Effects of chitin binding domain on enzymatic properties and insecticidal activity of Bombyx mori chitinase

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 7, 2011, Pages 1551-1558

  Fan, Yanhua ^a   Guo, Shujun ^a   Pei, Xiaoqiong ^a   Zhang, Yongjun ^a   Luo, Zhibing ^a   Pei, Yan ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Bombyx mori; Chitin binding domain (ChBD); Chitinase; Ecdysis; Virulence

Indexed keywords

BOMBYX MORI; CHITIN BINDING DOMAIN (CHBD); CHITINASE; ECDYSIS; VIRULENCE;

AMINO ACIDS; CHITIN;

ANIMALS;

BASSIANA; BOMBYX MORI; FUNGI; HEXAPODA; PICHIA PASTORIS;

EID: 79959506362     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-010-0607-0     Document Type: Article

References (42)

1. Abdel-Banat BM, Koga D (2001) A genomic clone for a chitinase gene from the silkworm, Bombyx mori: structural organization identifies functional motifs. Insect Biochem Mol Biol 31: 491-508.
2. Abdel-Banat BM, Koga D (2002) Alternative splicing of the primary transcript generates heterogeneity within the products of the gene for Bombyx mori chitinase. J Biol Chem 277: 30524-30534.
3. Abdel-Banat BM, Banat YK, Kameyama Y, Yoshioka T, Koga D (1999) Purification and characterization of a 54 kDa chitinase from Bombyx mori. Insect Biochem Mol Biol 29: 537-547.
4. Arakane Y, Zhu Q, Matsumiya M, Muthukrishnan S, Kramer KJ (2003) Properties of catalytic, linker and chitin-binding domains of insect chitinase. Insect Biochem Mol Biol 33: 631-648.
5. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
6. Charnley AK (1997) Entomopathogenic fungi and their role in pest control. In: Wicklow D and Soderstrom M (ed) The mycota, environmental and microbial relationships, vol 4. Springer-Verlag, Heidelberg, pp 185-201.
7. Cho EM, Boucias D, Keyhani NO (2006) EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. II. Fungal cells sporulating on chitin and producing oosporein. Microbiology Sgm 152: 2855-2864.
8. Daimon T, Katsuma S, Iwanaga M, Kang W, Shimada T (2005) The Bmchi-h gene, a bacterial type chitinase gene of Bombyx mori, encodes a functional exochitinase that plays a role in the chitin degradation during the molting process. Insect Biochem Mol Biol 35: 1112-1123.
9. Ding X (1995) Manduca chitinase-mediated resistance to tabacco budworm (Heliothis virescens) and tabacco hornworm (Manduca sexta) larvae in transgenic tobacco plants. Dissertation, Kansas State University, Manhattan.
10. Ding X, Gopalakrishnan B, Johnson LB, White FF, Wang X, Morgan TD, Kramer KJ, Muthukrishnan S (1998) Insect resistance of transgenic tobacco expressing an insect chitinase gene. Transgenic Res 7: 77-84.
11. Fan Y, Fang W, Guo S, Pei X, Zhang Y, Xiao Y, Li D, Jin K, Bidochka MJ, Pei Y (2007a) Increased insect virulence in Beauveria bassiana overexpressing an engineered chitinase with the chitin binding domain from Bombyx mori. Appl Environ Microbiol 73: 295-302.
12. Fan Y, Zhang Y, Yang X, Pei X, Guo S, Pei Y (2007b) Expression of a Beauveria bassiana chitinase (Bbchit1) in Escherichia coli and Pichia pastoris. Protein Expr Purif 56: 93-99.
13. Fan Y, Pei X, Guo S, Zhang Y, Luo Z, Liao X, Pei Y (2010) Increased virulence using engineered protease-chitin binding domain hybrid expressed in the entomopathogenic fungus Beauveria bassiana. Microb Pathog 49: 376-380.
14. Fang W, Leng B, Xiao Y, Jin K, Ma J, Fan Y, Feng J, Yang X, Zhang Y, Pei Y (2005) Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 71: 363-370.
15. Fitches E, Wilkinson H, Bell H, Bown DP, Gatehouse JA, Edwards JP (2004) Cloning, expression and functional characterisation of chitinase from larvae of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase. Insect Biochem Mol Biol 34: 1037-1050.
16. Gopalakrishnan B, Muthukrishnan S, Kramer KJ (1995) Baculovirus-mediated expression of a Manduca sexta chitinase gene: properties of the recombinant protein. Insect Biochem Mol Biol 25: 255-265.
17. Henrissat B (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 280: 309-316.
18. Hepburn HR (1985) Structure of the integument. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry, and pharmacology, vol 3. Pergamon Press, Oxford, pp 1-58.
