Lentivirus-mediated inhibition of Med19 suppresses growth of breast cancer cells in vitro

Cancer Chemotherapy and Pharmacology

Volumn 68, Issue 1, 2011, Pages 207-215

  Li, Li Hua ^a,b   He, Jie ^a   Hua, Dong ^a   Guo, Zi Jian ^a   Gao, Qi ^b,c  

^a SOOCHOW UNIVERSITY   (China)

^b MEDICAL COLLEGE OF SOOCHOW UNIVERSITY   (China)

^c JIANGSU INSTITUTE OF PARASITIC DISEASES   (China)

Author keywords

Breast cancer; Lentivirus; Med19; RNA interference

Indexed keywords

BROXURIDINE; LENTIVIRUS VECTOR; MULTIPROTEIN COMPLEX; PROTEIN MED19; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BREAST CANCER; CANCER CELL; CANCER GRADING; CANCER INHIBITION; CANCER TISSUE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL CYCLE REGULATION; CELL PROLIFERATION; CELL STRAIN; CELL STRAIN MCF 7; CELL STRAIN MDA MB 231; CLINICAL ARTICLE; COLONY FORMATION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LENTIVIRINAE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; UPREGULATION; VIRAL GENE DELIVERY SYSTEM;

BREAST NEOPLASMS; CELL LINE, TUMOR; CELL PROLIFERATION; FEMALE; G1 PHASE; HUMANS; LENTIVIRUS; MEDIATOR COMPLEX; PLASMIDS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSCRIPTION, GENETIC; UP-REGULATION;

EID: 79959601916     PISSN: 03445704     EISSN: 14320843     Source Type: Journal    
DOI: 10.1007/s00280-010-1468-9     Document Type: Article

References (28)

1. Boube M, Joulia L, Cribbs DL, Bourbon HM (2002) Evidence for a mediator of RNA polymerase II transcriptional regulation conserved from yeast to man. Cell 110:143-151 (Pubitemid 34876543)
2. Rosenblum-Vos LS, Rhodes L, Evangelista CC Jr, Boayke KA, Zitomer RS (1991) The ROX3 gene encodes an essential nuclear protein involved in CYC7 gene expression in Saccharomyces cerevisiae. Mol Cell Biol 11:5639-5647 (Pubitemid 21895331)
3. Gustafsson CM, Myers LC, Li Y, Redd MJ, Lui M, Erdjument-Bromage H, Tempst P, Kornberg RD (1997) Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme. J Biol Chem 272:48-50 (Pubitemid 27021123)
4. Chadick JZ, Asturias FJ (2005) Structure of eukaryotic Mediator complexes. Trends Biochem Sci 30:264-271 (Pubitemid 40693870)
5. Beve J, Hu GZ, Myers LC, Balciunas D, Werngren O, Hultenby K, Wibom R, Ronne H, Gustafsson CM (2005) The structural and functional role of Med5 in the yeast Mediator tail module. J Biol Chem 280:41366-41372 (Pubitemid 41832194)
6. Singh H, Erkine AM, Kremer SB, Duttweiler HM, Davis DA, Iqbal J, Gross RR, Gross DS (2006) A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression. Genetics 172:2169-2184
7. Baidoobonso SM, Guidi BW, Myers LC (2007) Med19 (Rox3) regulates Intermodule interactions in the Saccharomyces cerevisiae mediator complex. J Biol Chem 282:5551-5559 (Pubitemid 47093718)
8. Ding N, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Boyer TG (2009) MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression. J Biol Chem 284:2648-2656
9. Castanotto D, Rossi JJ (2009) The promises and pitfalls of RNA-interference-based therapeutics. Nature 457:426-433
10. Haasnoot J, Westerhout EM, Berkhout B (2007) RNA interference against viruses: strike and counterstrike. Nat Biotechnol 25:1435-1443 (Pubitemid 350233142)
11. Li CI, Daling JR, Malone KE (2005) Age-specific incidence rates of in situ breast carcinomas by histologic type, 1980 to 2001. Cancer Epidemiol Biomarkers Prev 14:1008-1011 (Pubitemid 40478573)
12. Allgayer H, Fulda S (2008) An introduction to molecular targeted therapy of cancer. Adv Med Sci 53:130-138
13. Nishitsuji H, Ikeda T, Miyoshi H, Ohashi T, Kannagi M, Masuda T (2004) Expression of small hairpin RNA by lentivirus-based vector confers efficient and stable gene-suppression of HIV-1 on human cells including primary non-dividing cells. Microbes Infect 6:76-85 (Pubitemid 38114824)
14. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Zhang M, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L (2003) A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 33:401-406 (Pubitemid 36278858)
15. Pullmann R Jr, Juhaszova M, Lopez de Silanes I, Kawai T, Mazan-Mamczarz K, Halushka MK, Gorospe M (2005) Enhanced proliferation of cultured human vascular smooth muscle cells linked to increased function of RNA-binding protein HuR. J Biol Chem 280:22819-22826 (Pubitemid 40853188)
16. Zielske SP, Stevenson M (2005) Importin 7 may be dispensable for human immunodeficiency virus type 1 and simian immunodeficiency virus infection of primary macrophages. J Virol 79:11541-11546 (Pubitemid 41170699)
17. Sakoda T, Kasahara N, Hamamori Y, Kedes L (1999) A high-titer lentiviral production system mediates efficient transduction of differentiated cells including beating cardiac myocytes. J Mol Cell Cardiol 31:2037-2047 (Pubitemid 29537603)
18. Soneoka Y, Cannon PM, Ramsdale EE, Griffiths JC, Romano G, Kingsman SM, Kingsman AJ (1995) A transient three-plasmid expression system for the production of high titer retroviral vectors. Nucleic Acids Res 23:628-633
19. Tiscornia G, Singer O, Verma IM (2006) Production and purification of lentiviral vectors. Nat Protoc 1:241-245
20. Pfaffl MW, Horgan GW, Dempfle L (2002) Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 30:e36
21. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553 (Pubitemid 34408678)
22. Xia H, Mao Q, Paulson HL, Davidson BL (2002) siRNA-mediated gene silencing in vitro and in vivo. Nat Biotechnol 20:1006-1010
23. Brummelkamp TR, Bernards R, Agami R (2002) Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2:243-247 (Pubitemid 41043979)
24. Qin XF, An DS, Chen IS, Baltimore D (2003) Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc Natl Acad Sci USA 100:183-188
25. ter Brake O, Konstantinova P, Ceylan M, Berkhout B (2006) Silencing of HIV-1 with RNA interference: a multiple shRNA approach. Mol Ther 14:883-892
26. Manjunath N, Wu H, Subramanya S, Shankar P (2009) Lentiviral delivery of short hairpin RNAs. Adv Drug Deliv Rev 61:732-745
27. Bank A, Dorazio R, Leboulch P (2005) A phase I/II clinical trial of beta-globin gene therapy for beta-thalassemia. Ann N Y Acad Sci 1054:308-316 (Pubitemid 43031038)
28. Manilla P, Rebello T, Afable C, Lu X, Slepushkin V, Humeau LM, Schonely K, Ni Y, Binder GK, Levine BL, MacGregor RR, June CH, Dropulic B (2005) Regulatory considerations for novel gene therapy products: a review of the process leading to the first clinical lentiviral vector. Hum Gene Ther 16:17-25 (Pubitemid 40250328)