P. 2007. Evidence for oxylipin synthesis and induction of a new polyunsaturated fatty acid hydroxylase activity in Chondrus crispus in response to methyljasmonate. Biochim. Biophys. Acta, Mol. Cell Biol. Lipids 1771: 565-575.
17. Halitschke R., Kessler A., Kahl J., Lorenz A., and Baldwin I. T. 2000. Ecophysiological comparison of direct and indirect defenses in Nicotiana attenuata. Oecologia 124: 408-417.
18. Howe G. A. and Jander G. 2008. Plant immunity to insect herbivores. Annu. Rev. Plant Biol. 59: 41-66.
19. Kallenbach M., Alagna F., Baldwin I. T., and Bonaventure G. 2010. Nicotiana attenuata SIPK, WIPK, NPR1, and fatty acid-amino acid conjugates participate in the induction of jasmonic acid biosynthesis by affecting early enzymatic steps in the pathway. Plant Physiol. 152: 96-106.
20. Leonardi P. I., Miravalles A. B., Faugeron S., Flores V., Beltran J., and Correa J. A. 2006. Diversity, phenomenology and epidemiology of epiphytism in farmed Gracilaria chilensis (Rhodophyta) in northern Chile. Eur. J. Phycol.
21. Lion U., Wiesemeier T., Weinberger F., Beltran J., Flores V., Faugeron S., Correa J., and Pohnert G. 2006. Phospholipases and galactolipases trigger oxylipin-mediated wound-activated defence in the red alga Gracilaria chilensis against epiphytes. ChemBioChem 7: 457-462.
22. Liu Q. Y., and Reith M. E. 1994. Isolation of a gametophyte-specific cDNA encoding a lipoxygenase from the red alga Porphyra purpurea. Mol. Mar. Biol. Biotech. 3: 206-209.
23. Manchenko G. P. 1994. Handbook of Detection of Enzymes on Electrophoretic Gels.: CRC press, Boca Raton, USA.
24. Marchler-Bauer A., Anderson J. B., Cherukuri P. F., Dewweese-Scott C., Geer L. Y., Gwadz M., He S. Q., Hurwitz D. I., Jackson J. D., Ke Z. X., Lanczycki C. J., Liebert C. A., Liu C. L., Lu F., Marchler G. H., Mullokandov M., Shoemaker B. A., Simonyan V., Song J. S., Thiessen P. A., Yamashita R. A., Yin J. J., Zhang D. C., and Bryant S. H. 2005. CDD: a conserved domain database for protein classification. Nucleic Acids Res. 33: D192-D196.
25. Molis M., Korner J., Ko Y. W., Kim J. H., and Wahl M. 2006. Inducible responses in the brown seaweed Ecklonia cava: the role of grazer identity and season. J. Ecol. 94: 243-249.
26. Molis M., Korner J., Ko Y. W., and Kim J. H. 2008. Specificity of inducible seaweed anti-herbivory defences depends on identity of macroalgae and herbivores. Mar. Ecol. Prog. Ser. 354: 97-105.
27. Nylund G. M. and Pavia H. 2005. Chemical versus mechanical inhibition of fouling in the red alga Dilsea carnosa. Mar. Ecol. Prog. Ser. 299: 111-121.
28. Nylund G. M., Persson F., Lindegarth M., Cervin G., Hermansson M., and Pavia H. 2010. The red alga Bonnemaisonia asparagoides regulates epiphytic bacterial abundance and community composition by chemical defence. FEMS Microbiol. Ecol. 71: 84-93.
29. Paul N. A., De Nys R., and Steinberg P. D. 2006. Chemical defence against bacteria in the red alga Asparagopsis armata: Linking structure with function. Mar. Ecol. Prog. Ser. 306: 87-101.
30. Pavia H. and Toth G. B. 2000. Inducible chemical resistance to herbivory in the brown seaweed Ascophyllum nodosum. Ecology 81: 3212-3225.
31. Pohnert G. 2004. Chemical defense strategies of marine organisms. Top. Curr. Chem. 239: 179-219.
32. Potin P., Bouarab K., Salaun J. P., Pohnert G., and Kloareg B. 2002. Biotic interactions of marine algae. Curr. Opin. Plant Biol. 5: 308-317.
33. Prigge S. T., Boyington J. C., Faig M., Doctor K. S., Gaffney B. J., and Amzel L. M. 1997. Structure and mechanism of lipoxygenases. Biochimie 79: 629-636.
34. Rohde S. and Wahl M. 2008. Antifeeding defense in baltic macroalgae: Induction by direct grazing versus waterborne cues. J. Phycol. 44: 85-90.
35. Roy S., Pouenat M. L., Caumont C., Cariven C., PrevosT M. C., and Esquerretugaye M. T. 1995. Phospholipase acitivty and phospholipid patterns in tobacco cells treated with fungal elicitor. Plant Sci. (Amsterdam, Neth.) 107: 17-25.
36. Schuler G., Mithofer A., Baldwin I. T., Berger S., Ebel J., Santos J. G., Herrmann G., Holscher D., Kramell R., Kutchan T. M., Maucher H., Schneider B., Stenzel I., Wasternack C., and Boland W. 2004. Coronalon: a powerful tool in plant stress physiology. FEBS Lett. 563: 17-22.
37. Sloane D. L., Leung R., Craik C. S., and Sigal E. 1991. A primary determinant for lipoxygenase positional specificity. Nature 354: 149-152.
38. Toth G. B. and Pavia H. 2000. Water-borne cues induce chemical defense in a marine alga (Ascophyllum nodosum). Proc. Natl. Acad. Sci. U. S. A. 97: 14418-14420.
39. Truman W., Bennettt M. H., Kubigsteltig I., Turnbull C., and Grant M. 2007. Arabidopsis systemic immunity uses conserved defense signaling pathways and is mediated by jasmonates. Proc. Natl. Acad. Sci. U. S. A. 104: 1075-1080.
40. Weinberger F. 2007. Pathogen-induced defense and innate immunity in macroalgae. Biological Bulletin 213: 290-302.
41. Weinberger F., Pohnert G., Kloareg B., and Potin P. 2002. A signal released by an enclophytic attacker acts as a substrate for a rapid defensive reaction of the red alga Chondrus crispus. ChemBioChem 3: 1260-1263.
42. Wikstrom S. A. and Pavia H. 2004. Chemical settlement inhibition versus post-settlement mortality as an explanation for differential fouling of two congeneric seaweeds. Oecologia 138: 223-230.