Increased virulence using engineered protease-chitin binding domain hybrid expressed in the entomopathogenic fungus beauveria bassiana

Microbial Pathogenesis

Volumn 49, Issue 6, 2010, Pages 376-380

  Fan, Yanhua ^a   Pei, Xiaoqiong ^a   Guo, Shujuan ^a   Zhang, Yongjun ^a   Luo, Zhibing ^a   Liao, Xinggang ^a   Pei, Yan ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CEDP 1 PROTEIN; CHITIN; CHITINASE; HYBRID PROTEIN; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BEAUVERIA BASSIANA; CONTROLLED STUDY; CUTICLE; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS GROWTH; FUNGUS HYPHAE; GENETIC ENGINEERING; INSECTICIDAL ACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN DOMAIN; SILKWORM; STRAIN DIFFERENCE; STRAIN IMPROVEMENT;

ANIMALS; BEAUVERIA; BINDING SITES; BOMBYX; CHITIN; CHITINASE; INSECT PROTEINS; INSECTS; PEPTIDE HYDROLASES; PEST CONTROL, BIOLOGICAL; PICHIA; RECOMBINANT FUSION PROTEINS; SURVIVAL ANALYSIS; VIRULENCE; VIRULENCE FACTORS;

BOMBYX MORI; CORDYCEPS BASSIANA; FUNGI; HEXAPODA; PICHIA PASTORIS;

EID: 77957773756     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2010.06.013     Document Type: Article

References (36)

