Laser scanning cytometry and its applications: A pioneering technology in the field of quantitative imaging cytometry

Methods in Cell Biology

Volumn 102, Issue , 2011, Pages 159-205

  Henriksen, Melvin ^a   Miller, Bruce ^a   Newmark, Judith ^a   Al Kofahi, Yousef ^a   Holden, Elena ^a  

^a CompuCyte Corporation ^*  (United States)

Author keywords

Contouring; Data acquisition; Instrumentation; Laser scanning cytometry; Multiplexing; Thresholding

Indexed keywords

FLUORESCENT DYE; MEMBRANE ANTIGEN;

ANIMAL; APOPTOSIS; CELL AGING; CELL CYCLE; CHEMISTRY; DNA DAMAGE; HUMAN; IMMUNOPHENOTYPING; INSTRUMENTATION; LASER SCANNING CYTOMETRY; METHODOLOGY; REVIEW; SINGLE CELL ANALYSIS; TISSUE MICROARRAY;

ANIMALS; ANTIGENS, SURFACE; APOPTOSIS; CELL AGING; CELL CYCLE; DNA DAMAGE; FLUORESCENT DYES; HUMANS; IMMUNOPHENOTYPING; LASER SCANNING CYTOMETRY; SINGLE-CELL ANALYSIS; TISSUE ARRAY ANALYSIS;

EID: 79959608343     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-374912-3.00007-9     Document Type: Book

References (89)