19. Holder DJ, Kirkland BH, Lewis MW, Keyhani NO (2007) Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana. Microbiology Sgm 153: 3448-3457.
20. Huang CJ, Chen CY (2005) High-level expression and characterization of two chitinases, ChiCH and ChiCW, of Bacillus cereus 28-9 in Escherichia coli. Biochem Biophys Res Commun 327: 8-17.
21. Ikegami T, Okada T, Hashimoto M, Seino S, Watanabe T, Shirakawa M (2000) Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J Biol Chem 275: 13654-13661.
22. Iseli B, Boller T, Neuhaus JM (1993) The N-terminal cysteine rich domain of tobacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity. Plant Physiol 103: 221-226.
23. Itoh Y, Kawase T, Nikaidou N, Fukada H, Mitsutomi M, Watanabe T, Itoh Y (2002) Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function. Biosci Biotechnol Biochem 66: 1084-1092.
24. Jang MK, Kong BG, Jeong YI, Lee CH, Nah JW (2004) Physicochemical characterization of α-chitin, β-chitin, and γ-chitin separated from natural resources. J Polym Sci Part A 42: 3423-3432.
25. Jin K, Zhang Y, Luo Z, Xiao Y, Fan Y, Wu D, Pei Y (2008) An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker. Biotechnol Lett 30: 1379-1383.
26. Kabir KE, Sugimoto H, Tado H, Endo K, Yamanaka A, Tanaka S, Koga D (2006) Effect of Bombyx mori chitinase against Japanese pine sawyer (Monochamus alternatus) adults as a biopesticide. Biosci Biotechnol Biochem 70: 219-229.
27. Kim MG, Shin SW, Bae KS, Kim SC, Park HY (1998) Molecular cloning of chitinase cDNAs from the silkworm, Bombyx mori and the fall webworm Hyphantria cunea. Insect Biochem Molec Biol 28: 163-171.
28. Koga D, Sasaki Y, Uchiumi Y, Hirai N, Arakane Y, Nagamatsu Y (1997) Purification and characterization of Bombyx mori chitinases. Insect Biochem Mol Biol 27: 757-767.
29. Kramer KJ, Muthukrishnan S (1997) Insect chitinases: molecular biology and potential use as biopesticides. Insect Biochem Mol Biol 27: 887-900.
30. Kramer KJ, Corpuz LM, Choi H, Muthukrishnan S (1993) Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta. Insect Biochem Molec Biol 23: 691-701.
31. Krishnan A, Nair PN, Jones D (1994) Isolation, cloning and characterization of a new chitinase stored in active form in chitinlined venom reservoir. J Biol Chem 269: 20971-20976.
32. Lewis MW, Robalino IV, Keyhani NO (2009) Uptake of the fluorescent probe FM4-64 by hyphae and haemolymph-derived in vivo hyphal bodies of the entomopathogenic fungus Beauveria bassiana. Microbiology 155(Pt 9): 3110-3120.
33. Lu D, Pava-Ripoll M, Li Z, Wang C (2008) Insecticidal evaluation of Beauveria bassiana engineered to express a scorpion neurotoxin and a cuticle degrading protease. Appl Microbiol Biotechnol 81: 515-522.
34. Merzendorfer H, Zimoch L (2003) Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol 206: 4393-4412.
35. Mikitani K, Sugasaki T, Shimada T, Kobayashi M, Gustafsson JA (2000) The chitinase gene of the silkworm, Bombyx mori, contains a novel Tc-like transposable element. J Biol Chem 275: 37725-37732.
36. Pedrini N, Zhang S, Juarez MP, Keyhani NO (2010) Molecular characterization and expression analysis of a suite of cytochrome P450 enzymes implicated in insect hydrocarbon degradation in the entomopathogenic fungus Beauveria bassiana. Microbiology doi: 10. 1099/mic. 0. 039735-0.
37. Reynolds SE, Samuels R (1996) Physiology and biochemistry of insect moulting fluid. Adv Insect Physiol 26: 157-232.
38. Songjang K, Donchai T, Chaiyawat P, Meyer C (2006) Cloning and expression of chitinase gene isolated from insect pathogenic fungi Beauveria bassiana in Escherichia coli. Chiang Mai J Sci 33: 347-355.
39. St. Leger RJ, Joshi L, Bidochka MJ, Roberts DW (1996) Construction of an improved mycoinsecticide overexpressing a toxic protease. Proc Natl Acad Sci USA 93: 6349-6354.
40. St Leger RJ, Wang C (2010) Genetic engineering of fungal biocontrol agents to achieve greater efficacy against insect pests. Appl Microbiol Biotechnol 85: 901-907.
41. Wanchoo A, Lewis MW, Keyhani NO (2009) Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana. Microbiology 155(Pt 9): 3121-3133.
42. Zhu X, Zhang H, Fukamizo T, Muthukrishnan S, Kramer KJ (2001) Properties of Manduca sexta chitinase and its C-terminal deletions. Insect Biochem Mol Biol 31: 1221-1230.