1. Samson R.A., Evans C.S., Latge J.P. Atlas of entomopathogenic fungi 1988, Springer-Verlag, Berlin.
2. Lomer C.J., Bateman R.P., Johnson D.L., Langewald J., Thomas M. Biological control of locusts and grasshoppers. Annu Rev Entomol 2001, 46:667-702.
3. Reynolds S.E., Samuels R.I. Physiology and biochemistry of insect moulting fluid. Adv Insect Physiol 1996, 26:157-232.
4. Inglis G.D., Goettel M.S., Butt T.M., Strasser H. Use of hyphomycetous fungi for managing insect pests. Field manual of techniques in invertebrate pathology 2001, 651-679. Kluwer Academic, Netherlands. L.L.A. In, H.K. Kaya (Eds.).
5. Kirkland B.H., Westwood G.S., Keyhani N.O. Pathogenicity of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to Ixodidae tick species Dermacentor variabilis, Rhipicephalus sanguineus, and Ixodes scapularis. J Med Entomol 2004, 41:705-711.
6. Scholte E.J., Knols B.G.J., Samson R.A., Takken W. Entomopathogenic fungi for mosquito control: a review. J Insect Sci 2004, 4:1-24.
7. Dimbi S., Maniania N.K., Lux S.A., Ekesi S., Mueke J.K. Pathogenicity of Metarhizium anisopliae (Metsch) Sorokin and Beauveria bassiana (Balsamo) Vuillemin, to three adult fruit fly species: Ceratitis capitata (Weidemann), C. rosa var. fasciventris Karsch and C. cosyra (Walker) (Diptera:Tephritidae). Mycopathologia 2003, 156:375-382.
8. Lecuona R.E., Turica M., Tarocco F., Crespo D.C. Microbial control of Musca domestica (Diptera: Muscidae) with selected strains of Beauveria bassiana. J Med Entomol 2005, 42:332-336.
9. Kassa A., Stephan D., Vidal S., Zimmermann G. Production and processing of Metarhizium anisopliae var. acridum submerged conidia for locust and grasshopper control. Mycol Res 2004, 108:93-100.
10. McCoy C.W. Entomogenous fungi as microbial pestidides. New directions in biological control 1990, 139-159. A.R. Liss, New York, NY. R.R. Baker, P.E. Dunn (Eds.).
11. St.Leger R.J., Joshi L., Bidochka M.J., Roberts D.W. Construction of an improved mycoinsecticide overexpressing a toxic protease. Proc Natl Acad Sci USA 1996, 93:6349-6354.
12. Holder D.J., Keyhani N.O. Adhesion of the entomopathogenic fungus Beauveria (Cordyceps) bassiana to substrata. Appl Environ Microbiol 2005, 71:5260-5266.
13. Holder D.J., Kirkland B.H., Lewis M.W., Keyhani N.O. Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana. Microbiology-Sgm 2007, 153:3448-3457.
14. Freimoser F.M., Hu G., St.Leger R.J. Variation in gene expression patterns as the insect pathogen Metarhizium anisopliae adapts to different host cuticles or nutrient deprivation in vitro. Microbiology 2005, 151:361-371.
15. Fang W., Leng B., Xiao Y., Jin K., Ma J., Fan Y., et al. Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence. Appl Environ Microbiol 2005, 71:363-370.
16. Screen S.E., Hu G., St.Leger R.J. Transformants of Metarhizium anisopliae sf. anisopliae overexpressing chitinase from Metarhizium anisop-liae sf. acridum show early induction of native chitinase but are not altered in pathogenicity to Manduca sexta. J Invertebr Pathol 2001, 78:260-266.
17. Fan Y., Fang W., Guo S., Pei X., Zhang Y., Xiao Y., et al. Increased insect virulence in Beauveria bassiana overexpressing an engineered chitinase with the chitin binding domain from Bombyx mori. Appl Environ Microbiol 2007, 73:295-302.
18. Zhang Y., Feng M., Fan Y., Luo Z., Yang X., Wu D., et al. A cuticle-degrading protease (CDEP-1) of Beauveria bassiana enhances virulence. Biocontrol Sci Techn 2008, 18:551-563.
19. Cho E.M., Boucias D., Keyhani N.O. EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. II. Fungal cells sporulating on chitin and producing oosporein. Microbiology-Sgm 2006, 152:2855-2864.
20. Cho E.M., Liu L., Farmerie W., Keyhani N.O. EST analysis of cDNA libraries from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. I. Evidence for stage-specific gene expression in aerial conidia, in vitro blastospores and submerged conidia. Microbiology-Sgm 2006, 152:2843-2854.
21. Svitil A.L., Kirchman D.L. A chitin-binding domain in a marine bacterial chitinase and other microbial chitinases: implications for the ecology and evolution of 1,4-beta-glycanases. Microbiology 1998, 144:1299-1308.
22. Ikegami T., Okada T., Hashimoto M., Seino S., Watanabe T., Shirakawa M. Solution structure of the chitin-binding domain of Bacillus circulans WL-12 chitinase A1. J Biol Chem 2000, 275:13654-13661.
23. Arakane Y., Zhu Q., Matsumiya M., Muthukrishnan S., Kramer K.J. Properties of catalytic, linker and chitin-binding domains of insect chitinase. Insect Biochem Mol Biol 2003, 33:631-648.
24. Itoh Y., Kawase T., Nikaidou N., Fukada H., Mitsutomi M., Watanabe T., et al. Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function. Biosci Biotechnol Biochem 2002, 66:1084-1092.
25. Iseli B., Boller T., Neuhaus J.M., Iseli B. The N-terminal cysteine-rich domain of tobacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity. Plant Physiol 1993, 103:221-226.
26. Taira T., Ohnuma T., Yamagami T., Aso Y., Ishiguro M., Ishihara M. Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci Biotechnol Biochem 2002, 66:970-977.
27. Danielli A., Loukeris T.G., Lagueux M., Muller H.M., Richman A., Kafatos F.C. A modular chitin-binding protease associated with hemocytes and hemolymph in the mosquito Anopheles gambiae. Proc Natl Acad Sci USA 2000, 97:7136-7141.
28. Gorman M.J., Andreeva O.V., Paskewitz S.M. Sp22D: a multidomain serine protease with a putative role in insect immunity. Gene 2000, 251:9-17.
29. Katsushiro M., Eiji N., Hiroyuki I., Takaji S., Takeshi K., Chiaki I., et al. Molecular analysis of the gene encoding a novel chitin-binding protease from alteromonas sp. Strain O-7 and its role in the chitinolytic system. J Bacteriol 2002, 184:1865-1872.
30. Sidhu S.S., Kalmar G.B., Willis L.G., Borgford T.J. Streptomyces griseus protease C: a novel enzyme of the chymotrypsin superfamily. J Biol Chem 1994, 269:20167-20171.
31. Fang W., Zhang Y., Yang X., Zheng X., Duan H., Li Y., et al. Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker. J Invertebr Pathol 2004, 85:18-24.
32. Fan Y., Zhang Y., Yang X., Pei X., Guo S., Pei Y. Expression of a Beauveria bassiana chitinase (Bbchit1) in Escherichia coli and Pichia pastoris. Protein Expr Purif 2007, 56:93-99.
33. St.Leger R.J., Charnley A.K., Cooper R.M. Characterization of cuticle-degrading proteases produced by the entomopathogen Metarhizium anisopliae. Arch Biochem Biophys 1987, 253:221-232.
34. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
35. Mauch F., Lee A.H., Thomas B. Ethylene: symptom, not signal for the induction of chitinase and β-1,3 glucanase in pea pods by pathogens and elicitors. Plant Physiol 1984, 76:607-611.
36. Jin K., Zhang Y., Luo Z., Xiao Y., Fan Y., Wu D., et al. An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker. Biotechnol Lett 2008, 30:1379-1383.