1. Al-Za'abi A.M., Geddie W.B., et al. Equivalence of laser scanning cytometric and flow cytometric immunophenotyping of lymphoid lesions in cytologic samples. Am. J. Clin. Pathol. 2008, 129(5):780-785.
2. Albino A.P., Jorgenson E.D., Rainey P., et al. H2AX: A potential DNA damage response biomarker for assessing toxicological risk of tobacco products. Mutat. Res. 2009, 678:43-52.
3. Ayllon V., O'Connor R. PBK//TOPK promotes tumour cell proliferation through p38 MAPK activity and regulation of the DNA damage response. Oncogene 2006, 26(24):3451-3461.
4. Basiji, D.A., Ortyn, W.E., Liang, L., et al. (2007). Cellular image analysis and imaging by flow cytometry. In " Clin Lab Med." Vol. 27(3), pp. 653-viii. Amnis Corporation, Seattle, Washington.
5. Camara O., Rengsberger M., Egbe A., et al. The relevance of circulating epithelial tumor cells (CETC) for therapy monitoring during neoadjuvant (primary systemic) chemotherapy in breast cancer. Ann. Oncol. 2007, 18:1484-1492.
6. Chakraborty A.A., Tansey W.P. Inference of cell cycle-dependent proteolysis by laser scanning cytometry. Exp. Cell Res. 2009, 315(10):1772-1778.
7. Clatch R.J. Immunophenotyping of hematological malignancies by laser scanning cytometry. Methods Cell Biol. 2001, Vol. 64:313-342. Elsevier Inc, San Diego, CA.
8. Coons A.H., Kaplan M.M. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody. J. Exper. Med. 1950, 91:1-13.
9. Coulter W.H. High speed automatic blood cell counter and cell size analyzer. Proc. Natl. Electron. Conf. 1956, 12:1034.
10. Darzynkiewicz Z., Halicka D., Zhao H. Analysis of cellular DNA content by flow and laser scanning cytometry. Polyploidization in Cancer 2010, Landes Bioscience, Austin, TX. R. Poon (Ed.).
11. Darzynkiewicz Z., Pozarowski P., et al. Fluorochrome-labeled inhibitors of caspases: convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis. DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, Methods in Molecular Biology 2010, Vol. 682. Springer Science, Berlin.
12. Darzynkiewicz Z., Zhao H. Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry. DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, Methods in Molecular Biology 2010, Vol. 682. Springer Science, Berlin.
13. Darzynkiewicz Z., Smolewski P., et al. Laser scanning cytometry for automation of the micronucleus assay. Mutagenesis 2011, 26(1):153-161.
14. Dmitrieva N.I. Analysis of DNA breaks, DNA damage response, and apoptosis produced by high NaCl. Am. J. Physiol. Renal Physiol. 2008, 295:F1678-F1688.
15. Faiola B., Falls J.G., Peterson R.A., et al. PPAR alpha more than PPAR delta, mediates the hepatic and skeletal muscle alterations induced by the PPAR agonist GW0742. Toxicol. Sci. 2008, 105:384-394.
16. Friedman B., Shachtrup C., Tsai P.S., et al. Acute vascular disruption and aquaporin 4 loss after stroke. Stroke 2009, 40(6):2182-2190.
17. Fulwyler M.J. Electronic separation of biological cells by volume. Science 1965, 150:910.
18. Geddie William R. Cytology and laser scanning cytometry. Wintrobe's Atlas of Clinical Hematology 2007, Lippincott Williams & Wilkins, Baltimore, MD. D. Tkachuk, J.V. Hirschmann (Eds.).
19. Ghavami S., et al. Statin-triggered cell death in primary human lung mesenchymal cells involves p53-PUMA and release of Smac and Omi but not cytochrome c. Biochem. Biophys. Acta 2010, 1803(4):452-467.
20. Goodale D., Phay C., Postenka C., et al. Characterization of tumor cell dissemination patterns in preclinical models of cancer metastasis using flow cytometry and laser scanning cytometry. Cytometry A 2008, 75(4):344-355.
21. Hamamoto T., Suzuki K., Kodama H., et al. Correlation of malignant phenotypes of human tumour cell lines with augmented expression of Hsp72 protein measured by laser scanning cytometry. Int. J. Hyperthermia 2007, 23(4):363-370.
22. Harnett M. Laser scanning cytometry: Understanding the immune system in situ. Nature Rev. Immunol. 2007, 7:897-904.
23. Hjelmeland L.M., Fujikawa A., et al. Quantification of retinal pigment epithelial phenotypic variation using laser scanning cytometry. Mol. Vision 2010, 16:1108-1121.
24. Holme A.L., Yadav S., Pervaiz S. Automated laser scanning cytometry: a powerful tool for multi-parameter analysis of drug-induced apoptosis. Cytometry A 2007, 71(2):80-86.
25. Iglesias I., Bentsi-Barnes K., Umeadi C., et al. Comprehensive analysis of human pancreatic islets using flow and laser scanning cytometry. Transplant. Proc. 2008, 40(2):351-354.
26. Ichii H., Miki A., Yamamoto T., et al. Characterization of pancreatic ductal cells in human islet preparations. Lab Invest. 2008, 88(11):1167-1177.
27. Ichii H., Sakuma Y., Pileggi A., et al. Shipment of human islets for transplantation. Am. J. Transplant. 2007, 7(4):1010-1020.
28. Ito T., Omoro K., Rawson J., et al. Improvement of canine islet yield by donor pancreas infusion with a p38MAPK inhibitor. Transplantation 2008, 86:321-329.
29. Jacobberger J.W., Frisa P., Sramkoski R., et al. A new biomarker for mitotic cells. Cytometry A 2008, 73(1):5-15.
