Oligonucleotide bioanalysis: Sensitivity versus specificity

Bioanalysis

Volumn 3, Issue 12, 2011, Pages 1299-1303

  Wang, Laixin ^a  

^a LABORATORY CORPORATION OF AMERICA   (United States)

Author keywords

Bioanalysis; hybridization ELISA; LC?MS MS; oligonucleotide; siRNA; UPLC

Indexed keywords

OLIGONUCLEOTIDE; SINGLE STRANDED DNA; SINGLE STRANDED RNA; SMALL INTERFERING RNA;

ACCURACY; AMINO ACID ANALYSIS; AMINO ACID METABOLISM; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME STABILITY; HIGH THROUGHPUT SCREENING; HYBRIDIZATION; LIQUID CHROMATOGRAPHY; LIQUID LIQUID EXTRACTION; MASS SPECTROMETRY; MOLECULAR PROBE; MOLECULAR WEIGHT; QUANTITATIVE ANALYSIS; RELIABILITY; REVIEW; SENSITIVITY AND SPECIFICITY; SEPARATION TECHNIQUE; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET RADIATION;

CHROMATOGRAPHY, LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDES; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY;

EID: 79959437687     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.11.111     Document Type: Review

References (17)

1. Tremblay GA, Oldfield PR. Bioanalysis of siRNA and oligonucleotide therapeutics in biological fluids and tissues. Bioanalysis 1(3), 595-609 (2009).
2. Efler SM, Zhang L, Noll BO, Uhlmann E, Davis HL. Quantitation of oligodeoxynucleotides in human plasma with a novel hybridization assay offers greatly enhanced sensitivity over capillary electrophoresis. Oligonucleotides 15(2), 119-131 (2005). (Pubitemid 40967417)
3. Yu RZ, Baker B, Chappell A, Geary RS, Cheung E, Levin AA. Development of an ultrasensitive noncompetitive hybridization ligation enzyme-linked immunosorbent assay for the determination of phosphorothioate oligodeoxynucleotide in plasma. Anal. Biochem. 304(1), 19-25 (2002). (Pubitemid 34492838)
4. Shenk TE, Rhodes C, Rigby PW, Berg P. Biochemical method for mapping mutational alterations in DNA with S1 nuclease: the location of deletions and temperature-sensitive mutations in simian virus 40. Proc. Natl Acad. Sci. USA 72(3), 989-993 (1975).
5. Li ZJ, Li W, Dai G. Application of LC-MS for quantitative analysis and metabolite identification of therapeutic oligonucleotides. J. Pharmaceut. Biomed. Analy. 44 (2), 330-341 (2007). (Pubitemid 46887636)
6. Deverre JR, Boutet V, Boquet D, Ezan E, Grassi J, Grognet JM. A competitive enzyme hybridization assay for plasma determination of phosphodiester and phosphorothioate antisense oligonucleotides. Nucleic Acids Res. 25(18), 3584-3589 (1997). (Pubitemid 27390175)
7. Wang L. Bioanalysis of oligonucleotide therapeutics: LC-MS/MS allows for accurate and sensitive HT quantification. Genet. Eng. Biotechn. News 31(2), 26-27 (2011).
8. Deng P, Chen X, Zhang G, Zhong D. Bioanalysis of an oligonucleotide and its metabolites by liquid chromatography- tandem mass spectrometry. J. Pharm. Biomed. Anal. 52, 571-579 (2010).
9. Wang L, Yuan W, Zhao Y, et al. Quantitation of TL0901 oligonucleotide in human plasma using LC-MS/MS. Presented at: AAPS Annual Meeting and Exposition. Los Angeles, CA, USA 8-12 November (2009).
10. Zhang G, Lin J, Srinivasan K, Kavetskaia O, Duncan JN. Strategies for bioanalysis of an oligonucleotide class macromolecule from rat plasma using liquid chromatography-tandem mass spectrometry. Anal. Chem. 79(9), 3416-3424 (2007). (Pubitemid 46717172)
11. Li Z, Schariter JA, Zhang J, Davis JC, Leone AM. Application of ultra-high performance liquid chromatography for chemical characterization of liposome-based therapeutic small-interference RNA. American Pharmaceutical Review 102-110 (2010).
12. Chen C, Wang L, Zhang Y, et al. Quantitative determination of AS1411 oligonucleotide in monkey plasma using UPLC-PDA. Presented at: ISSX 15th Annual Meeting. San Diego, CA, USA 11-15 October (2009).
13. Tremblay G, Khalafaghian G, Legault J, Nielsen P, Bartlett A. Dual ligation hybridization assay for the specific determination of oligonucleotide therapeutics. Bioanalysis 3(5), 499-508 (2011).
14. Ivleva VB, Yu YQ, Gilar M. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and UPLC-MSE analysis of RNA oligonucleotides. Rapid Commun. Mass Spectrom. 24(17), 2631-2640 (2010).
15. Smith M. Characterization of a modified oligonucleotide together with its synthetic impurities using accurate mass measurements. Rapid Commun. Mass Spectrom. 25(4), 511-525 (2011).
16. Zou Y, Tiller P, Chen IW, Beverly M, Hochman J. Metabolite identification of small interfering RNA duplex by high-resolution accurate mass spectrometry. Rapid Commun. Mass Spectrom. 22(12), 1871-1881 (2008). (Pubitemid 351947753)
17. Yu Z, Baker BF, Wu H. Nuclease-based method for detecting and quantifying oligonucleotides. Isis Pharmaceuticals Inc.: WO/2002/059137 (2003).