30. Jorgensen E.D., Zhao H., et al. DNA damage response induced by exposure of human lung adenocarcinoma cells to smoke from tobacco- and nicotine-free cigarettes. Cell Cycle 2010, 9(11):2170-2176.
31. Jul-Larsen A., Grudic A., Bjerkvig R., Ove Boe R. Cell-cycle regulation and dynamics of cytoplasmic compartments containing the promyelocytic leukemia protein and nucleoporins. J. Cell Sci. 2009, 122:1201-1210.
32. Kamentsky, L. A. (2001). Laser scanning cytometry. In " Methods in Cell Biology" 63, 51-87.
33. Kamentsky L.A., Derman H., Melamed M.R. Ultraviolet Absorption in Epidermoid Cancer Cells. Science 1963, 142:1580-1583.
34. Kamentsky L.A., Kamentsky L.D. Microscope-based multiparameter laser scanning cytometer yielding data comparable to flow cytometry data. Cytometry 1991, 12:381-387.
35. Kamentsky L.A., Melamed M.R. Spectrophotometric cell sorter. Science 1967, 156:1364-1365.
36. Kato M., Putta S., Wang M., et al. TGF-B activates Akt kinase through a micro RNA-dependent amplifying circuit targeting PTEN. Nature Cell Biol. 2009, 11(7):881-889.
37. Kawamura K., et al. Induction of Centrosome Amplification and Chromosome Instability in Human Bladder Cancer Cells by p53 Mutation and Cyclin E Overexpression. Cancer Res. 2004, 64:4800-4809.
38. Kawauchi S., Furiya T., et al. DNA copy number aberrations associated with aneuploidy and chromosomal instability in breast cancers. Oncol. Rep. 2010, 24(4):875-883.
39. Kuliffay P., Sanislo L., Galbavy S. Chromatin texture, DNA index, and S-phase fraction in primary breast carcinoma cells analysed by laser scanning cytometry. Bratis Lek Listy 2010, 111(1):4-8.
40. Kunos C., Radivoyevitch T., Pink J., et al. Ribonucleotide reductase inhibition enhances chemoradiosensitivity of human cervical cancers. Radiat. Res. 2010, 174(5):574-581.
41. Li X., Darzynkiewicz Z. The Schrödinger's cat quandary in cell biology: integration of live cell functional assays with measurements of fixed cells in analysis of apoptosis. Exp. Cell Res. 1999, 249(2):404-412.
42. Lodish Harvey F. Molecular Cell Biology 2008, St. Martin's Press, New York, p. 387.
43. Luther E., Geddie W. Laser scatter in clinical applications. Proc. SPIE 2008, 6864. 686412; DOI:10.1117/12.763996, Online Publication Date: 15 February 2008.
44. Luther E., Kamentsky L.A. Resolution of mitotic cells using laser scanning cytometry. Cytometry 1996, 23:272-278.
45. Luther E., Kamentsky L., et al. Next-generation laser scanning cytometry. Methods in Cell Biology 2004, 75:185-218. Academic Press, New York.
46. MacDonald, K., Palmer, J., et al. (2010). An antibody against the colony-stimulating factor 1 receptor (CSF1R). depletes the resident subset of monocytes and tissue and tumor-associated macrophages but does not inhibit inflammation. Blood prepublished online August 3, 2010; DOI:10.1182/blood 2010-02-266296.
47. Mei Y., Saha K., et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nature Mater. 2010, 9:768-778.
48. Mei Ying, et al. Mapping the interactions among biomaterials, adsorbed proteins, and human embryonic stem cells. Adv. Mater. 2009, 21:1-6.
49. Minsky M. Memoir on inventing the confocal scanning microscope. Scanning 1988, 10:128-138.
50. Miranda-Carboni G.A., Krum S., Yee K., et al. A functional link between Wnt signaling and SKP2-independent p27 turnover in mammary tumors. Genes Dev. 2008, 22:31221-31234.
51. Mora J., Lavarino C., et al. Comprehensive analysis of tumoral DNA content reveals clonal ploidy heterogeneity as a marker with prognostic significance in locoregional neuroblastoma. Genes Chromosomes Cancer 2007, 46(4):385-396.
52. Nombela-Arrieta C., et al. Spatial analysis of hematopoietic stem and progenitor cells in the bone marrow. Blood (ASH Annual Meeting Abstract) 2008, 112:3570.
53. Olive K.P., et al. Inhibition of hedgehog signaling enhances delivery of chemotherapy in a mouse model of pancreatic cancer. Science 2009, 10:1126.
54. Omori K., Valiente K., et al. Improvement of Human Islet Cryopreservation by a p38 MAPK Inhibitor. Am. J. Transplantation 2007, 7(5):1224-1232.
55. Omori K., Mistuhashi M., et al. Microassay for glucose-induced preproinsulin mRNA expression to assess islet transplantation. Transplantation 2010, 89(2):146-154.
56. Pachmann K., Camara O., et al. Monitoring the response of circulating epithelial tumor cells to adjuvant chemotherapy in breast cancer allows detection of patients at risk of early relapse. J. Clin. Oncol. 2008, 26(8):1208-1215.
57. Pawley, James B. Handbook of Biological Confocal Microscopy 2006, Springer, New York, pp. 4, 223ff.
58. Peterson R.A., Krull D., et al. Applications of laser scanning cytometry in immunohistochemistry and routine histopathology. Toxicol. Pathol. 2008, 36(1):117-132.
59. Ploem J.S. The use of a vertical illuminator with interchangeable dielectric mirrors for fluorescence microscopy with incident light. Z. Wiss. Mikrosk. 1967, 68:129-142.
60. Ploem J.S. Fluorescence microscopy. Fluorescent and Luminescent Probes for Biological Activity 1993, 1-11. Academic Press, London. W.T. Mason (Ed.).
61. Pozarowski P., Holden E., Darzynkiewicz Z. Laser scanning cytometry: principles and applications. Methods in Molecular Biology 2006, Vol. 319:165-192. Humana Press, Clifton, NJ.
62. Rieber M.S., Viola-Rhenals M., Rieber M. Attenuation of genotoxicity under adhesion-restrictive conditions through modulation of p53, γ H2AX and nuclear DNA organization. Apoptosis 2007, 12:449-458.
63. Rieber M.S., Viola-Rhenals M., Rieber M. Role of peroxidases, thiols and Bak/Bax in tumor cell susceptibility to Cu[DEDTC]2. Biochem. Pharmacol. 2007, 74:841-850.
64. Sakaguchi A., Steward R. Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster. J. Cell Biol. 2007, 176:155-162.
65. Sanoslo L., Kuliffay P., et al. Advanced detection and measurement of cells on membrane from peripheral blood by laser scanning cytometry (LSC) in early stage breast cancer patients. Bratisl Lek Listy 2010, 111(1):13-19.
66. Schwock J., Ho J.C., Luther E., et al. Measurement of signaling pathway activities in solid tumor fine-needle biopsies by slide-based cytometry. Diagn. Molec. Pathol. 2007, 16(3):130-140.
67. Shapiro H.M. Practical Flow Cytometry 2003, Wiley-Liss, New York. Fourth ed.
68. Stegh A.H., Kesari S., et al. Bcl2L12-mediated inhibition of effector caspase 3 and 7 via distinct mechanisms in gliobastoma. Proc. Natl. Acad. Sci. U.S.A. 2008, 105(31):10703-10708.
69. Swanberg S.E., Nagarajan R.P., Peddada S., et al. Reciprocal co-regulation of EGR2 and MECP2 is disrupted in Rett syndrome and autism. Human Mol. Gen. 2009, 18(3):525-534.
70. Sweet I.R., Gilbert M., et al. Glucose-stimulated increment in oxygen consumption rate as a standardized test of human islet quality. Am. J. Transplant. 2008, 8:183-192.
71. Takahashi H., Ruiz P., Ricordi C., Miki A., et al. In situ quantitative immunoprofiling of regulatory T cells using laser scanning cytometry. Transplant. Proc. 2009, 41(1):238-239.
72. Tanaka T., Halicka D., Traganos F., Darzynkiewicz Z. Cytometric analysis of DNA damage: phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs). Methods in Molecular Biology 2009, 523:161-168.
73. Tanaka T., Huang X., et al. Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A 2007, 71(9):648-661.
74. Tanaka T., Huang X., et al. ATM activation accompanies histone H2AX phosphorylation in A529 cells upon exposure to tobacco smoke. BMC Cell Biol. 2007, 8:1-11. 26.
75. Tellez C.S., Davis D.W., et al. Quantitative analysis of melanocytic tissue array reveals inverse correlation between activator protein-2alpha and protease-activated receptor-1 expression during melanoma progression. J. Invest. Dermatol. 2007, 127(2):387-393.
76. Todorov I., et al. Quantitative assessment of β-cell apoptosis and cell composition of isolated, undisrupted human islets by laser scanning cytometry. Transplantation 2010, 90(8):836-842.
77. Tsujioka T., Tochigi A., et al. DNA ploidy and cell cycle analyses in the bone marrow cells of patients with megaloblastic anemia using laser scanning cytometry. Cytometry B Clin. Cytom. 2008, 74(2):104-109.
78. Van Dilla M.A., Trujillo T.T., Mullaney P.F., Coulter J.R. Cell microfluorometry: a method for rapid fluorescence measurement. Science 1969, 163:1213-1214.
79. Vincent L., Soille P. Watersheds in digital spaces: an efficient algorithm based on immersion simulations. IEEE Trans. Pattern Anal. Mach. Intell. 1991, 13(6):583-598.
80. Williams Kirsten M., et al. Single cell analysis of complex thymus stromal cell populations: rapid thymic epithelia preparation characterizes radiation injury. Clin. Transl. Sci. 2009, 2(4):279-285.
81. Zhao Hong, et al. Induction of DNA damage response by the supravital probes of nucleic acids. Cytometry A 2009, 75(6):510-519.
82. Zhao H., Halicka D., Traganos F., Jorgensen E., Darzynkiewicz Z. New biomarkers probing depth of cell senescence assessed by laser scanning cytometry. Cytometry A 2010, 77(11):999-1007.
83. Zhao H., et al. The cytotoxic ribonuclease onconase targets RNA interference (siRNA). Cell Cycle 2008, 7(20):3258-3261.
84. Zhao H., Traganos F., Darzynkiewicz Z. Kinetics of histone H2AX phosphorylation and Chk2aActivation in A549 cells treated with topotecan and mitozantrone in relation to cell cycle phase. Cytometry A 2008, 73:480-489.
85. Zhao H., Traganos F., Darzynkiewicz Z. Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication. Cytometry A 2010, 77(3):285-293.
86. Zhao H., Tanaka T., et al. Cytometric assessment of DNA damage by exogenous and endogenous oxidants reports aging-related processes. Cytometry A 2007, 71A:905-914.
87. Zheng X.L., Yuan S.G., et al. Phenotype-specific inhibition of the vascular smooth muscle cell cycle by high glucose treatment. Diabetologia 2007, 50(4):881-890.
88. Zoog S.J., et al. Measurement of conatumumab-induced apoptotic activity in tumors by fine needle aspirate sampling. Cytometry A 2010, 77A:849-860.
89. Zoog S.J., et al. Antagonists of CD117 (cKit). Signaling inhibit mast cell accumulation in healing skin wounds. Cytometry A 2008, 75(3):189-198.